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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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In Category

We narrowed to 960 results for: App

Showing: 761 - 780 of 960 results

  1. Ligation Independent Cloning

    Type
    Protocol
    ...traditional cloning, base-pairing in the short overlapping regions (usually 4 bp) does not provide enough...with the start codon or tag sequences (where appropriate). The primer length is dependent on the T4 Pol...

  2. Gibson Assembly Protocol

    Type
    Protocol
    ...fragment length or end compatibility, multiple overlapping DNA fragments can be joined in a single isothermal...part to be inserted is too long to include on overlapping PCR primers (>60 bp) but too short to make its...

  3. Lentivirus Production

    Type
    Protocol
    ...lentivirus can be used for a variety of downstream applications such as stable-cell line generation. Workflow...mL of DMEM Complete to a 15 mL conical and an appropriate amount of 25 mM chloroquine to the conical so...

  4. Colony Formation Titering Assay

    Type
    Protocol
    .... Add 1.5 mL of DMEM complete containing the appropriate antibiotic. Incubate the cells for ~2 weeks. ...replace it with fresh DMEM complete containing the appropriate antibiotic. When the untransduced (negative control...

  5. Pipetting Protocol

    Type
    Protocol
    ... P1000. The table shows the pipette with the appropriate dispense volume: This image shows the P2, P10...,000 µL Each pipette should be used with the appropriate tips, which are made in a number of sizes and...

  7. Western Blot

    Type
    Protocol
    ... can be adapted for wet and semi-dry transfer approaches. Last Update: January 24, 2022 Workflow Timeline...remove supernatant. Resuspend cell pellet in appropriate volume of cold lysis buffer. Pro-Tips The volume...

  8. AAV ddPCR Titration

    Type
    Protocol
    ...Water, Hyclone, SH30538.02 GeneAmp 10X PCR Buffer, Applied Biosystems, N8080129 ddPCR Supermix for Probes ... PCR tubes. Add 5 µL of dilutions 6–8 to the appropriate PCR tubes. Pipette back and forth 5 times. Lightly...

  9. Kit Free RNA Extraction

    Type
    Protocol
    ... not affect the quality of RNA or downstream applications. To improve yield of RNA, instead of incubating...

  12. CRISPR Guide Update

    Type
    Blog Post
    ...introduction to what CRISPR technologies have to offer. Happy CRISPR-ing! More resources on the Addgene blog ...

  15. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    ...variants. Next, we’ll look at various CRISPR applications (such as visualize, screen, activate, and repress...

  16. Addgene's 20th Anniversary Party

    Type
    Blog Post
    ...questionable collection of memorabilia, even made an appearance during co-founder and CSO Melina Fan's words ...

  17. We’re Going Viral This Week!

    Type
    Blog Post
    ... information on the basics of viral vectors, applications, and some new resources available from Addgene...
Showing: 761 - 780 of 960 results