We narrowed to 971 results for: LARS
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TypeBlog Post...Monoclonal antibody drugs are popular therapeutics for a plethora of disease conditions, from cancer ...Immunotoxicology Testing of Monoclonal Antibodies in Macaca fascicularis: From Manufacturing to Preclinical Studies. ...
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K. phaffii: Rising to the Occasion
TypeBlog Post...evolutionarily it evolved much slower and bears more similarity to the ancient yeast strains and metazoan cells...Additional Resources on the Addgene Blog Five Popular Model Organisms Plasmids 101: Yeast Vectors ... -
Networking Follow-up: I Met a Person. Now What?
TypeBlog Post...conferences/meetings over and over to get to know the regulars (or better yet, become a volunteer). Naturally...stay in touch with all of their connections on a regular basis. It is also not at all necessary. Once you... -
Learning Technical Skills at a Lab Central Workshop
TypeBlog Post...central We’re working on a project where we aim to do large scale DNA preps with multiple samples without cross...time and space in your own lab, you should give similar workshops a try if you’re ever looking to try out... -
Virtual Science Conference Coming Up? Three Addgenies Share Their Experience
TypeBlog Post...sessions, not your walks between sessions Like many large conferences with multiple sessions happening at ...walking up to the mic and asking your question in a large conference room puts butterflies in your stomach... -
Scientists Map the SARS-CoV-2-Human Interaction Network
TypeBlog Post...would take years and millions, if not billions, of dollars, but by approaching it from a new direction, we...Science Communications Team at QBI UCSF. They are particularly interested in facilitating collaborative science... -
Summer SciComm Series: A PhD in Science Communication
TypeBlog Post...greater science literacy and education have more polarized beliefs on controversial science topics. Proceedings..., L. L., Braman, D., & Mandel, G. (2012). The polarizing impact of science literacy and numeracy on perceived... -
DAP arrays for multiplex gene editing
TypeBlog Post...prime editing, achieving up to 3-loci MPE with similar high efficiencies, which is scalable for a higher...genes against multiple genetic diseases, such as muscular dystrophies, heart disease, and type 2 diabetes... -
Using CRISPR/Cas9 to Edit Disease Out of the Genome
TypeBlog Post...are being given in vivo, it is difficult to use larger enzymes. To reduce the impact of off-target mutations...B. 2021. “CRISPR/Cas gene therapy.” Journal of cellular physiology. https://doi.org/10.1002/jcp.30064... -
New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor
TypeBlog Post...sensor to the membrane and displays it on the extracellular side. In addition to the original PDGFR TMD,...promoter, FLP-dependent expression GFAP: Glial fibrillary acidic protein (promoter). Good for Glial expression... -
Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS
TypeBlog Post... tissue-specific promoter sounds simpler, this modular system saves you from having to create a new fly...and overexpress viral genes. Additionally, other modular expression systems exist (eg. LexA/LexAop and Cre... -
5 Great Apps for Lab Life
TypeBlog Post... names have a B, J, X, or Z. Some alternative, similar apps: Findings Lab Notebook Cell Culture Split...Protoctols for Your Research Catch Up On Your Molecular Biology Basics ... -
How to Lead a Great Meeting
TypeBlog Post... dialogue and community among participants, clarification - new and useful information, and invigoration...meetings. One-on-Ones: Managers should set up regular check-ins with their employees. 15-30 minutes weekly... -
Fluorescent Proteins 101: Luciferase
TypeBlog Post.../Phototoxicity Not susceptible Susceptible Subcellular Imaging Improving Well-established High-throughput...effectiveness. Analyze Cell Signaling Pathways Known cellular signaling pathways can be investigated in a high-throughput... -
Kit Free RNA Extraction
TypeProtocol...Kit-Free DNA Purification Agarose Gel Purification Molecular Biology Reference Introduction This protocol describes... (Sarkosyl) 0.1 M 2-mercaptoethanol TRIzol® or similar product such as TRI Reagent®, RNAzol®, QIAzol® ...Homogenize or lyse tissues or cells in TRIzol® or a similar product. For tissues: use 1 mL of TRIzol® per 100... -
Flow Cytometry Readouts: Yes, No, and Everything in Between
TypeBlog Post...can adjust this by changing the voltage for the particular fluorophore prior to recording data. Increasing...you are looking at proteins that are expressed regularly in cells and might be upregulated or downregulated... -
Sweating the Small Stuff: Details in the Lab
TypeBlog Post... Prevent Interruptions If you need to focus, a large set of headphones can act as a Do Not Disturb sign...would build a wall of pipette tip boxes when she particularly needed to focus, which was extremely effective... -
Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification
TypeBlog Post...light-based control of signaling pathways. This is particularly exciting because using light to control signaling...With darkness, SOS binds Ras and initiates the extracellular signaling-regulated kinase (ERK) signaling cascade... -
Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors
TypeBlog Post... through my work on the audit committee. I’m particularly curious to see how that progresses in 2019 and...Before you can go off and do great things in a particular area or profession, you need to make sure you... -
Early Career Researcher Toolbox: Social Media for Scientists
TypeBlog Post...Guide" from Nature Jobs Blog While Twitter is a popular platform for scientists, it’s not the only tool...statistics on what percent of scientists and engineers regularly use the platform, why scientists are using the...