We narrowed to 941 results for: OMP;
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TypeBlog Post...practice while seated or with your poster on your computer screen than it is to pretend you’re standing at...guilty of this, and have many times answered a completely different question than the intended one. Take...
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Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More
TypeBlog Post...few new ones that are not used so much but have comparable expression levels to things people do use a lot...example, knock out a gene with simultaneous complementation, with a knockout and expression construct on... -
Great Results Start with Great Standard Curves
TypeBlog Post... or very similar buffers if not, since buffer components can affect the final readout. When preparing ...using advanced graphing software designed to plot complex trendlines. If you don’t have access to this kind... -
CRISPR 101: Making a Knock-In Cell Line
TypeBlog Post...end joining (alt-EJ). Inhibitors to essential components of both pathways have been shown to be efficacious... al., Nat Com). Fig. 3: HDR events compete with NHEJ-mediated edits of small insertions and... -
A Guide to Starting Your Own Journal Club
TypeBlog Post...literature and emerging technologies to remain competitive and relevant. Enter: the journal club. A journal...gauged interest from relevant parties, and the company at large, by polling and informal communications... -
AAVs in Retinal Gene Therapy
TypeBlog Post...correct genetic conditions over 25 years ago when completing her PhD and medical training in Ophthalmology...time. Importantly, it is an immune privileged compartment, meaning that it can tolerate the presence of... -
Uncovering Ancestral Bias in CRISPR Research
TypeBlog Post..., but it still points to a far larger and more complex problem endemic to biomedical research: pervasive...same reference genomes and libraries. Further complicating the issue is the long and ugly history of reporting... -
CRISPR Antimicrobials
TypeBlog Post...replication that can be packaged into replication-incompetent phage particles using a helper system. These ...antimicrobials combines them with both replication-competent phages and antibiotics. Phage therapy fell out... -
Tips for Screening with Yeast Two Hybrid Systems
TypeBlog Post...transcription. The HIS3 reporter system (e.g. the Gateway-compatible bait Y2H plasmid pEZY202 from Yu-Zhu Zhang’s...particular cell type, or a subset based on a cellular compartment or growth stage). A prey library can either be... -
CRISPR 101: Any Base Transversion Editors
TypeBlog Post... Note: hypoxanthine (shown) is the nucleobase component of the nucleoside inosine. Created with BioRender.com...relied on deaminase enzymes, either to directly accomplish the base edit or to create the substrate for ... -
Antibodies 101: Reading a Flow Plot
TypeBlog Post...roughly measures granularity, a proxy for nucleic complexity of the cell. The sample will have been labeled...labeled using a panel of markers, which is comprised of antibodies conjugated to fluorescent markers and/... -
Viral Production at Addgene
TypeBlog Post...Purity Purity of AAV preparations is assayed by comparing the relative stoichiometric ratios of the viral...Absolute determination of single-stranded and self-complementary adeno-associated viral vector genome titers ... -
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone
TypeBlog Post...these people at the time, but I met some very accomplished scientists and some rising stars in the world...timetable for the Singletons in their bins. The next component of Binning Singletons is when we bring all the... -
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs
TypeBlog Post...persistence of residual viral material [6]. The complete removal of the residual viral particles and establishment... in the presence of these two factors are not completely understood, the addition of an shRNA for P53 ... -
How to Write a Scientific Review Article
TypeBlog Post...Good luck! References 1. Haery, Leila, Ryan C. Thompson, and Thomas D. Gilmore. "Histone acetyltransferases...Addgene.org Check out Our Hot Plasmids Articles to See Compiled Info on New Plasmids Review the CRISPR Field with... -
Mesothelioma - Causes, Symptoms, and Treatment
TypeBlog Post...is a terrible disease that is, unfortunately, completely preventable. Mainly caused by exposure to asbestos...non-resectable mesothelioma by the National Comprehensive Cancer Network, a coalition of the top 27 cancer... -
Plasmids 101: Codon usage bias
TypeBlog Post...varied codon preferences among organisms aren’t completely understood, but some possible reasons include...codon optimization algorithms and DNA synthesis companies such as IDT and GenScript host online codon optimization... -
Rett Syndrome: A History of Research and Therapeutic Outlooks
TypeBlog Post...in mice is possible (Guy et al., 2007). They accomplished this using Cre-Lox mouse models in which the...Cre not only halted the neurological damage but completely reversed it, regardless of age or symptom severity... -
Changing Labor Laws Bring Increased Postdoc Wages
TypeBlog Post...institutions doing? Institutions are now preparing to comply with the changes to the law on Dec 1st, according...centrally administered, which leads to many complications, including the inability of some institutions... -
Getting the Most from Your Lentiviral Transduction
TypeBlog Post...ml before the media becomes cloudy. Mycoplasma compete with host cells for nutrients and can alter expression...al. outline a do it yourself PCR-based approach complete with freely available positive controls. Routinely...