We narrowed to 1,045 results for: STI;

...this post. Adeno-associated viruses (AAVs) make fantastic gene delivery vehicles for episomal gene expression...delivered through transfection of naked DNA. Other investigators who had been working to develop high efficiency...Central PMCID: PMC5568673. 8. Howes, Rob, and Christine Schofield. "Genome engineering using adeno-associated...

...focused scanning libraries. PFunkel: Of prior existing comprehensive mutagenesis methods, PFunkel offers...). If so, the nicking mutagenesis protocol will still work if the orientation of the BbvCI site(s) of .... PubMed PMID: 20676978. 3. Wrenbeck, Emily E., Justin R. Klesmith, James A. Stapleton, Adebola Adeniran...

...2013). Since then, Spinach2 has been used to investigate intracellular viral DNA dynamics in neuronal ...developed into a split aptamer that can be used to investigate RNA assembly. The split aptamer is composed of... blog Learn more about aptamer fluorophores Investigate other fluorescent biosensors Find other uses ...

...By Justin Ng and Alyssa Cecchetelli Science is a constantly evolving and demanding field, requiring a...feature genetic, physiological, or developmental characteristics that make them ideal for studying in a laboratory...regenerate, the axolotl has several attractive characteristics that make them an ideal model organism; they...

...urotensin. The MTRIA sensors will be useful for investigating neuronal signaling and for engineering improved...better response kinetics and photochemical characteristics than previous NIR calcium reporters iGECI and... to the potassium concentrations in cells than existing sensors. Using genome mining and structure-guided...

...quantification). But what if you’ve got several interesting protein targets and you want to know if they ...fluorophores, then labeling with the other, you can still record the signal separately for each target.   ...your next multiplex IF experiment! If you have questions or any favorite tips and tricks, leave a comment...

... unbound by protein. These unbound regions are “sticky” and have the potential to bind nonspecifically...milk, bovine serum albumin or other proteins. The sticky areas on the membrane will bind to the proteins... thoroughly. Vendors will frequently include suggestions for working concentrations, blocking buffers,...

...non-homologous sequence or a series of single-nucleotide substitutions along a 1.5 kb tract, all without selectable...found that mating improved editing efficiency suggesting that sperm are more permissive to Cas9 editing...CRISPR plasmids page. Future perspectives It is interesting to note that recipients for the 2015 Breakthrough...

...tool for predicting CGBE editing outcomes and assisting sgRNA selection is available at http://www.sunlab.fun...CGBEs. What's next? Base transversion editing is still advancing rapidly. If it follows a similar path ... synthesized, templated sequence. And the story still isn’t over! Keep an eye out for our next post about...

... to test the selectivity of your antibody. But testing is not one size fits all. The approaches used will...found that it is often best to determine this by testing them. For intracellular targets, we usually test.... The specific approach will depend on the characteristics of the target and whether suitable cell types...

...-019-0193-0 Walton, R. T., Christie, K. A., Whittaker, M. N., & Kleinstiver, B. P. (2020). Unconstrained...variants to develop five new base editors with distinct PAM sequences, expanding the number of available...

...concepts. Each short video answers an Antibodies 101 question, like "what is an antibody?" or "what is an IgE...scicomm ♬ original sound - Addgene We'll be posting these animations regularly from now until May, ...

...decorating your lab for the upcoming wintertime festivities! Help us spread some cheer by snapping a photo...Christmas, Hanukkah, ugly sweaters, Flannel Fridays, Festivus— you name it, we want to see it.      All photos...

...upcoming wintertime festivities: Hanukkah, Christmas, ugly sweaters, Flannel Fridays, Festivus— you name it,...

...Experiment.” Whatever your antibody application question, the Antibody 101 eBook is a great place to start...reach out to [email protected] with any thoughts, questions, or feedback. Happy reading! ...

... continue to grow the ways we share science by hosting our first ever CRISPR Week on social media. #AddgeneCRISPRweek...like to see what you think so we’ll ask you some questions on Friday - share with us how you use CRISPR. ...

...ship to, first on a temporary and now on a more lasting basis.   Table 1: Popular Rabies and VSV-G Plasmids...license fee, will be required. If you have any questions on ordering plasmids, please feel free to email...

...the details. Chapter 3 - Answering interesting biological questions using microscopy. Chpater 4 - Research...

...Dear scientists using AAV, We hear your questions! How much virus should I inject? How long do I need... patterns, and more. If you have feedback or suggestions about the AAV Data Hub, we welcome you to share...

...for your TikTok or Instagram story, we also have stickers to help express your emotions (while you’re on...when you use your favorite one. If you have any suggestions for future GIFs, feel free to let us know on ...