We narrowed to 755 results for: ELP

...scientific community for years to come. We Are Here to Help Despite these challenges, Addgene remains steadfast...constrained funding. Addgene is uniquely positioned to help research institutions maximize their increasingly...protocols remain freely accessible to all researchers, helping teams to be successful in their work despite limited...the scientific community. Let us know by emailing help@addgene.org. We look forward to hearing from you...

...some time thinking about which type of gradient can help answer your particular question before running one...Table 1 (at the end of the post) has a number of helpful suggestions.  Gels Gel issues usually come down... cold room or packing the box with ice packs can help keep the system cool, as can lower voltages.  If...buffer around, which works well and consistently, to help troubleshoot background issues. It’s a quick way...run your western, check out our handy Table 1 for help in deciding which troubleshooting steps to take....* Load less sample/lysate  We hope this helps you on your western blot journey. Best of luck and...

...called our customer service line or sent an email to help@addgene.org, you’ve likely talked to one of us! ... order.  Still have questions? Visit the Addgene Help Center If you have additional questions about our...process not addressed in this article, check out our Help Center article How do I pay by purchase order (PO...questions about the ordering process, the Addgene Help Center is full of information about how to deposit...

...community in 2024 In 2024, your trust in Addgene helped unlock unprecedented scientific collaboration. .... Building tomorrow’s science today Your voices helped shape our journey throughout the year. Through ...countless conversations both online and in-person, you helped us understand how Addgene can better serve the ...excited to continue to grow and innovate with your help and guidance. Every plasmid shared, every collaboration...

...experience, It was helpful to have Addgenies reassure me that someone would always be there to help me. As the...Addgene’s Marketing Intern this summer! With the help of Project Onramp, I was able to gain access to ...down the right path. The frequent Zoom meetings helped me communicate my ideas. I was able to express ...

...and viruses, from crossing into the brain. While helpful from a biological standpoint, it makes delivering...information about commonly used and emerging capsids to help you decide which ones may be suitable for your experiment...area of interest, and health. While it’s always helpful to start with a literature search to see what others... promoters and enhancers (Mich et al., 2021) can help restrict expression to your cell type of interest...just reduces, empty viral capsids in your prep can help reduce the risk of an immune response. Strain-specific...table.     Figure 2: This decision tree can help guide your choice on which systemic capsid to use...options for systemic capsids, but we hope this post helps you find the right one to test in your application...

...there's a chance it'll never work. If you help others out, they'll help you too; both with your research and... not a fluke.” 3. Engage in various projects and help colleagues with their work. “Your one project may...with this passion. “Love what you are doing!” It helps when the time comes to work with full force. George...

...genome engineering, and studying its structure can help us understand the parameters of CRISPR experimental...arginine-rich bridge helix connects the two lobes and helps recognize the guide RNA. Unlike the cleavage domains...the CTD, ready to check for PAM sequencines. This helps explain why Cas9 can’t locate PAM sites until after...correctly before HNH or RuvC can cleave DNA, which helps limit off-target activity. What next? It’s not actually...different uses. In all cases, structural biology can help you visualize the system you’re working with and...

...for Publication policy with two changes that will help us ensure that plasmids don’t get left behind in...plasmids available as soon as your paper is published helps maximize the potential impact of your work. Plus...and waiting for replies. This updated policy will help our teams have clear workflows and keep things moving...questions about depositing, check out our website, Help Center, or email us at [email protected]. ...

...like to help share useful new resources with the scientific community – particularly ones that help address...lines you are currently or planning to use for helpful information on the known features and relevant ...reference, and shared allele search preferences to help you identify proximal lines you care about. If you...

...techniques and tools that helped make my experience run smoothly and can help other teams successfully ...results or ask questions to your collaborators. This helps everyone feel included in the project and accelerates...members of your lab that you see in person, and helps improve your interpersonal relationships with people...

...now has a helpful graphic to introduce you to the plasmid class covered on that page. To help you find ...also give you high-quality educational resources to help you do better research. CRISPR is an incredibly ...research toolbox, as well as additional resources to help you Plan Your Experiment. #12DaysofCRISPR and blog...

...this in the future. Here’s a list of blog posts to help you tackle the challenges of DNA repair (with bonus...how a better understanding of Cas9 biophysics can help enhance HDR. CRISPR 101: Ribonucleoprotein (RNP)...genome and just stick to RNA? Cas13 may be able to help you do that! How to Design your gRNA for CRISPR ...had great things to say about CRISPOR, a tool to help you design, pick, and close guides for Cas9 and ...

...closely with TTOs to help protect our depositors’ intellectual property, and to help requesting institutions...you requested from Addgene...the plasmid that will help you run that last additional experiment requested...commercialization Additional resources on Addgene.org Addgene Help Center: Tech Transfer Technology Transfer Information...

... While we love academic research, we hope we can help all researchers achieve happiness in their careers...Scientists: Why and Where Do I Find One? If you need help making a transition into a new job or maybe to an...new career path, a career coach might be able to help you. Head Addgenie Joanne Kamens explains what career...engineering. 10. Plasmids 101: Control Plasmids Need help thinking about the types of controls you’ll need...

...has certainly noticed: This year, the BeHEARD (Helping Empower and Accelerate Research Discoveries) Award...and his colleagues at the Wistar Institute in Philadelphia, PA will put their award to work on a condition...different variants and strategies out there. This will help us acquire a lot of those plasmids so that we can...after periods of high activity. The new work will help them to understand how different mutations lead ...

...CRISPR, there are two major areas where software can help. The foremost handles the design of sgRNA and represents... Benchling. Utility functions: These functions help speed up the sgRNA design process by removing repetitive...repetitive tasks and/or by providing features to help with the post-design process such as primer and ...applying CRISPR technologies to new organisms and will help you whether you have a full assembly or not. It ...to re-evaluate all tools. Report your findings to help everyone else. CRISPR technology has reached into...

...).  Evaluating a drug’s immunogenicity in-vitro helps researchers understand a drug’s safety and efficacy...silico and in vitro assessment of immunogenicity can help evaluate risk of an antibody.  In silico prediction...Treating these cells with the therapeutic antibody can help us assess the effects of the drug on the actual ...in silico method of immunogenicity assessment can help in determining the T-cell epitopes that will likely...

...bench mentor or anyone else available to directly help you during your visit. Reach out to your bench mentor...comfortable initiating this conversation, your mentor can help get the ball rolling! If you are applying to a structured...Remember that your host is going out of their way to help you with your research and teach you new techniques...questions if you don’t understand something. Offer to help out wherever you can and don’t forget to express...

...atlas, including over 3,500 human TF isoforms, to help researchers understand this regulatory network. ...mechanisms, although the unique barcodes for each TF help streamline identification. While the Zhang lab controlled...overexpression programs on cellular processes. For help with library transformation and amplification, refer... yet available, just ask an Addgene scientist at help@addgene.org! References and Resources  References...

...these tips and resources collated by Addgenies to help you keep calm and science on. Practice self-care...mental break, while meditating right before bed will help you prepare for sleep. At Addgene, our mindfulness..., you can contribute data to the AAV Data Hub to help the research community. Set expectations for your...overwhelmed right now, but hopefully these tips are helpful to you no matter what you’re trying to accomplish...

...catalog number for identifying a reagent can be helpful, but it lacks context unless it links to a verified...sequence information, and more. The plasmid page also helps researchers find other publications that have used...Having IDs linked to these metadata and publications help to enable reproducibility of experiments. Additionally...services such as Identifiers.org and N2T.net. To help authors comply with journal policies, Addgene now...

...CasPEDIA, there is a classification system, CasID, to help facilitate direct comparison of different enzymes...relevant properties of Cas enzymes. That’s a lot of helpful information! To get you started, we’ll give a quick...for a given Cas (SpyCas9a) from CasPEDIA.   Helpful resources and information For some researchers,...delivery of Cas, the size of the protein would be helpful to know for viral packaging limitations. Maybe ...

...finding ways to intentionally set up your bench to help manage details. For example, setting up your pipette...pipette tips to match your plate layout can help you track samples as you add them. Moving your reagents...your left side to your right as you use them can help you remember what's been added and what hasn't. ... as you work, and the possibilities for small, helpful changes are endless.    Fig. 2: Blugene...

...cells and molecules are recruited by the antibody to help destroy and remove a pathogen. Different isotypes...system proteins, which can initiate a cascade that helps attack extracellular pathogens.  Transportation ...: IgG1, IgG2, IgG3, and IgG4. IgE IgE antibodies help protect against parasites but also play a role in...isotype heavy chain constant domains. This switching helps B cells adapt during the course of an immune response...

...a selection strategy is not enough on its own to help you find your desired plasmid. In many cases, you...a screen to your cloning strategy? A screen will help you more easily identify successful clones so you...restriction digests to analyze plasmids, which can help you visualize this process, or visit our restriction...just 1 kb of DNA, custom primers are especially helpful in verifying mutations that wouldn't be reached...

...who met every other Thursday for 25 years. They helped one another navigate career changes and overcome...about peer mentoring. They want to find mentors to help them develop their skills and to hold them accountable...brings a diversity of perspective. Sometimes it is helpful to find peers that share a similar direction or...advice. Attend a local networking event together and help each other practice meeting people – compete for...

...cloning experiment. Here are some tips that will help you with your cloning project, and hopefully obtain...possible). There are many softwares out there than can help you at this stage and that can be used to simulate...simulate in silico cloning. This will definitely help in understanding if your strategy will be successful...or human. There are several online tools that can help you with that, they can be open source (i.e. primer3...

...little overwhelming, but here is what I’ve found helpful for my interviews in industry. Prepare stories ...questions. Writing a rough outline of these stories helped me remember them for the interview.  Prepare a ... by looking at their LinkedIn profiles. This can help spark a more natural conversation while also demonstrating...can use notes you took during your interviews to help craft these thank you's. Interviewing remotely Remote...

...intern. As a member of the Quality Control team, I help make sure the plasmids that go out our doors are...considering my future path. Talking to Addgenies has helped me get a much better idea of what lies ahead. Thus...up for failure. Listening to Kenkel’s story has helped me realize that is not true at all. Racheal Komuhendo...gave her the balance she needed. It is incredibly helpful to me to know that you can make big changes in ...

...heard that getting into industry early would only help. Like any major life decision, the transition felt...that environment sooner rather than later can be helpful. Publishing: As a postdoc at AstraZeneca, you are...varies between companies. Like most jobs, it does help if you know someone who’s already in industry to...Both paths will give you myriad opportunities to help you with the next step in your career.   Many thanks...

...kinds of information on our plasmid pages? Well, to help make the most of these pages, we've written this...plasmid would be useful for their own research, and helps requestors use the plasmid in their own labs. We...additional resources at the bottom of the page that help you cite this publication in your own work: How ...plasmid pages, please don't hesitate to contact us at help@addgene.org Resources on the Addgene Blog Read...

...those steps and provide you with more details to help you plan and optimize your own protocols. Here we...Antibodies are large proteins that need a little help crossing cell membranes as they are not able to ...permeabilize non-selectively. This non-selectivity is helpful when trying to pass tough-to-permeabilize membranes...This article on fixation has some more examples to help choose which fixative to use!) Ultimately, the ...

...our scientific technical support at help@addgene.org and let us help.  Check back on Thursday for an inside... we’ve built a new and improved search engine to help make this task a faster, easier, and frustration-free...define for a simple search. These pages provide helpful information about the subject, link to related ...

... health and medical organizations are working to help educate the public about proper antibiotic usage...Importantly, native, non-pathogenic bacteria remain to help recolonize the niche, reducing the chance of an ...replication-incompetent phage particles using a helper system. These CRISPR antimicrobials successfully...Future research will show if this new strategy can help us win back ground in the fight against bacterial...

...function. Small ubiquitin-related modifier (SUMO) can help with folding and stabilization, as can maltose-binding...aggregated protein is not useful protein. MBP tags can help with solubility issues, but scientists may also ...that improve disulfide bond formation in order to help keep your protein soluble.  Tags for affinity and...described in the post, but have been categorized to help you better assess their function. More detailed ...

.... Sharing knowledge and tools is the best way to help propel research forward and have the biggest impact... continue to develop improved, reliable tools to help carry out that research. Organizations like the ... collection of highly specific AAV enhancers can help scientists around the world better understand the...the brain, how it functions, and how we can help keep it healthy. Thank you to Dr. Yoav Ben-Simon for ...

...trying to introduce a homozygous mutation, it’s helpful to know how many copies you are looking for. Secondly...untreated population. Software such as CRISPResso can help with the data analysis. If NGS is not an option,...prediction analyses like CRISPRitz or CRISPOR can help narrow down the list of sites where off target editing... at Addgene: Read our CRISPRGuide Get practical help from our CRISPR Protocols Find gRNA Design Tools...

... sequencing can be done, this technique can also help pinpoint the genes within a sequenced genome that...for 1 h at room temperature. After this, it is helpful to subject your membrane to several freeze/thaw...remaining live E. coli. At this point, it can really help to incubate the membranes overnight in 500 mM to...specifically bind your target organism, it can be helpful to spot this protein directly onto nitrocellulose...

..., the Antibodies 101 eBook is designed to be a helpful desktop reference. Each chapter brings together...cytometry experiment, “Chapter 7: Sorting” is there to help guide you through the process. If you’re confident.... And, as always, don’t hesitate to reach out to help@addgene.org with any thoughts, questions, or feedback...