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We narrowed to 1,083 results for: IND

Showing: 761 - 780 of 1083 results

  1. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    ...located in. Otherwise it could take us hours just to find one sample! Luckily, if a rack of tubes is dropped... barcode also identifies the contents of each individual tube.  If a plate of samples is ever dropped,...know what is in each one. Even if we lose a tube behind a freezer or under a lab bench, we will know that...container a specific location to know exactly where to find your samples. Additionally, giving each sample a...

  2. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...37C Y66W Causes the chromophore to form with an indole rather than phenol component (cyan derivatives)...one fused to each protein or domain being tested. Find FRET plasmids here. Split EGFP: An alternative to... more about optogenetics at OpenOptogenetics and find optogeneic actuators and sensors at Addgene. Cell... Gal will share his list of 10 things to keep in mind when choosing the best fluorescent protein to use...

  3. Bioinformatics at Addgene

    Type
    Blog Post
    ...the years we have accumulated more than 300,000 individual nucleotide sequences. More than half of these...Quick-stepping to a FASTA file   With our outline of individual steps to automate plasmid sequence assembly, ... of one-off scripts or programs, our goal was to find a suitable software tool that could orchestrate ... More resources on the Addgene blog:  The Power Behind NGS Plasmid Validation: SeqwellHow to Keep a Lab...

  4. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ... also depends on the individual library. So, on each library’s page, you can find protocols for sequencing...amplification. And as anyone who has worked with individual plasmids knows, some plasmids amplify well and...troubleshooting blog should you run into any issues.  Keep in mind that a low MOI will increase the chances of a cell...

  5. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...immunology or subpopulations of cells, you’ll soon find yourself encountering flow data in the literature... will have numbers by gated populations; this indicates the percentage of total events contained in the... gated population. Heat maps are often used to indicate the relative density of cells. You can see both...presented. A horizontal line across the peak will indicate the cut-off value for a positive signal (Figure...

  6. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...various research applications, they have become indispensable tools for manipulating cells and organisms. ... to cells for genome editing, and introducing inducible systems into cells, among many other uses. Lentiviral...lentiviral production protocol.  One thing to keep in mind is that titering these IDLVs may require a slightly... (2013). High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells. Nature ...

  7. March for Science

    Type
    Blog Post
    ...talk with them about science: Representatives: To find contact information for your representative in the...you need to know is your area code. Senators: To find contact information for your senators all you need... do is select your state. State Legislatures: To find the website to contact representatives at the state...objectivity of science.  Scientific American: Scientists Find a Voice at Massive Rally for Immigrants National...

  8. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    ...that can be used with mutational scanning: ligand binding assays, phage display assays, ubiquitination activity...activity assays, toxin activity, small molecule binding, growth rate, and many more. See Fowler et al for...a single mutagenic primer pair to generate an individual mutant, this technique uses mutagenic primers... Mutate Your Plasmids Resources on Addgene.org Find Plasmids from the Whitehead Lab Use CRISPR Pooled...

  9. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ...presenters! Labs and benches! Everything was out the window. So, much like SynBio, it was time to start our... was going to be time consuming and difficult. Finding volunteers who had space to shoot was another hurdle...people enough.  Build: If you’ve ever watched any behind the scenes footage in the last several years, you...distance. Since everyone in these shots (and me behind the camera) would remain masked, it freed us up...

  10. Five Popular Model Organisms

    Type
    Blog Post
    ...cancer cells more resistant to chemotherapy. You can find these plasmids at Addgene. Another advantage of ...model organism in scientific research. Yes, the same kind we use in breads and other baked goods! Yeast is...plasmids. Then using HOTcre, a method that uses heat induction to control the temporal expression of different...collection pages Read our molecular biology reference Find plasmids for your research ...

  11. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...Resources on Addgene.org Find CRISPR Plasmids for Plants Read Our CRISPR Guide Pages Find Validated gRNAs for... for targeting your favorite plant gene; you can find many plasmids for a wide variety of CRISPR applications...Agrobacterium-Mediated Plant Transformation: the Biology behind the “Gene-Jockeying” Tool. MMBR 67:16–37 . https...

  12. Evolution of Lab Techniques

    Type
    Blog Post
    ...researcher is searching for), then the probe will bind to the sample thereby labeling it. The label can...southern blotting are conducted on a sample from an individual without sickle cell anemia, two DNA fragments...sequencing could move from sequencing cores to individual labs and even out into the field. Current versions...Resources on Addgene.org Check out Our Protocol Pages Find Molecular Biology Tools Analyze Your Plasmids by...

  14. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    ...2015) made this step as simple as heat-shocking to induce the expression of a Cre recombinase contained within...editing efficiencies, obtaining as many as 100 independent GFP knock-ins from a single injected animal.  ...elegans has an AT-rich genome, it can be difficult to find an appropriate NGG PAM site to target. The (T)TTN...visualize specific genomic loci (CRISPR-imaging), or to find the proteins associated with a given genomic locus...

  15. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...examples of survival of the fittest. Cancer cells find a way to grow haywire, access and create more vasculature...inflammatory responses can augment tumor growth. Indeed, lasting responses to viral infections have resulted...lack of oxygen in the tumor, activates hypoxia inducing factor (HIF-1), which promotes the production ...response against tumors. Some chemotherapeutic agents induce immunogenic tumor cell deaths that help activate...

  16. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    ...Fluorescent Protein Resources Find Empty Fluorescent Protein Backbones Find Plasmids for Optogenetics ...system relies primarily on contrast to identify individual objects and perceive the world around us. Without...reflected. This reflection occurs due to the refractive index mismatch between the glass and the water-like buffer...

  17. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    ...consistent annoyance of walking into a fly lab and finding ourselves accosted by dozens of tiny black dots...This innovation system can help fly labs everywhere find and re-contain any specimens that escape during ...   If you're working in fly research, I hope you find this new technology as helpful as we have!  References...

  18. We’re Going Viral This Week!

    Type
    Blog Post
    ...graphic below. Follow the hashtag #AddgeneGoesViral to find information on the basics of viral vectors, applications...Hub, an open platform for scientists using AAV to find and contribute experimental information, such as...Addgene blog Download our Viral Vectors 101 eBook Find all of our viral vectors blog posts Learn more about...

  19. Enabling high school research at the Journal of Emerging Investigators

    Type
    Blog Post
    ...scientific research. You’ll meet some of the folks behind the journal and hear from a fantastic team of high...      Additional Resources from the Addgene Blog Find education resources Use our Plasmids 101 eBook to...enegineering technology Resources at Addgene.org Find basic molecular biology protocols Check out our ...
Showing: 761 - 780 of 1083 results