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...or the PNS, respectively.  Read on to learn more about these two new AAV capsids and how they can be useful...Important considerations when using AAVs Learn more about using AAV as a tool for gene expression in mammals...

... the frontiers of biological imaging.  *Worried about your budget? Email [email protected] Resources on the Addgene Blog Learn About Other Protein Tags Catch Up on Your Fluorescent ...

... Plasmids Read our Blog Post About Yeast Vectors Read our Blog Post About Tagging Yeast Genes ...

... activity, and much more. Read on to learn more about GFP, how scientists have evolved this versatile ...effects of alterations to this activity. Learn more about optogenetics at OpenOptogenetics and find optogeneic...

...for base-editing activity The reporters mentioned above all rely on GFP fluorescence and cell sorting using... Additional resources on the Addgene blog Read about other CRISPR reporters Browse our CRISPR blog posts...

... 101 Series Learn about the PAM Requirements of Different Cas9 variants Learn about CRISPR Pooled Library...

... expression systems. Keep reading to learn more about the Gateway cloning method and its advantages. An... on the Addgene Blog Try Out FastCloning Learn About Methylation and Restriction Enzymes Use Colony PCR...

...individuals who are polite, appreciative, and clear about their needs. Remember, your bench mentor’s training...prepared and take training seriously, as reviewed above. Offer to help clean up and/or make necessary...

...your experiment, meaning you don’t have to worry about vector replication at all. In these cases, transient...place in. In many cases, you won’t need to worry about the expression of your gene of interest in both ...

...distant regions. They also don’t give any clues about the types of neurons involved or whether their involvement... Viral Vectors Resources on Addgene.org Learn about Addgene's viral service Find viral protocols and...

...potential applications of Cas13a given what we know about its structure and function? For starters, Cas13a... Check Out Our CRISPR Featured Topic Page Learn about Anti-CRISPRs Multiplex Genome Editing with Cpf1 ...

...the pX330 plasmids used in the validation assay (above) and it is just as effective (Mashiko et al., 2014...Engineering with CRISPR Validate Your Genome Edit Learn about Non Homologous End Joining and Homology Directed...

...Lentiviruses are a powerful laboratory tool often employed to establish cell lines that stably express...Get Tips for Titering Your Lentivirus Learn All About Viral Vector Elements Learn How to Do Genome-Wide...

...moxFPs over standard FPs for the reasons listed above. In addition, because the moxFPs are highly monomeric...Medicine. Her doctoral research was conducted in the laboratory of Erik Lee Snapp and focused on optimizing fluorescent...

...first time AAV user Learn about how AAV is used in retinal gene therapy Learn about the parts of an AAV transfer...

... Check out our Optogenetics Guide to learn more about how these modules work, and read on to learn how...to the cell membrane isn’t powerful enough, what about altering the chemical composition of the membrane...

..., you may want to look at other methods. In the above cases, a good alternative is using a two-protein... region of the primary antibody (Figure 1b). As above, the advantage of this system is increased flexibility...

...bind the RNA motif will be biotinylated. Read more about RapID in this blog! Find plasmids for RapID EXCELL..., S. C., Noble, K. A., KC, B., Roux, K. H., Motamedchaboki, K., & Roux, K. J. (2016). An improved smaller...

...Featured Topic Page Learn about Non-Homologous End Joining Learn about Base Editing Read 3 Tips ...

...PUF Assembly Kit Below you’ll find information about what these kits contain, how they can be used, and...encoded sensors enable real-time observation of metabolite production." Proceedings of the National Academy...