We narrowed to 821 results for: EED

... to help researchers choose the probe for their needs. You’ll find basic information on NIR FPs, their...

...single-chain Fv analogue produced in Escherichia coli. Proceedings of the National Academy of Sciences 85:5879–5883...

...tension, however, the researchers realized they needed specific tools to interpret the roles of individual...

...molecule containing your desired edit are all you need to generate a knock-in.   General considerations...

...select for desirable traits using traditional plant breeding approaches, these techniques are cumbersome, often...

...the ORF to be expressed Finally, the researcher needs a plasmid encoding their open reading frame of interest...

...plasmid. A low copy-number plasmid, encodes proteins needed for bacterial conjugation. Genes listed on F´ are...

... limitations create challenges for measurements needed to move discoveries from the lab bench into drug...

...-based state machines for your own experimental needs. For more context, exposition, and detail, please...

...and other organisms lacking this innate immunity need not worry and have benefited from the ease of use...

...closest friends and have often provided me with much-needed emotional support during my journey. When you are...

.... demonstrated that the Cas9 K866PCK mutant was indeed inactive prior to irradiation with UV light (365nm...

...transport relies on kinesin. Different rates of speed for various forms of retrograde and anterograde ...

... easier for you to find and use the plasmids you need. You can find all the hot plasmids from 2017 below...

...2022 Workflow Timeline Day 1: Seed cells Day 2: Transfect cells Day 3-6: Feed cells Day 7: Harvest antibody...fresh solutions after one month. BCD Feed 500 mL BalanCD HEK293 Feed 20 mL 200 mM Glutagro Store at 4 °C... °C. Procedure Section 1: Seeding cells The day prior to transfecting, seed a 108 mL culture of HEK293...post-transfection, supplement the flask with 4% BCD Feed. Pro-Tip The feed can be repeated up to 4 times for a total...Example feeding schedule: Thursday: Transfect cells. Friday (24 h post-transfection): Add 4% BCD Feed. Monday...to 3.75 mM, add 4% BCD Feed. Tuesday (120 h post-transfection): Add 4% BCD Feed. Wednesday (144 h post-transfection...purified for use in a variety of applications. Sharing speeds science. We believe that sharing the full details...

...control panel where you can set the time and speed needed for your experiment. Procedure An example of...centrifuge needed. This decision should be based on the size of tubes you are using and the speed at which...according to speed and time (and temperature, if applicable) listed in your protocol. Pro-Tip Spin speed is often...which is generated by spinning the sample at a high speed. Being able to separate solids from a liquid or ...accommodate different sized containers, spin at different speeds, or keep samples at specific temperatures. This...conical tubes. Depending on the centrifuge their speed ranges can vary, but are typically lower than some... about bench height. They often spin at similar speeds to their smaller cousins, but can hold much larger...

...QuickSeal tube spacers 16 ga needle 18 ga needle 10 mL syringe 18 ga blunt edge needles, Hamilton 1X PBS pH 7.4...Figure 2) with an 18 ga needle. Place the first microcentrifuge tube under the needle’s opening to collect ...interface with an 18 ga needle attached to a 10 mL syringe. The bevel of the needle should be up, facing ...a 10 mL syringe and a blunt edge 18 ga Hamilton needle, taking care to avoid bubbles (Figure 1). 8 mL ... 90 min in a T70i rotor at 10 °C. Pro-Tip If you need more time, you can alternatively centrifuge for ...Puncture the top of the QuickSeal tube with a 16 ga needle and start collecting 0.5 mL to 1 mL fractions per...AAV-containing Iodixanol solution will flow from the needle set at the 40–60% interface. Make sure that the...

...175 flasks. Workflow Timeline Day 0: Seed cells in CS2 Day 2: Seed cells in CS5 Day 3 (am): Transfect cells... for 24 h–36 h. Proceed with transfection: Calculate the amount of each plasmid needed to have a 1:1:1...plasmid we need: Sample Calculations RepCap: 0.08 μg/bp × 7,265 bp = 582.1 μg Volume Needed: 582.1 μg ...Precipitation of the viruses can proceed overnight at 4 °C if needed. Transfer the entire sample to 3 ...volume to 300 mL with DMEM complete media and mix. Seed all cells in 1 CS2. Return to incubator for 48 h...Count cells using a hemocytometer or cell counter. Seed 350 million cells from the CS2 into one CS5 with...three plasmids, we can determine the total μg/bp we need to achieve a 1:1:1 molar ratio of each plasmid: ...

...optional) Seed cells for the generation of conditioned medium (see below for more details) Day 1: Seed individual...mL To seed one 96-well plate, make 10 mL of a 5 cell/mL solution. Calculate the total cells needed: Total...doing this, you are seeding the plate at an average density of 0.5 cells/well. Seeding an average of 0.5...Procedure Generating Conditioned Medium (optional) Seed stable cells such that the next day they will be...cells in a 10 cm dish. Each 10 cm dish should be seeded in 10 mL DMEM complete, which will generate enough...cell solution for each 96-well plate you plan to seed. If you choose not to use conditioned medium, prepare...medium. This 5 cells/mL solution will be used to seed the 96-well plate. Sample Calculation First, use...

... chemical reactions such as restriction digests needed in molecular biology, and thaw cell lines. There...Thermometer Water bath weights and floats Reagents None needed Procedure Ensure that the water bath and water ...clean it regularly. Determine the amount of water needed to fill the water bath, and be sure to know what...will reach the desired temperature by the time you need it for your experiment. Water baths tend to heat...up the water bath 30 minutes to 1 hour before you need to use it to allow the temperature to stabilize....containers that will go into the water bath. If you’ll need to sterilize your items after removing them from...tube. This way, as items are floating, you do not need to necessarily maneuver the bottles or tubes to ...