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  2. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...the picture had a broken controller and would not spin. It was going to cost far more than it was worth...microcontroller and a motor controller and made it spin again!  We’ve also learned to be resourceful and...

  4. Viral Vectors 101: Producing Your rAAV

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    Blog Post
    ...) using a high-speed ultracentrifuge. During the spin, the virions will form a band due to their high ... https://doi.org/10.1089/hum.2010.107 Zen, Z., Espinoza, Y., Bleu, T., Sommer, J. M., & Wright, J. F. ...

  8. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...now available. In this post, we’ll review the inspiration behind ExM, how it works, and some of the limitations...improve label density (Mikhaylova, et al. 2015).  Despite these limitations, ExM is a valuable tool, especially...

  9. CRISPR Antimicrobials

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    Blog Post
    ...beta-lactam resistant population to the antibiotic, despite this resistance gene being encoded by a high-copy... system to target virulent S. aureus, a common hospital-borne infection. Two rounds of phagemid treatment...

  10. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...follow in the footsteps of Luxturna™. However, despite promising results in preclinical studies some potential...diseases such as hemophilia, muscular dystrophy, spinal muscular atrophy, neurodegenerative diseases and...

  12. Plasmids 101: Broad Host Range Plasmids

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    Blog Post
    ...spp., Aliivibrio salmonicida, Anabaena spp., Azospirillum braziliense, Azotobacter spp., Bordetella spp...spp., Rhizobium spp., Rhodopseudomonas spp., Rhodospirillum spp., Shewanella spp., Thiobacillus spp., Xanthomonas...

  13. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...researchers at the Wyss Institute for Biologically Inspired Engineering. CRISPR/Cas9 is an enormously plastic...researcher at the Wyss Institute for Biologically Inspired Engineering.     Alejandro (Alex) Chavez is a ...

  16. An Integrin Antibody Toolkit from IPI

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    Blog Post
    ...response, tissue repair, and kidney development. Despite their ubiquity and significance, these receptors...comes from IPI’s work with the Boston Children’s Hospital and Harvard-based lab of Tim Springer, the founder...

  18. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

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    Blog Post
    ..., so some of the cells will still appear green despite having active Cas9 (see tip 2). Pro-Tips 1. To ...about 1 month, which I’d say is pretty amazing. Despite the shifting landscape of research opportunities...

  19. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...relatives suffering from similar diseases. Others were inspired by the imminent threat of climate change to study...interesting questions and your excitement will in turn inspire the people around you. Be proactive Rather than...

  20. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...any neuroscience project or topic of interest. Despite its power and versatility, the technique is remarkably...science writing. Derek is also a pianist and an aspiring composer. View his blog drsimonsaysscience.org...

  21. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...converge on a set of standard tools. Additionally, aspiring genetic editors can now compare the advantages...strand that does not base pair with the guide RNA. Despite being called the "non-target strand" in CRISPR ...

  22. 'Tis the Season to #DeckTheLab

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    Blog Post
    ...Here at Addgene we’re getting into the holiday spirit by kicking off our annual #DeckTheLab contest. ...

  24. Deck the Lab 2022!

    Type
    Blog Post
    ... The lab has officially welcomed the Christmas spirit. It's just unfortunate that we had no choice but...

  26. Summer Fun at Addgene!

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    Blog Post
    ...around the corner. Here at Addgene, we’re always inspired by the new seasons, so we've made some big summer...

  27. Newly Updated AAV Data Hub!

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    Blog Post
    ...AAV9 is so strong that you can even see dendritic spines!  Curious? Watch our AAV Data Hub Challenge recording...

  28. Editor's Choice, July 2016

    Type
    Blog Post
    ...blogger James D. Fessenden at Brigham and Women's Hospital. This post describes how to target and use FRET...
Showing: 91 - 120 of 294 results