We narrowed to 1,052 results for: fer

...dTomato) that promote interactions between the FPs interfere with native interactions, preventing proper localization...papers should be used with caution, since the difference between studies is substantial (e.g, the poor...conclude, we recommend that you try a couple of different fluorescent proteins when protein tagging is the...

...bispecific, meaning that each scFv can target a different antigen; binding of each can be either simultaneous... a scientist looking for that perfect fit!  References and Resources Additional resources on the Addgene...AddgeneBrowse our recombinant antibody plasmid collection References Grumezescu, A. M. (2018). Drug targeting and ...

...affect protein binding patterns. Hereditary Hyperferritinemia-Cataract Syndrome (HHCS), a disease characterized...the Iron Response Element (IRE) RNA motif of the ferritin light chain RNA transcript. IRE motifs are bound...The RaPID plasmids are available from Addgene! References Ramanathan, M., Majzoub, K., Rao, D.S., Neela...

...difficult for an individual lab to produce - Hybridomas offer an alternative and renewable source of monoclonal... their use. Each RANbody is detectable by two different tags: a reporter and an HA tag. The reporter comes... link to its sequence in the comments below. References Sanes, J.R., & Yamagata, M. (2018). Reporter-...

...overexpressed from a plasmid but overexpression may interfere with the system. Another method for studying protein...whether or not the tags influence behavior. A conference and a breakthrough! During my graduate studies...you can contact her at [email protected]. References 1. Dieterich, Daniela C., et al. "In situ visualization...

...mutations). Although the precise mutations were different than those found in eSpCas9, the effect on specificity...and/or therapeutic settings. Will combining the different mutations found in each of these great tools further...continued improvements in genome engineering! References 1. Slaymaker, Ian M., et al. "Rationally engineered...

...replicates would have different labels - i.e. they would stochastically arise from different cells throughout...development of combination therapies.         References 1. Hyo-eun, C. Bhang, et al. "Studying clonal...

...because it “causes recombination” (although later referred to as the “cyclization recombinase”), is a 38 ...same type, but are not cross-compatible. Using different lox site variants allows Cre to catalyze more ...cre-dependent techniques?  Let us know in the comments! References 1. Bacteriophage P1 site-specific recombination...

...their mutations corrected, and the T-cells were transferred back into the infants. Almost all patients have...population. These pre-existing antibodies can interfere with virus-cell interactions and seriously hinder... cure research and science communication.     References 1. Gaspar, H. B., S. Howe, and A. J. Thrasher...

...methods flood the cell with protein, which can both interfere with a cell’s normal function and result in a ...portal. The plasmids should be functional in many different human cell types, and while most of the genes ...Allen Institute website at alleninstitute.org. References 1. Roberts, Brock, et al. "Systematic gene tagging...

...updated in 2023 by Susanna Stroik.  References and Resources References Yuxuan Wu et al. "Correction of a...frequency isn’t high enough to make a physiological difference, then the treatment will not work in a meaningful...

...base or 4-base overhangs, and with a variety of different enzymes and cycling conditions (see Table 1). ... cycling 3-base  PaqCl®, 1x T4 DNA Ligase Buffer 37-16 °C cycling 4-base PaqCl, 1x NEBridge... and don’t forget to ask DAD for some help! References Potapov, V., Ong, J. L., Kucera, R. B., Langhorst...

...dividing up the essential components of a virus into different plasmids. For a retroviral system this looks like... like the genetic cargo in one plasmid (the transfer plasmid), the envelope (Env) gene on a separate plasmid...Env, Gag, and Pol genes are not encoded in the transfer plasmid, the component that physically integrates...

... tomato plants that inherited an edited gene to confer resistance to the herbicide chlorsulfuron (Homo...want a negative-going GEVI that can be used in different illumination regimes with fast activation kinetics... a YFP-tagged G protein and a NanoLuc® (Nluc) Luciferase-fused detector that specifically binds to active...

...Find the MagLOV plasmid here!   References and resources References Duret, G., Polali, S., Anderson,...just be an artifact and eliminate sources of interference, but the result persisted. (Even better, multiple...

...spinal tract, CST, spinal cord and cranial nerve afferents labelled with a AAV8 expressing eGFP under the...sample and captured the reflected light using a different objective lens oriented perpendicular to the plane...Find plasmids from the Tsoulfas lab here.     References: H. Siedentopf, and R,. Zsigmondy.  (1902) Ann...

... lines with compound autosomes are completely infertile when mated to wild fruit flies6. It's also worth...information about gene drives can be found in the referenced publications. Thank you to our guest blogger...information, visit www.sculptingevolution.org. References: Burt, A. Site-specific selfish genes as tools...

...explains it, her work has always been focused on gene transfer, from transgenes to plants, chloroplast to the...anymore. The kit contains sequences for several different fluorescent proteins. Visualizing fluorescence...for those who go to work in cancer biology.  References: A Golden Gate Modular Cloning Toolbox for Plants...

...the physical DNA samples, for when that's the preferred mode of receiving parts." "We have found an amazing...functions, which we know and love. Boolean is in reference to George Boole who in 1854 was thinking about...tell us readers, what do you want to make?   References: Jerome Bonnet et al. "Amplifying genetic logic...

...journal editorials, science social media, and conference workshops for years. That is, presumably, why...these fields, and seeing names like Feng Zhang, Jennifer Doudna, Karl Deisseroth, and Ed Boyden crop up...scientist who enjoys learning a little bit about many different things (part of the reason Addgene has been such...