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We narrowed to 951 results for: cha

Showing: 761 - 780 of 951 results

  2. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    ...identification of DNA binding proteins, this method can characterize nucleic acid-nucleic acid interactions at a ...2017). This upregulates expression of IGF2, a characteristic of many human malignancies. Additionally, the...

  3. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...genomic sequence from one another) — by various mechanisms, only one of the two strands’ DNA counterparts...γ-Retrovirus Guide References Cullen, B. R. (1992). Mechanism of action of regulatory proteins encoded by complex...

  4. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...generation of the RNP complex.  While one option is to purchase the Cas9 protein and a gRNA from a commercial ...performed rapidly and, in many cases, with reduced chance for off-target effects. As the CRISPR field continues...

  5. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...occurring animal model, the RPE65−/− dog, was characterized and found to suffer from early and severe visual.... PubMed PMID: 29983335. 9. Day TP1, Byrne LC, Schaffer DV, Flannery JG, 2014. Advances in AAV vector ...

  6. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ...final readout. When preparing a standard curve, change pipette tips and mix thoroughly by inversion or...dilution series, use consistent dilution factors, change pipette tips regularly, and mix samples very well...

  7. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    ...college student searching couch cushions for spare change, desperate to extract a few more photons from a...Fluorescent Protein Fusions Request Plasmids from the Michael Davidson Collection Find Fluorescent Protein Kits...

  11. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...lentiviral and retroviral packaging vectors are not interchangeable. While these differences may seem subtle they...the generation used to package the virus does not change the generation of the transfer vector. Q5: What...

  12. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ...during cell division. They don’t simply rely on chance (or random diffusion) to make this happen.  There...centromere-based incompatibilities: Insights into the mechanism of incompatibility mediated by low-copy number...

  15. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ...My Bio 101 professor displayed a codon table — a chart every molecular biologist has seen before, showing...bacteria and eukaryotes. But be careful — antibiotic mechanisms vary! Aminoglycoside antibiotics also block protein...

  16. Viral Vectors 101: Pseudotyping

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    Blog Post
    ...have different levels of cytotoxicity and the mechanisms behind the cytotoxicity can vary. For example...differentially inactivated by nonspecific complement mechanisms in serum. Sometimes this is due to the cells ...

  17. Plasmids 101: Gateway Cloning

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    Blog Post
    ...destination vectors prior to recombination, and it is exchanged with the gene of interest during the BP or LR ...Multiple Hosts. Curr Protoc Protein Sci. 2003 Feb;Chapter 5:Unit 5.17. PubMed PMID:18429245. 3. Ptashne, ...

  18. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...Although different types of cancer have different challenges and obstacles to overcome, mesothelioma sufferers...of immunotherapy is the use of drugs (or other mechanisms) that work through body’s immune system to fight...

  19. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...suited for recombinant protein expression. Many challenges can arise when over-expressing a foreign protein...minutes before adding the inducer. Growth Media: Changing media is tricky, because there can be a trade-...

  20. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ... This test can be performed in two steps for challenging samples as in Two-Step-SHERLOCK or in a fast,...the immobilised target sequences. Upon binding, changes in fluorescence can be visualised. Another approach...
Showing: 761 - 780 of 951 results