We narrowed to 970 results for: Abo;
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TypeBlog Post... FP fragments and, preferably, some information about the structures of the tested PIPs. Many split FPs...neurodegeneration. He now spends his time learning and writing about science whenever he's not off the grid. References...
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The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data
TypeBlog Post...in his laboratory. Learn more here. Additional resources on the Addgene blog Learn more about plasmid... -
Isolating Genomic Regions of Interest with the CRISPR System
TypeBlog Post...specific genomic region In 2013, the Hodaka Fujii Laboratory first described a method to purify a specific...additional research conducted in the Hodaka Fujii Laboratory, please also see our interview with Dr. Fujii... -
Plasmids 101: Choosing an Antibiotic Resistance Gene
TypeBlog Post...therefore prevent cell death. One of the cool things about amp is that it's exclusively toxic to dividing cells...antibiotic selection. If you feel like multiple of the above antibiotics would work for you, they likely do! ... -
Better Dyeing Through Chemistry & Small Molecule Fluorophores
TypeBlog Post... the frontiers of biological imaging. *Worried about your budget? Email [email protected] Resources on the Addgene Blog Learn About Other Protein Tags Catch Up on Your Fluorescent ... -
Tips for Screening with Yeast Two Hybrid Systems
TypeBlog Post... Plasmids Read our Blog Post About Yeast Vectors Read our Blog Post About Tagging Yeast Genes ... -
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)
TypeBlog Post... activity, and much more. Read on to learn more about GFP, how scientists have evolved this versatile ...effects of alterations to this activity. Learn more about optogenetics at OpenOptogenetics and find optogeneic... -
Plasmids 101: Shuttle Vectors
TypeBlog Post...your experiment, meaning you don’t have to worry about vector replication at all. In these cases, transient...place in. In many cases, you won’t need to worry about the expression of your gene of interest in both ... -
CRISPR 101: Targeting RNA with Cas13a (C2c2)
TypeBlog Post...potential applications of Cas13a given what we know about its structure and function? For starters, Cas13a... Check Out Our CRISPR Featured Topic Page Learn about Anti-CRISPRs Multiplex Genome Editing with Cpf1 ... -
Plasmids 101: Gateway Cloning
TypeBlog Post... expression systems. Keep reading to learn more about the Gateway cloning method and its advantages. An... on the Addgene Blog Try Out FastCloning Learn About Methylation and Restriction Enzymes Use Colony PCR... -
CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?
TypeBlog Post... 101 Series Learn about the PAM Requirements of Different Cas9 variants Learn about CRISPR Pooled Library... -
Four Base Editing Reporters to Monitor and Enrich Editing in Real-time
TypeBlog Post...for base-editing activity The reporters mentioned above all rely on GFP fluorescence and cell sorting using... Additional resources on the Addgene blog Read about other CRISPR reporters Browse our CRISPR blog posts... -
Deciphering the Mysteries of Behavior with Viral Vectors
TypeBlog Post...distant regions. They also don’t give any clues about the types of neurons involved or whether their involvement... Viral Vectors Resources on Addgene.org Learn about Addgene's viral service Find viral protocols and... -
How to Be an Excellent Trainee
TypeBlog Post...individuals who are polite, appreciative, and clear about their needs. Remember, your bench mentor’s training...prepared and take training seriously, as reviewed above. Offer to help clean up and/or make necessary... -
Tips for CRISPR Gene Editing in Mice
TypeBlog Post...the pX330 plasmids used in the validation assay (above) and it is just as effective (Mashiko et al., 2014...Engineering with CRISPR Validate Your Genome Edit Learn about Non Homologous End Joining and Homology Directed... -
Getting the Most from Your Lentiviral Transduction
TypeBlog Post...Lentiviruses are a powerful laboratory tool often employed to establish cell lines that stably express...Get Tips for Titering Your Lentivirus Learn All About Viral Vector Elements Learn How to Do Genome-Wide... -
Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs
TypeBlog Post...moxFPs over standard FPs for the reasons listed above. In addition, because the moxFPs are highly monomeric...Medicine. Her doctoral research was conducted in the laboratory of Erik Lee Snapp and focused on optimizing fluorescent... -
Antibodies 101: Beyond Surface Labeling
TypeBlog Post..., you may want to look at other methods. In the above cases, a good alternative is using a two-protein... region of the primary antibody (Figure 1b). As above, the advantage of this system is increased flexibility... -
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus
TypeBlog Post...first time AAV user Learn about how AAV is used in retinal gene therapy Learn about the parts of an AAV transfer... -
New Optogenetic Tools for Cytoskeleton and Membrane Control
TypeBlog Post... Check out our Optogenetics Guide to learn more about how these modules work, and read on to learn how...to the cell membrane isn’t powerful enough, what about altering the chemical composition of the membrane...