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Addgene

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We narrowed to 1,006 results for: RING;

Showing: 761 - 780 of 1006 results

  2. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ...step of the series is consistent at 1:2. When preparing a dilution series, use a new pipette tip for each...components can affect the final readout. When preparing a standard curve, change pipette tips and mix ...

  3. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ...to get into the same cell. For simple cloning requiring just one or two recombination events, homemade...DNA polymerase This will minimize random errors during PCR: we recommend Phusion or Q5. Speed up your ...

  5. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    ...Neighborhood.   Work/life balance. Is it truly possible to bring all aspects of our lives under control? Balance ... friends. If there is any guilt, you should be sharing it absolutely equally, which means you only have...

  6. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ...biology because of an image that was honestly quite boring. My Bio 101 professor displayed a codon table —...mammalian cells like neurons, may not replicate during the course of your experiment, meaning you don’...

  7. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ... target guanosine instead, perhaps analogous engineering of UNG, which excises uracil, could allow it ...considerably. And there’s so much more exciting protein engineering going on in the base editing field! From CRISPR-free...

  8. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...inducible promoters with different degrees of stringency and inducibility (Toh et al. 2023), and additional...across gram negative hosts. Biotechnology and Bioengineering, 106(2), 326–332. https://doi.org/10.1002/bit...

  9. How to Write a Scientific Review Article

    Type
    Blog Post
    ...about. Instead of reading a paper and getting my bearings, I would read a paper, panic, and then download...think the field is going or what areas are worth exploring.  Edit and rewrite, then repeat If you’re like...

  10. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...mesothelioma. Asbestos, and certain other naturally occurring asbestiform minerals such as erionite, are the...an immediate stop to the use of asbestos, thus ensuring that future generations will be protected from...

  11. Hot Plasmids - August 2020

    Type
    Blog Post
    ...repressing and activating expression of TCF4 while monitoring transcript and protein levels. Plasmids for multiplexed...donor infection (CRISPR-READI) enables genome engineering using HDR donors lengths up to 4.9 kb. Find ...

  16. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...the plasmid without the independent variable. Referring back to the experiment associated with Figure ...aliquot/preparation. The replicate control isn’t measuring or assessing reproducibility of the effect of ...

  17. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...high protein levels including:  ompT: Strains harboring this mutation are deficient in outer membrane ...recommended as these auxillary plasmids may be isolated during the prepping process. How does inducible expression...

  19. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...tribulations of the process, I’ve provided guidance and mentoring to several people that found my insights helpful...'s about how YOU will fit into a department and bring in grants. What kind of colleague will you be? Do...

  20. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ...donor, you can deliver a silent mutation in a neighboring nucleotide along with your desired edit. This...Concordet J-P. Evaluation of off-target and on-target scoring algorithms and integration into the guide RNA selection...
Showing: 761 - 780 of 1006 results