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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,012 results for: RING

Showing: 761 - 780 of 1012 results

  1. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...their mental model of the protein. At some point during this interrogation process, they will almost invariably...recognize a PAM sequence, allow the complementary base pairing between the guide RNA and the target DNA strand...

  2. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...and protein turnover rates. Additionally, by engineering FP fusions associated with cellular organelles...Cas Plasmids - Protein Tagging. Beyond simply monitoring protein localization and activity, CRISPR tagging...

  3. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...mouse knockout lines should increase. New genome engineering tools also offer hope for the creation of new...knockout animals or cell lines, please consider sharing them by depositing with Addgene! Figures were ...

  6. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ...step of the series is consistent at 1:2. When preparing a dilution series, use a new pipette tip for each...components can affect the final readout. When preparing a standard curve, change pipette tips and mix ...

  7. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ...to get into the same cell. For simple cloning requiring just one or two recombination events, homemade...DNA polymerase This will minimize random errors during PCR: we recommend Phusion or Q5. Speed up your ...

  9. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    ...Neighborhood.   Work/life balance. Is it truly possible to bring all aspects of our lives under control? Balance ... friends. If there is any guilt, you should be sharing it absolutely equally, which means you only have...

  11. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ...biology because of an image that was honestly quite boring. My Bio 101 professor displayed a codon table —...mammalian cells like neurons, may not replicate during the course of your experiment, meaning you don’...

  12. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ... target guanosine instead, perhaps analogous engineering of UNG, which excises uracil, could allow it ...considerably. And there’s so much more exciting protein engineering going on in the base editing field! From CRISPR-free...

  13. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...inducible promoters with different degrees of stringency and inducibility (Toh et al. 2023), and additional...across gram negative hosts. Biotechnology and Bioengineering, 106(2), 326–332. https://doi.org/10.1002/bit...

  14. Hot Plasmids - August 2020

    Type
    Blog Post
    ...repressing and activating expression of TCF4 while monitoring transcript and protein levels. Plasmids for multiplexed...donor infection (CRISPR-READI) enables genome engineering using HDR donors lengths up to 4.9 kb. Find ...

  18. How to Write a Scientific Review Article

    Type
    Blog Post
    ...about. Instead of reading a paper and getting my bearings, I would read a paper, panic, and then download...think the field is going or what areas are worth exploring.  Edit and rewrite, then repeat If you’re like...

  19. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...mesothelioma. Asbestos, and certain other naturally occurring asbestiform minerals such as erionite, are the...an immediate stop to the use of asbestos, thus ensuring that future generations will be protected from...
Showing: 761 - 780 of 1012 results