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  1. Plasmids 101: Choosing an Antibiotic Resistance Gene

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    Blog Post
    ...therefore prevent cell death. One of the cool things about amp is that it's exclusively toxic to dividing cells...antibiotic selection. If you feel like multiple of the above antibiotics would work for you, they likely do! ...

  2. AAVs CREATed for Gene Delivery to the CNS and PNS

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    Blog Post
    ...or the PNS, respectively.  Read on to learn more about these two new AAV capsids and how they can be useful...Important considerations when using AAVs Learn more about using AAV as a tool for gene expression in mammals...

  3. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

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    Blog Post
    ...and detergents.  Organic Solvents  As mentioned above, organic solvents can be used to simultaneously ...permeabilizing with saponin. However, if you are not worried about washing away your target (for example, some cytoskeletal...

  5. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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    Blog Post
    ... activity, and much more. Read on to learn more about GFP, how scientists have evolved this versatile ...effects of alterations to this activity. Learn more about optogenetics at OpenOptogenetics and find optogeneic...

  7. Tips for CRISPR Gene Editing in Mice

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    Blog Post
    ...the pX330 plasmids used in the validation assay (above) and it is just as effective (Mashiko et al., 2014...Engineering with CRISPR Validate Your Genome Edit Learn about Non Homologous End Joining and Homology Directed...

  8. Getting the Most from Your Lentiviral Transduction

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    Blog Post
    ...Lentiviruses are a powerful laboratory tool often employed to establish cell lines that stably express...Get Tips for Titering Your Lentivirus Learn All About Viral Vector Elements Learn How to Do Genome-Wide...

  9. Plasmids 101: Gateway Cloning

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    Blog Post
    ... expression systems. Keep reading to learn more about the Gateway cloning method and its advantages. An... on the Addgene Blog Try Out FastCloning Learn About Methylation and Restriction Enzymes Use Colony PCR...

  10. How to Be an Excellent Trainee

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    Blog Post
    ...individuals who are polite, appreciative, and clear about their needs. Remember, your bench mentor’s training...prepared and take training seriously, as reviewed above. Offer to help clean up and/or make necessary...

  11. CRISPR 101: Targeting RNA with Cas13a (C2c2)

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    Blog Post
    ...potential applications of Cas13a given what we know about its structure and function? For starters, Cas13a... Check Out Our CRISPR Featured Topic Page Learn about Anti-CRISPRs Multiplex Genome Editing with Cpf1 ...

  13. Deciphering the Mysteries of Behavior with Viral Vectors

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    Blog Post
    ...distant regions. They also don’t give any clues about the types of neurons involved or whether their involvement... Viral Vectors Resources on Addgene.org Learn about Addgene's viral service Find viral protocols and...

  17. Multiple Plasmids at a Low Price: Kits from Addgene

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    Blog Post
    ...PUF Assembly Kit Below you’ll find information about what these kits contain, how they can be used, and...encoded sensors enable real-time observation of metabolite production." Proceedings of the National Academy...

  20. Antibodies 101: Beyond Surface Labeling

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    Blog Post
    ..., you may want to look at other methods. In the above cases, a good alternative is using a two-protein... region of the primary antibody (Figure 1b). As above, the advantage of this system is increased flexibility...
Showing: 761 - 780 of 962 results