We narrowed to 1,006 results for: RING

1. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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   ...similar resource for those involved in monocot engineering projects. The MoClo tools will work in any dicot...think plant biology is massively underfunded considering how important it is. I would really like to reach...

2. Great Results Start with Great Standard Curves

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   ...step of the series is consistent at 1:2. When preparing a dilution series, use a new pipette tip for each...components can affect the final readout. When preparing a standard curve, change pipette tips and mix ...

3. Simplify Cloning with in vivo Assembly

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   ...to get into the same cell. For simple cloning requiring just one or two recombination events, homemade...DNA polymerase This will minimize random errors during PCR: we recommend Phusion or Q5. Speed up your ...

4. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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   ...community is a kind of duty for researchers when considering the peer-review system of journals and grant ...technologies developed abroad, so it's a little boring. I think that such tendency is one of the reasons...

5. Negotiating Work and Life: How to Find the Joy

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   ...Neighborhood.   Work/life balance. Is it truly possible to bring all aspects of our lives under control? Balance ... friends. If there is any guilt, you should be sharing it absolutely equally, which means you only have...

6. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

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   ...full-length (19-20 bp) or short (17-18 bp) guides. When comparing the toxicity of guides that have 1-bp mismatch...Central PMCID: PMC3988262. 4. Hart, Traver, et al. "Measuring error rates in genomic perturbation screens: gold...

7. How to Write a Scientific Review Article

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   ...about. Instead of reading a paper and getting my bearings, I would read a paper, panic, and then download...think the field is going or what areas are worth exploring.  Edit and rewrite, then repeat If you’re like...

8. Mesothelioma - Causes, Symptoms, and Treatment

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   ...mesothelioma. Asbestos, and certain other naturally occurring asbestiform minerals such as erionite, are the...an immediate stop to the use of asbestos, thus ensuring that future generations will be protected from...

9. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

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   ...pinhead—contribute to the “sculpting” of tissues and organs during embryo development. Single cell sequencing-based...Single-cell reconstruction of developmental trajectories during zebrafish embryogenesis." Science 360.6392 (2018...

10. Hot Plasmids - August 2020

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    ...repressing and activating expression of TCF4 while monitoring transcript and protein levels. Plasmids for multiplexed...donor infection (CRISPR-READI) enables genome engineering using HDR donors lengths up to 4.9 kb. Find ...

11. Plasmids 101: Shuttle Vectors

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    ...biology because of an image that was honestly quite boring. My Bio 101 professor displayed a codon table —...mammalian cells like neurons, may not replicate during the course of your experiment, meaning you don’...

12. CRISPR 101: Any Base Transversion Editors

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    ... target guanosine instead, perhaps analogous engineering of UNG, which excises uracil, could allow it ...considerably. And there’s so much more exciting protein engineering going on in the base editing field! From CRISPR-free...

13. Plasmids 101: Broad Host Range Plasmids

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    ...inducible promoters with different degrees of stringency and inducibility (Toh et al. 2023), and additional...across gram negative hosts. Biotechnology and Bioengineering, 106(2), 326–332. https://doi.org/10.1002/bit...

14. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

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    ...blog post). Optimizing FLP FRT through smart engineering Different FLP versions – making FLP efficient...Frank et al. 2003. “A Directional Strategy for Monitoring Cre-Mediated Recombination at the Cellular Level...

15. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

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    ...Vader in "Return of the Jedi" While Vader was referring to the evil side of a mystical "Force," this quote...posttranslational modifications can disrupt FP folding rendering the protein non-fluorescent. Unfortunately, accumulated...

16. Plasmids 101: Control Plasmids

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    ...the plasmid without the independent variable. Referring back to the experiment associated with Figure ...aliquot/preparation. The replicate control isn’t measuring or assessing reproducibility of the effect of ...

17. Plasmids 101: E. coli Strains for Protein Expression

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    ...high protein levels including:  ompT: Strains harboring this mutation are deficient in outer membrane ...recommended as these auxillary plasmids may be isolated during the prepping process. How does inducible expression...

18. March for Science

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    ...Diversity and inclusion in science, technology, engineering and mathematics (STEM) (17). This effort is two-fold... “politicized science” ABC News: White House considering gutting 38 EPA programs: draft budget  Science...

19. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

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    ...sequence detection method based on CRISPR-Cas12. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.02.29.971127... Detection of SARS-CoV-2 Genetic Material. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.04.20.052159...

20. Quick Guide to Near-Infrared Fluorescent Proteins

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    ...vivo probes, due to low autofluorescence, low scattering, and deep penetration of red-shifted light. The...about cellular events via  fluorescence changes occurring due to protein synthesis, degradation, or reconstitution...