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We narrowed to 1,046 results for: fer

Showing: 761 - 780 of 1046 results

  1. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...replicates would have different labels - i.e. they would stochastically arise from different cells throughout...development of combination therapies.         References 1. Hyo-eun, C. Bhang, et al. "Studying clonal...

  2. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...because it “causes recombination” (although later referred to as the “cyclization recombinase”), is a 38 ...same type, but are not cross-compatible. Using different lox site variants allows Cre to catalyze more ...cre-dependent techniques?  Let us know in the comments! References 1. Bacteriophage P1 site-specific recombination...

  3. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...methods flood the cell with protein, which can both interfere with a cell’s normal function and result in a ...portal. The plasmids should be functional in many different human cell types, and while most of the genes ...Allen Institute website at alleninstitute.org. References 1. Roberts, Brock, et al. "Systematic gene tagging...

  4. CRISPR in the Clinic

    Type
    Blog Post
    ...updated in 2023 by Susanna Stroik.  References and Resources References Yuxuan Wu et al. "Correction of a...frequency isn’t high enough to make a physiological difference, then the treatment will not work in a meaningful...

  5. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    ...base or 4-base overhangs, and with a variety of different enzymes and cycling conditions (see Table 1). ... cycling 3-base  PaqCl®, 1x T4 DNA Ligase Buffer 37-16 °C cycling 4-base PaqCl, 1x NEBridge... and don’t forget to ask DAD for some help! References Potapov, V., Ong, J. L., Kucera, R. B., Langhorst...

  6. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...dividing up the essential components of a virus into different plasmids. For a retroviral system this looks like... like the genetic cargo in one plasmid (the transfer plasmid), the envelope (Env) gene on a separate plasmid...Env, Gag, and Pol genes are not encoded in the transfer plasmid, the component that physically integrates...

  7. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...their mutations corrected, and the T-cells were transferred back into the infants. Almost all patients have...population. These pre-existing antibodies can interfere with virus-cell interactions and seriously hinder... cure research and science communication.     References 1. Gaspar, H. B., S. Howe, and A. J. Thrasher...

  8. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ... tomato plants that inherited an edited gene to confer resistance to the herbicide chlorsulfuron (Homo...want a negative-going GEVI that can be used in different illumination regimes with fast activation kinetics... a YFP-tagged G protein and a NanoLuc® (Nluc) Luciferase-fused detector that specifically binds to active...

  9. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...Find the MagLOV plasmid here!   References and resources References Duret, G., Polali, S., Anderson,...just be an artifact and eliminate sources of interference, but the result persisted. (Even better, multiple...

  10. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...spinal tract, CST, spinal cord and cranial nerve afferents labelled with a AAV8 expressing eGFP under the...sample and captured the reflected light using a different objective lens oriented perpendicular to the plane...Find plasmids from the Tsoulfas lab here.     References: H. Siedentopf, and R,. Zsigmondy.  (1902) Ann...

  11. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    ... lines with compound autosomes are completely infertile when mated to wild fruit flies6. It's also worth...information about gene drives can be found in the referenced publications. Thank you to our guest blogger...information, visit www.sculptingevolution.org. References: Burt, A. Site-specific selfish genes as tools...

  12. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    ...explains it, her work has always been focused on gene transfer, from transgenes to plants, chloroplast to the...anymore. The kit contains sequences for several different fluorescent proteins. Visualizing fluorescence...for those who go to work in cancer biology.  References: A Golden Gate Modular Cloning Toolbox for Plants...

  13. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    ...the physical DNA samples, for when that's the preferred mode of receiving parts." "We have found an amazing...functions, which we know and love. Boolean is in reference to George Boole who in 1854 was thinking about...tell us readers, what do you want to make?   References: Jerome Bonnet et al. "Amplifying genetic logic...

  14. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    ... adaptations of this system have been made in different fields of study. In addition to the identification...purification with an anti-Cas9 antibody. These different tag systems and affinity purification schemes ...please also see our interview with Dr. Fujii. References Campbell, Amy E., et al. "NuRD and CAF-1-mediated...

  15. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    ...journal editorials, science social media, and conference workshops for years. That is, presumably, why...these fields, and seeing names like Feng Zhang, Jennifer Doudna, Karl Deisseroth, and Ed Boyden crop up...scientist who enjoys learning a little bit about many different things (part of the reason Addgene has been such...

  16. Tips for arabidopsis transformation

    Type
    Blog Post
    ...plant pathogenic species that stably integrates transfer DNA (tDNA) into the genomes of the plants it attacks... (Nakagawa et al., 2007). Alternatively, if you prefer Golden Gate assembly, there are a series of plasmids...countless transgenic plants in the process.     References 1. Emami, S., Yee, M. and Dinneny, J. R. (2013...

  17. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    ... lipoic acid ligase, biotin ligase, phosphopantetheinyltransferase) – These enzymes catalyze the covalent... We continue to develop derivatives that have different spectral properties, exhibit high fluorogenicity...modalities. Follow him on twitter @rhodamine110.   References 1. Liu, Zhe, Luke D. Lavis, and Eric Betzig. ...

  18. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ...development, and more; our work touched on many different issues, and our program didn’t stay in one place...management, and program evaluation are highly transferable. Use your experience and your passion(s) to ...coach you. There are many grant consultants who offer grant training, mentoring and coaching services,...

  19. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...tracking experiments (Rizzo, 2009). With many different FPs that emit across the visible spectrum, researchers...antibodies. The “spaghetti monster” name is a reference to the deity of the social movement, the church... to the expanded use of smFPs in the future. References 1. Viswanathan, Sarada, et al. "High-performance...

  20. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...0-5%. This blog post covers a brief review of different basic BE technologies and their adaptation for...Nickase Cas9 (D10A) Deaminase PAM requirements Reference CBE SpCas9 rAPOBEC1 NGG 6 SpCas9 Target-AID...improvement in the laboratory of Yinong Yang at PSU.   References 1. Komor, Alexis C., et al. "Programmable editing...
Showing: 761 - 780 of 1046 results