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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 997 results for: Ran;

Showing: 761 - 780 of 997 results

  1. Hot Plasmids - October 2022

    Type
    Blog Post
    ...voltage indicator: JEDI-2P  by: Brian O'Neill  The François St-Pierre lab and collaborators have successfully...photoactivation of HcKCR1 in mouse neurons. D) Membrane voltage traces of action potentials in a HcKCR1...

  2. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    ...from the DNA. These apurinic sites if in double stranded DNA are repaired by base excision repair, but ...but can lead to mutations in single stranded DNA undergoing replication.   DNase on the other hand can digest...

  7. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    ...programmed regulation of DNA replication and gene transcription during vertebrate embryogenesis. My interests...Science journalism programs at various universities range from week-long workshops to more in-depth master...

  8. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ...some proteins. Protein folding issues, steric hindrances, and destabilization can be brought about by ...DOI: 10.1038/521274a Arribere, J. A., et al., Translation readthrough mitigation. Nature. (2016) 534, 719...

  10. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ...brain science, my curiosity did not ultimately translate to a desire to do the research itself. Several...past and chatting with individuals whose research ranged from wildlife conservation to abortion law to poetry...

  11. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    ...  For example, while “PCR test” has recently transitioned from jargon to non-jargon in the context of ...number of characteristics. Common ones include age range, familiarity with the subject, shared interests,...

  13. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    ...mRFP-GFP-LC3 Tandem Fluorescent Protein, but following translation, this fusion protein is cleaved to generate one...phosphatidylethanolamine (PE) and recruited to autophagasomal membranes, which leads to its degradation by autophagy. ...

  14. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    ...non-specific binding). Historically, glutathione-S-transferase (GST) fusion proteins have been immobilized on...joyful that getting to the final step and the transformed bacteria refuse to grow. Eventual success was...

  16. Plasmid Cloning by PCR

    Type
    Blog Post
    ...chance for mis-priming, so you can use a pretty wide range of annealing temperatures, but you may need to increase...sequencing. DNA replication by PCR has error rates that range from roughly 1 per 500bp to roughly 1 per 10 million...

  17. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    ...used to generate inversions, deletions, and translocations. It also provides researchers with many different...Where Do I Find One? If you need help making a transition into a new job or maybe to an entirely new career...
Showing: 761 - 780 of 997 results