We narrowed to 82 results for: total dna

...ddPCR) Historically, a quantitative DNA hybridization method (DNA dot blotting) had been used to titrate... as opposed to purified DNA. This may be mitigated by using purified viral DNA as the template instead...the category: ELISA...qPCR...Digital droplet PCR...DNA dot blot...Transduction assay...SDS-PAGE...Electron...concentration of viral genomes (by qPCR or other DNA quantification methods - see below), since each viral...genome-containing viral particles relative to the total number of viral capsids (which can include empty... digital PCR system for absolute quantitation of DNA copy number." Analytical chemistry 83.22 (2011): ...

...interactions between DNA and protein. Micrococcal nuclease digestion is used to shear the DNA in these assays... uses antibodies to capture protein:DNA complexes. After capture, the DNA is released from the complex...suitable for DNA:histone modifier interactions, which have strong binding between the protein and DNA. Some ...Alternatively, the samples can be normalized to a total protein measurement. Both methods will also serve...light. Shear the samples using sonication to break DNA up into fragments of 300-1000 bps in length. Note...IP section above. Reverse the crosslink, isolate DNA, and perform downstream analysis. Special considerations...gentler but results in non-random fragmentation of the DNA. Controls for capture methods Capture methods should...

...GFP tag and 1kb of homologous DNA flanking both sides (about 2.7kb total)—is inserted into a plasmid backbone... a donor plasmid that contains long stretches of DNA on either side of the fluorescent protein sequence...

...vectors - which rely on insertion of DNA by the NHEJ pathway during DNA repair of the break to allow for ...either introduces a ssDNA nick, a dsDNA break, or is engineered not to cleave DNA and instead activates...an experiment. For CRISPR work, this often means DNA cleavage at unanticipated sites. While off-target...genomic locus must often be proximal to a PAM site for DNA cutting to occur. The RNA sequence and PAM site confer...more. While such software typically only considers DNA sequence and doesn’t take into account chromatin ...only reduce off targets where nicking/cleaving of DNA occurs, not for deadCas activator/repressor experiments...generated near each other, this will result in a DNA double strand break (DSB), the event required for...

...thermocycling program. One key difference is the plasmid DNA must be released from the bacteria in order to serve...amount of sterile water.  Pick 3-10 colonies in total to test, depending on the number of background colonies...need to lyse the bacteria to release the plasmid DNA by either briefly boiling the sample before use or... of the junctions between the plasmid and insert DNA. Colony PCR will greatly reduce the number of clones...

...to express multiple gRNA from a single construct. DNA and gRNA are not to scale.   Let’s start with... different destination vectors depending on the total number of gRNAs you wish to clone, from 2-7. For...destination vectors are used depending upon the total number of gRNAs you wish to clone, so you don’t ...cytidine deaminase fusion with Cas9 with a uracil DNA glycosylase inhibitor with a degradation tag (LVA...

...independently searched for this unique sequence of DNA and both identified lymphocyte antigen 6 complex ...receptor for PHP.B by looking for a unique sequence of DNA that was present in mice whose CNS was transduced...expression, mRNA splicing, or protein coding regions. In total, they tested 13 strains, with seven strains identified...chromosome. Of the genes located in this stretch of DNA, Ly6a seemed the most likely candidate due to its...

...computer. Maps, sequences, & downloadable .gb & .dna files Once you’ve identified a plasmid that you want...where the enzymes will cut (in basepairs), and the total number of times they cut. Use the filters on the...

...landing sites on opposite DNA strands. When the prime editor releases the DNA, the two 3’ flaps containing...previously-reported technique for precisely integrating large DNA sequences into a genome (Anzalone et al., 2022). ...prime editing and twin prime editing can integrate DNA cargoes of 80–100 bp, PASSIGE can integrate cargoes...other, while the 5’ flaps containing the original DNA sequence anneal with each other. Excision of the ... replacement, integration and inversion of large DNA sequences with twin prime editing. Nature Biotechnology...epigenetic editor consisting of dCas9 fused to a DNA methyltransferase — that can drive lasting transcriptional...methyltransferase. The strategy is generalizable to a variety of DNA-targeting proteins, so the team created versions ...

...introducing double stranded breaks (DSBs) into genomic DNA in many cell types and often results in biallelic...homology-directed repair (HDR), a distinct cellular DNA repair pathway. Accomplishing this usually involves...involves simultaneously introducing a homologous DNA repair template, such as a sin­gle-stranded oligodeoxynucleotide...at all, such as when you are editing a non-coding DNA region? To this end we developed a technology called...hundred. From these two steps, you’ll be screening a total of a few hundred clones - Still better than 10.000...