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We narrowed to 1,043 results for: STI;

Showing: 761 - 780 of 1043 results

  1. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...2013). Since then, Spinach2 has been used to investigate intracellular viral DNA dynamics in neuronal ...developed into a split aptamer that can be used to investigate RNA assembly. The split aptamer is composed of... blog Learn more about aptamer fluorophores Investigate other fluorescent biosensors Find other uses ...

  2. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    ...focused scanning libraries. PFunkel: Of prior existing comprehensive mutagenesis methods, PFunkel offers...). If so, the nicking mutagenesis protocol will still work if the orientation of the BbvCI site(s) of .... PubMed PMID: 20676978. 3. Wrenbeck, Emily E., Justin R. Klesmith, James A. Stapleton, Adebola Adeniran...

  3. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...By Justin Ng and Alyssa Cecchetelli Science is a constantly evolving and demanding field, requiring a...feature genetic, physiological, or developmental characteristics that make them ideal for studying in a laboratory...regenerate, the axolotl has several attractive characteristics that make them an ideal model organism; they...

  4. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...urotensin. The MTRIA sensors will be useful for investigating neuronal signaling and for engineering improved...better response kinetics and photochemical characteristics than previous NIR calcium reporters iGECI and... to the potassium concentrations in cells than existing sensors. Using genome mining and structure-guided...

  5. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...quantification). But what if you’ve got several interesting protein targets and you want to know if they ...fluorophores, then labeling with the other, you can still record the signal separately for each target.   ...your next multiplex IF experiment! If you have questions or any favorite tips and tricks, leave a comment...

  6. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ... unbound by protein. These unbound regions are “sticky” and have the potential to bind nonspecifically...milk, bovine serum albumin or other proteins. The sticky areas on the membrane will bind to the proteins... thoroughly. Vendors will frequently include suggestions for working concentrations, blocking buffers,...

  7. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    ...non-homologous sequence or a series of single-nucleotide substitutions along a 1.5 kb tract, all without selectable...found that mating improved editing efficiency suggesting that sperm are more permissive to Cas9 editing...CRISPR plasmids page. Future perspectives It is interesting to note that recipients for the 2015 Breakthrough...

  8. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...tool for predicting CGBE editing outcomes and assisting sgRNA selection is available at http://www.sunlab.fun...CGBEs. What's next? Base transversion editing is still advancing rapidly. If it follows a similar path ... synthesized, templated sequence. And the story still isn’t over! Keep an eye out for our next post about...

  9. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ... to test the selectivity of your antibody. But testing is not one size fits all. The approaches used will...found that it is often best to determine this by testing them. For intracellular targets, we usually test.... The specific approach will depend on the characteristics of the target and whether suitable cell types...

  10. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...-019-0193-0 Walton, R. T., Christie, K. A., Whittaker, M. N., & Kleinstiver, B. P. (2020). Unconstrained...variants to develop five new base editors with distinct PAM sequences, expanding the number of available...

  11. Antibodies 101 Series on TikTok

    Type
    Blog Post
    ...concepts. Each short video answers an Antibodies 101 question, like "what is an antibody?" or "what is an IgE...scicomm ♬ original sound - Addgene We'll be posting these animations regularly from now until May, ...

  12. #DeckTheLab to Win a Tasty Winter Treat This Holiday Season

    Type
    Blog Post
    ...decorating your lab for the upcoming wintertime festivities! Help us spread some cheer by snapping a photo...Christmas, Hanukkah, ugly sweaters, Flannel Fridays, Festivus— you name it, we want to see it.      All photos...

  13. 'Tis the Season to #DeckTheLab

    Type
    Blog Post
    ...upcoming wintertime festivities: Hanukkah, Christmas, ugly sweaters, Flannel Fridays, Festivus— you name it,...

  15. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    ... continue to grow the ways we share science by hosting our first ever CRISPR Week on social media. #AddgeneCRISPRweek...like to see what you think so we’ll ask you some questions on Friday - share with us how you use CRISPR. ...

  18. Find and Share AAV Data with Addgene's New AAV Data Hub

    Type
    Blog Post
    ...Dear scientists using AAV, We hear your questions! How much virus should I inject? How long do I need... patterns, and more. If you have feedback or suggestions about the AAV Data Hub, we welcome you to share...

  19. Expanded Access to Rabies Plasmids

    Type
    Blog Post
    ...genus members to the specified international institution. Requesters will need to provide a brief description...Bureau of Industry and Security. If you have any questions about this or other Addgene shipping and ordering...
Showing: 761 - 780 of 1043 results