Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 1,010 results for: PLE

Showing: 761 - 780 of 1010 results

  1. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    .... For more mechanistic details on HDR and NHEJ, please see the linked blog posts. Compared to its counterparts...strategy looks very similar to HDR, but it is completed using much shorter sequence homologies. Abbreviated...Genome-Editing Based Approach for Mapping Multiprotein Complexes in Human Cells.” Cell Rep. 13: 621-633. PMID: ...

  2. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...ribonucleoprotein (RNP), consisting of the Cas9 protein in complex with a targeting gRNA, to your cells of interest...CRISPR experiment is the generation of the RNP complex.  While one option is to purchase the Cas9 protein...bread wheat using CRISPR/Cas9 ribonucleoprotein complexes." Nature communications 8 (2017): 14261. PubMed...

  4. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    .... Now GFP and its fluorescent derivatives are a staple in the lab. GFP is used in research across a vast...importance of the entire GFP protein, which is almost completely required to maintain fluorescent activity; very...exist with only slight variations separating them.  Please note that many fluorescent proteins found on the...

  6. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...polycistronic tRNA-gRNA (PTG) gene system to clone multiple protospacers which enables us to target more than...Minkenberg, and Yinong Yang. "Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing...PMC5972399. 12. Molla, K., Shih, J. & Yang, Y. Multiplex CRISPR-mediated base editing in rice. In Genome...

  7. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...granularity, a proxy for nucleic complexity of the cell.  The sample will have been labeled using a panel...parallel.  Let’s imagine we’re looking for cells in a sample that are negative for marker A and positive for...

  8. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ...light inducible ion channels (plasmid # 20298 for example) have revolutionized Neuroscience. They are the...researcher exquisite control over expression of multiple genes in specific cells at specific times. Many.... “A FLEX Switch Targets Channelrhodopsin-2 to Multiple Cell Types for Imaging and Long-Range Circuit ...

  9. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...system frequently over the past fifteen years. Some examples from their lab include using AAV to introduce ... alleles of a gene, or which require modifying multiple genes simultaneously in the same cell, are more...by using a targeted nuclease. Asuri et al, for example, used zinc fingers to increase the efficiency of...

  10. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    ...pluripotent stem cells (iPSCs). The CRISPR/Cas9 com­plex is very efficient at introducing double stranded...the PAM as possible (see below to find out why). Please check our recent studies for details (Paquet, Kwart... systems.     References 1. Cong, Le, et al. "Multiplex genome engineering using CRISPR/Cas systems." ...

  11. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...GCN4 binding scFv that’s fused to GFP. When the multiple copies of the scFv-GFP fusion bind the SunTag ..., Alitheen NBM, Hamid M (2012) scFv Antibody: Principles and Clinical Application. Clinical and Developmental....0000000000000145  Gąciarz A, Ruddock LW (2017) Complementarity determining regions and frameworks contribute...

  12. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ... any gene of interest, one should require that multiple gRNAs of different sequences give rise to the ...but can often require difficult decisions. For example, would it be better to use a less-active gRNA that... in the interpretation of results by requiring multiple sequences targeting a gene to score in order to...

  13. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...allows for targeted delivery of Tet1 alone, or coupled with DNA oxidation and repair factors that allow...more frequently inherited by daughter cells. For example, KRAB induced silencing was transient and quickly...and context-dependent, it may make sense to try multiple CRISPR tools when setting up your experimental...

  14. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...primary cellular receptor and sometimes one or multiple coreceptors, which may be present on different...and modifying capsid and genomic structures. For example, rAAV pseudotypes mix the genome of one AAV serotype...  AAV8 AAV8 has high transduction efficiency in multiple tissues, rapid transgene expression, and can cross...

  15. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...are working with proto-oncogenes or with human samples that have not been tested for HIV.  If not using...must be chosen carefully. Often researchers want a simple set-up where the gene of interest is expressed ...experience in transfection, CaCl2 transfection is a simple and cheap technique that should work well. Quickly...

  16. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...extracting the DNA from fossilized amber and supplementing it with the DNA of present-day frogs via genetic...template.  Each zinc finger recognizes a nucleotide triplet and when several ZFNs are strung together, it can...the ZFNs, but instead of recognizing nucleotide triplets, the components recognize individual nucleotides...

  17. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    ... viral DNA sequences and expression profile at multiple steps from plasmid amplification in bacteria to... set of injections into your animal model. For example, in a recent study by the Wickersham lab (Lavin...essential that every new vector, whether it be a completely new transgene or merely a new lot, be thoroughly...

  18. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    ...cassette (Dickinson et al., 2015) made this step as simple as heat-shocking to induce the expression of a ...direct injection of a CRISPR/Cas9 ribonucleoprotein complex with linear DNA as repair templates which reduces...worms are perfectly viable at 15 ºC, yet exhibit complete embryonic lethality at 25 ºC. This method resulted...

  19. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...CRISPR-based gene editing (see table below). For example, the far-red light-activated split-Cas9 (FAST) ...translocation of the protein BldD to the nucleus. To couple this light-dependent activity to transcription,...proteins by fusing Cas9 to photoreceptors. One prime example was from the lab of Michael Lin, who developed ...

  20. CRISPR 101 eBook...en español

    Type
    Blog Post
    ... Denise Roche, to edit the translation. We are pleased to announce that CRISPR 101: Una herramienta de...repository. This incredible gift will allow more people to access helpful resources for their CRISPR experiments...
Showing: 761 - 780 of 1010 results