We narrowed to 808 results for: Eng

...conversion tract length in Drosophila. Elliot et al. (1998) suggests mammalian cells' tract lengths seem much...up with all these questions, Dr. Le Cong and Dr. Feng Zhang developed a Google Forum to allow scientists... the growing PubMed article list, the Genome Engineering Using CRISPR/Cas Systems has expanded out of ...wildtype or double nickase for my CRISPR genome engineering experiments? A1: When assessing which nickase...nickase type to use for your CRISPR genome engineering experiments, consider that wildtype Cas9 with optimized...Zhang lab has found that if a CRISPR is used to engineer a locus, isolate single cell colonies, then genotype...insertions by Homologous Recombination (HR), what is the length of each homology arm needed? How should the double...

...: A systemic capsid is a capsid that has been engineered to have enhanced function after intravenous administration... post, we’ll discuss how systemic capsids are engineered, as well as highlight some considerations for...region-specific transgene expression. (a) Several engineered capsids with unique tropisms are now available... rodents and non-human primates (NHPs). These engineered capsids have increased transduction efficiency...generally compared to the AAV9 serotype or an engineered precursor of the systemic capsid in question ...better efficacy than AAV9 and/or its intermediate engineered precursor. Considering systemic capsids for your...determined by the capsid and promoter/enhancer strengths in the cell type of interest as well as the type...

...light-pulse at one wavelength and stay on until a second pulse of light, with a different wavelength, turns it ... for your experiments.  Wavelengths Most vectors have an optimal wavelength to use but can be activated...of other cellular functions. Photoswitches are engineered molecules that induce conformational changes ...exposure to light within a specific range of wavelengths. Excitatory channels allow positively charged...and inhibitory channels activated at different wavelengths of light (Vierock et al., 2021). Step-function.... There are many different opsin (natural and engineered proteins responsive to light in combination with...designed to be activated within a narrower band of wavelengths to allow multiple tools, such as biosensors, ...

...and then derive mice from these engineered ESCs. To do so, the engineered ESCs would be injected into developing... the cells in the adult mice derived from the engineered ESCs), and chimeric adults mated to produce completely...Mouse Modeling blog posts: Part 1: Genetically Engineered Mice and Part 2: Breeding and Crossing Mice. ... to CRISPR when he used this new technology to engineer mouse models (Wang et al., 2013, Yang et al., ... Advantages of using CRISPR for mouse genome engineering From the very start, generating mouse models ..., compared to conventional methods of genome engineering, CRISPR is much more efficient at generating ...Marraffini LA, Zhang F (2013) Multiplex Genome Engineering Using CRISPR/Cas Systems. Science 339:819–823...

... specific wavelengths of light to initiate signaling. When FRET donor excitation wavelengths overlap with...proteins in whole animals, light at or above 600 nm wavelengths should be used. Researchers often use bioluminescence...traditional luciferases emit at near optimal wavelengths (Fluc emits at 610 nm) and do not require external...fluor then emits light at a tissue-penetrating wavelength above 600 nm. LumiFluors Schaub, F.X., et al....that could be co-imaged with GFP using the same wavelength for excitation. The result was the cyan-excitable...This strategy was first applied using RLuc8, an engineered Renilla luciferase, to produce a series of FP-RLuc8...concentrations of biological molecules. FRET sensors face challenges of photobleaching, autofluorescence, and, in ...

...optogenetics.  Challenges of engineering channelrhodopsins There are three main challenges to engineering channelrhodopsins...overcome channelrhodopsin design challenges To overcome these challenges, the Arnold and Gradinaru Labs ...predict the channelrhodopsin’s photocurrent strength, wavelength sensitivity, and off-kinetics.   This model...channelrhodopsins with high-throughput proteins engineering methods, like directed evolution, because channelrhodopsins...functional and their measured photocurrent amplitude, wavelength sensitivity, and off-kinetics correlated with...lower multiplicity of infection (MOI) makes it challenging for ChR2(H134R) to function because it’s lower...2019) Machine learning-guided channelrhodopsin engineering enables minimally invasive optogenetics. Nat ...

...PMC6342934. Teng, Fei, et al. "Repurposing CRISPR-Cas12b for mammalian genome engineering." Cell discovery...of Cas9s isolated from different organisms and engineered Cas9 variants that were able to target different..., the Osamu Nureki Lab described a rationally engineered Cas9, SpCas9-NG, which required only the di-nucleotide...time of our first blog post on choosing a Cas9, the Feng Zhang Lab had described eSpCas9, while the Keith...different Cas9 variants using gRNAs of different lengths (Lee et al., 2018) and found that Sniper-Cas9 showed...sensitivity across a large number of loci and gRNA lengths. Recent alternatives to Cas9 While Cas9 remains...characterized by the Zhang (Strecker et al., 2019) and Li (Teng et al., 2018) Labs also shows promise for in vivo...

...Td-CBE   Engineered TadA domain for cytosine base editing Lam et al., 2023 CBE-T, CABE-T   Engineered TadA...preferences.  The Liu lab also used natural and engineered Cas9 variants to develop five new base editors...discussed next!), RNA adenine deaminases have been engineered and/or evolved to catalyze cytidine deamination...compared to ABEmax. In an alternative approach, engineering and directed evolution of the TadA domain has...scientists to replace entire sequences of varying lengths. Base editing publication highlights In the table... Resources References Chen, L., Zhu, B., Ru, G., Meng, H., Yan, Y., Hong, M., Zhang, D., Luan, C., Zhang...Y., Guan, Y., Siwko, S., … Li, D. (2023). Re-engineering the adenine deaminase TadA-8e for efficient and...

... you generate a growing colony of genetically engineered mice, what can you do? In this post, we’ll cover..., and how to properly control for genetically engineered mice in your experiment. As you’ve learned in...in Part 1, there are many types of genetically engineered mice: transgenic mice, knockin and knockout mice...generate a desired phenotype Instead, genetic engineering should be used to separately introduce each mutation...offspring that have a combination of the mutations engineered into each of their parents. Here’s how to cross...Whether your mouse colony consists of genetically engineered mice with a single mutation or strains that have...can be mitigated in different ways. Prior to engineering a transgenic strain, the promoter being used ...

...the Wyss Institute for Biologically Inspired Engineering. CRISPR/Cas9 technology has revolutionized the...the fields of molecular biology and bioengineering, as it has facilitated the development of a simple ...-competent) Cas9 interacting with either a full-length gRNA (20nt complementarity to target site) or truncated...complementarity to target site). When complexed with a full-length gRNA, native Cas9's two catalytic domains, HNH ...different perturbations. Consider, for instance, the challenge of attempting to simultaneously and selectively...of modulating gene expression (6). Altering the length of gRNAs targeting different sites of interest ...orthogonal Cas9 species. Within a single cell, full-length guides targeting one set of genetic loci can be...

...characterizing the strength a species absorbs or reflects light or radiation at a specific wavelength, and the ...light or electromagnetic radiation at another wavelength (Heinrichs, 2009). When a fluorophore absorbs...difference in emission results in a slightly longer wavelength emitted than was absorbed. This phenomenon is...known as the Stokes Shift, or the difference in wavelength between peak excitation spectrum and emission...differences in their excitation and emission wavelengths, which are pivotal in their application in biotechnology...Hayashi-Takanaka et al., 2014). Other dyes may prove challenging to stain intracellular compartments in in vivo...applications that involve ranges of pH, and significant lengths of time, the stability of fluorescence over time...

...requirements, and photon emission wavelengths. In addition, researchers have engineered a variety of improved luciferases... and engineered to be extra bright. Other luciferases have been modified to change the wavelength of light...gracilirostris) Engineered  462 19 Furimazine None Optional AkaLuc(derived from Photinus pyralis) Engineered   677...Cofactors/substrates Required Not required Signal strength Lower Higher Sensitivity Higher Lower Background...assays Available Well-established Natural and engineered luciferases Although firefly luciferase was the...Properties of luciferase enzymes Name Natural or engineered Peak emission (nm) Size (kDa) Substrate Cofactors...substrates and measure luciferase output at different wavelengths. Addgene provides empty backbones with the luciferase...

...Cas family of proteins has many useful genome engineering enzymes, each with their own unique features ... and quirks. Here, we will review the genome engineering abilities of the lesser-known Cas enzymes while... DNA targeting Cas enzymes Cas9 – The genome engineering workhorse You rarely hear the word ‘CRISPR’ without...hearing ‘Cas9’. The Cas9 nuclease is a genome engineering powerhouse, and for good reason. It is guided...strand break. Cas9 has been studied for genome engineering purposes far more than any other Cas family protein...making Cas9 an adaptable choice for many genome engineering endeavors. For more information on Cas9 variants...curious feature of this Cas is that once it is engaged by its target, it will non-specifically cut ssDNA...

...without the challenges typically occurring with a fluorescence-based approach. These challenges can include...Addgene! Measuring messengers: growth factors Growth factors present unique challenges for study. These ...in their natural context is one of the biggest challenges in biology. From tumor suppressors to growth ... activity. Together, these limitations create challenges for measurements needed to move discoveries from...(Laissue et al., 2017). HiBiT overcomes these challenges and enables precise quantification with with ...factors that utilize exosomal transport is quite challenging. Work by Shibata et al. (2023) used HiBiT to ...endothelial growth factor A (VEGF-A) is one of these challenging proteins. VEGF-A mediates angiogenesis through...

...reprogramming  Regulation of telomere length with TZAP Maintaining telomere length is important for cellular function...on regulating telomere length with TZAP Multiplexible plant genome engineering toolkit The Voytas Lab ... Segment on PhoCl LSSmCherry1 & RDSmCherry1: Engineering and directed evolution of new RFP variants The...were developed as part of a larger strategy to engineer improved red fluorescent proteins (RFPs) and, ... of base editing. Kim et al. used natural and engineered Cas9 variants to develop five new base editors...The Denchi lab investigated an aspect of telomere length regulation called telomere trimming and have proposed...ZBTB48) in regulating the upper-bound of telomere length. Telomere trimming can be assayed by looking for...

...has been a lot of engagement with the Athena SWAN Charter and it has really strengthened the network of ...Laboratory, where she often feels more like an engineer than a biologist. Their focus at the lab is on...overcome them, and why she spends some of her time engaging with others on Twitter, among other things. MoClo...fluorescent proteins. Visualizing fluorescence can be challenging in plants because of the auto-fluorescence of...similar resource for those involved in monocot engineering projects. The MoClo tools will work in any dicot...science, and many other topics. What’s the value of engaging about science in that way for you? Patron: I started...Gate Modular Cloning Toolbox for Plants. Carola Engler, Mark Youles, Ramona Gruetzner, Tim-Martin Ehnert...

...The emitted light is at a longer wavelength than the exciting wavelength. Thus, FPs are proteins with this...by a specific excitation wavelength. This means that the emission wavelength can change. In a few cases...FPs classified by the emission color (emission wavelength range) FPs are usually classified by emission...emission color as outlined below (or emission wavelenght range).  By mutating GFP, the variants blue FP (BFP...Infra-Red ≥ 670 nm In addition to emission wavelength range, there are other traits that need to be...named after George G. Stokes) is the shift in wavelength from excitation to emission. For most FPs, Stokes...sure their emission light does not overlap in wavelength. In many microscopes the filters are not narrow...

...Open Yeast Engineering Project (OYEP). Through this project, community members will engineer yeast to glow...that anyone can engineer yeast to do similar things. The focus is to make yeast engineering accessible to...are skilled with audiovisual media, software engineering, business consulting, mathematics, and more. ...and in his free time, he is pursuing a plant engineering project at BosLab. Once he optimizes plant cell...knowledge with the community by teaching a DIY plant engineering workshop. Community projects at BosLab Not all...became a member at BosLab because she wants to engineer microbes. We’ve ordered a few yeast plasmids from...diversity in different soil conditions/locations but to engage the local community (including local garden and...

...BioBuilderClub BioBuilderClub engages high school teams around the world who combine engineering approaches and scientific...are asking. Teachers are also asking, “How do I engage my students in real-world lab activities? I’m trying... Kuldell from MIT’s Department of Biological Engineering. Built primarily from her 20 years of college...classroom are characterized by intense student engagement and joy, rather than a perfect lesson plan or... boost interest and understanding around the engineering of biology. Participating schools are provided...BioBuilder inspires students and teachers to apply engineering principles to biological systems. Today, what...BioBuilder module allows students to explore how engineering fields rely on rapid prototyping to develop and...

...nuclease domain. Feng Zhang's group utilized the bi-lobed architecture of Cas9 to engineer a series of "split...memory One of the goals of synthetic biology is to engineer cells capable of recording permanent "memories...display and high throughput binding assays, to engineer photoswitchable dimers which show over a 50 fold...activating endogenous genes Moritoshi Sato's Lab has engineered CRISPR-Cas9 to create a photoactivatable transcription... or (4) multiple sgRNAs with Cas9 for genome engineering. The versatility and ease of generating complex...potential applications of CRISPR/Cas9 in genome-engineering. One particularly attractive application involves...genes involved in neurogenesis, which had proven challenging using the existing dCas9-VP64 activator. The ...

...editing (Feng Zhang Lab 2015)  Fluorescent Proteins Plasmids and Resources Reference Feng, S., Sekine...producing fluorescent signal. Image modified from Feng et al. 2017 under CC-BY license. Many split-FP...conditions. Shyu et al. Biotechniques. 2006 Chang-Deng Hu Green sfGFP Split super-folder GFP, our...fluorescent proteins for endogenous protein labeling. Feng et al. Nat Commun. 2017 Bo Huang mNeonGreen3 ...conditions. Shyu et al. Biotechniques. 2006 Chang-Deng Hu Venus Improved N-terminal fragment VN155(...signal-to-noise ratio. Kodama et al. Biotechniques. 2010 Chang-Deng Hu mVenus pET-BiFC contains both fragments of...fluorescent proteins for endogenous protein labeling. Feng et al. Nat Commun. 2017 Bo Huang sfCherry3 Improved...

...Addgene to advance their research. Louis Bengyella Dr. Bengyella’s work has focused on elucidating the molecular...our first two plasmid grant awardees: Drs Louis Bengyella and Kwabena O. Duedu, both from the University...in Ghana. Read on to learn more about doctors Bengyella and Duedu and how they plan to use plasmids from... microbial pathogens. This work has given Dr. Bengwella expertise in using bioinformatic and proteomic...associated with pathogenesis. In his future work. Dr. Bengyella would like to use the expertise he’s developed...development of uterine tumors. According to Dr. Bengyella, this class of tumors is “a major public health... Ghana. We’re excited to see what impact Dr. Bengyella’s work will have on women’s health in Ghana and...

...funding, bioethics in the age of CRISPR, and the challenges of science becoming increasingly politicized...., “The Professional Scientist,” addresses the challenges of succeeding in science if you’re anything other... course the rapidly changing world of genome engineering via CRISPR.  Though the individual topics are...ramifications. Readers unfamiliar with academia’s challenges with publication and funding may be dismayed ...The three chapters in this section describe the challenges of trusting science in a “fake news and post-...has played in these fields, and seeing names like Feng Zhang, Jennifer Doudna, Karl Deisseroth, and Ed ...describes the author’s thoughts on science’s biggest challenges over the coming years. It is arguably the most...

...editing efficiency, and target access were all challenges for these early base editors. Further innovations...tackle this problem, Benjamin Kleinstiver’s lab engineered new variants of S. pyogenes Cas9. While the wild-type...sequence of NGG (where N represents any base), their engineered variants are more permissive: SpG can target ...Kleinstiver lab also worked on the opposite project: engineering Cas9 variants with a preference for specific ... L. A., & Zhang, F. (2013). Multiplex genome engineering using CRISPR/Cas systems. Science (New York, ...Gene Editing to Treat a Rare Genetic Disease. New England Journal of Medicine, 392(22), 2235–2243. https:... A., Thuronyi, B. W., Wilson, C., Koblan, L. W., Zeng, J., Bauer, D. E., Doudna, J. A., & Liu, D. R. (...

... in a variety of cells and organisms. However, lengthy genetic payloads and insufficient transcription...assembled tRNA-gRNAs is placed downstream of a lengthy promoter, which is used to drive the expression...efficient MBE with such a strategy, the need for lengthy promoters, such as U6 or EF1α promoters, has limited...derived from human or plant source), by tuning the length of 5’ leader sequences of a given tRNA to achieve...either from human or plant sources) in both the strength as a promoter and the activity to release gRNAs...results of their MBE efforts, the Gao Lab also engineered a DAP array for prime editing, achieving up to...also enable more convenient multiplex genome engineering of crops or allow for sophisticated CRISPR screenings...

...photobiology: why use light? In synthetic biology and bioengineering, scientists seek to directly probe and build...problems; inexpensive tools such as LEDs can be engineered to deliver precise, consistent, and controllable.... Complicated patterns of light intensity and wavelength can be used to study dynamic processes. Conversely...Conversely, varied input patterns may also be used to engineer new pathways with sophisticated gene expression... of edge detection. Tabor et al. subsequently engineered a second photosensitive TCS activated by green...-friendly. They experimented with the promoter strength of various components, removing inducible promoters...could be made suitable for various scientific or engineering applications.   References Refactoring and optimization...

...Cas9 and Cas12a, creates challenges for in vivo delivery and cell engineering applications. CasMINI, a...gene perturbation The Penn State Protein Ladder Engineered miniature CRISPR-Cas system for mammalian genome...flexible, modular tool for labeling. Here, Liu et al. engineer a chimeric single chain variable fragment (scFv...gels and can be detected on Western blots via engineered immunoglobulin binding domains.  Santilli, R.T.... https://doi.org/10.1038/s41598-021-96051-x Engineered miniature CRISPR-Cas system for mammalian genome... Figure 4: Overview of CasMINI for genome engineering applications. Image from Xiaoshu, et. al. 2021...system opens up new possibilities in both cell engineering and gene therapy.  Xiaoshu X, et al. Molecular...

...technology since its initial applications for genome engineering in 2012.  Many of these advances have stemmed... of dCas9; and 2) CRY2FL-VP64, a fusion of full-length CRY2 and the transcriptional activator domain VP64...PAM and had similar targeting specificity as full-length Cas9 (flCas9). When triggered by blue light (470nm...light-dependent (Yu et al., 2020). To express the N-Cas9, Haifeng Ye’s lab used the bacterial phytochrome BphS which...employed a photoactive strategy which had been engineered from naturally occurring photoactive proteins... specific site on the Cas9, the group used an engineered pyrrolysyl tRNA/tRNA synthetase pair which would... Grimm D, Correia BE, Eils R, Niopek D (2018) Engineered anti-CRISPR proteins for optogenetic control ...

...has developed a miniature Cas system (CasMINI) engineered from the uncultivated archaea Cas12f (Un1Cas12f1... system. The CasMINI system was developed by engineering the Cas12f scaffold gRNA and making strategic...CRISPR editing with a hypercompact Cas12f1 and engineered guide RNAs Yong-Sam Kim’s lab also created a ...the gRNA scaffold through five rounds of gRNA engineering.  They did not modify the Cas12f1 enzyme. The...facilitate its packaging into virus-like particles. Feng Zhang’s lab adapted the native PEG10 RNA sequences...plasmid tools to optimize prime editing systems. Engineered pegRNAs improve prime editing efficiency In the...the 3’ end with a structured RNA motif. These engineered pegRNAs (epegRNAs) protect the RNA from degradation...

...while writing each section. Research challenge The Research Challenge motivates the request for application...creates strong, well-directed meaning and delivers strength to the sentence. Below are the typical sections...how you, the expert, will address the research challenge. Now is the time to make bold statements. While...solution is the best approach to the research challenge. Demonstrating expertise in the field and past...how your innovation will address a variety of challenges and impact the field - its encouraged to dream...time budget writer, you may find this section challenging because it’s hard to determine the exact value... differs from publication writing and may be challenging to master at first. Additionally, reach out to...

...proteins or TALE proteins, proteins had to be re-engineered for each gene, making wide-scale gene activation...gene activation: rather than requiring protein engineering for each loci, CRISPR/Cas systems only require...methods rely on fusion proteins while others re-engineer components of Cas systems themselves. SunTag, ...Mediator (SAM) Description SAM uses specially engineered sgRNAs to increase transcription. This is done... through creating a dCas9/VP64 fusion protein engineered with aptamers that bind to MS2 proteins. These...activation of bacterial gene expression using an engineered CRISPR-Cas system. Nucleic Acids Research 41:...Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Nature 517:583–588 . https...

...structure-guided engineering of the PAM-interacting domain of Cas9. After multiple rounds of engineering, they developed...the "perfect" guide sequence for your genome engineering experiment. With CRISPR entering the clinic for... Nureki lab generated SpCas9-NG, a rationally engineered Cas9 with NG PAM compatibility, as the name implies...R.A. et al. PAM-flexible genome editing with an engineered chimeric Cas9. Nat Commun 14, 6175 (2023). DOI...Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants. Science 368,290-296(2020...Plasmids available here)   Nishimasu, H., et al.,Engineered CRISPR-Cas9 nuclease with expanded targeting ...Requirement and Expanding Beyond Cas9 xCas9: Engineering a CRISPR Variant with PAM Flexibility History...

... Kucera, M.Sc. and Eric Cantor, Ph.D. from New England Biolabs. Golden gate assembly limitations Embraced... assemblies that enable biosynthetic pathway engineering and optimization. However, current best practices... reduced these limitations through the use of engineered Type IIS restriction enzymes and careful choice...redesign was guided by both advances in the re-engineering of the original BsaI-HF® Type IIS restriction...In conjunction with BsaI-HFv2 (NEB #R3733), re-engineered to provide improved Golden Gate performance, ... Kucera, M.Sc. and Eric Cantor, Ph.D. from New England Biolabs. Becky Kucera is a Principal Development...Development focusing on DNA cloning and assembly, New England Biolabs, Inc.         Eric Cantor is a molecular...

...excitation wavelength (Salomonnson et al. 2012). They can also be excited efficiently at wavelengths between...development of cell sorters together with the engineering of fluorescently labelled antibodies made it ... lymph nodes at different times after antigen challenge, scientists were able to track where GFP labelled...et al. 2016, Cavanagh et al. 2008)? Technical challenges of two photon microscopy Intravital imaging is... at steady state or under physio-pathological challenge. However technical specificities have to be taken... also important to finely tune the excitation wavelength as it has been shown that even a small incremental...incremental change of the excitation wavelength can significantly affect emission intensities from fluorescent...

...recombinant protein purification and metabolic engineering. If instead you want to build genetic circuits...biology MoClo applications, but there are more! Engineering disease resistance through gene stacking, increasing...localization signals, plant markers, and reporters (Engler et al., 2014). This kit is a great starter for ...vectors, several delivery systems which can be engineered into the TUs, and even viral gene silencing vectors...kit has a focus on transformation and genome engineering. This is a specialized system with parts including...confined to that arena! The Mammalian Toolkit was engineered specifically for the types of expression and ...Multiplex MoClo Toolkit for Extensive and Flexible Engineering of Saccharomyces Cerevisiae.” ACS Synthetic Biology...

...Ellis does research in synthetic biology and bioengineering including a recent effort to construct a synthetic... SynBio. Chapter 3: Synthetic biology as the engineering of biology at the level of DNA using modular,...in the Ellis lab including yeast and E. coli engineering, microbial cooperation, and antibiotic production...lectures synthetic biology for the Department of Bioengineering. He graduated from Oxford University and then...supervision of Prof Jim Collins and specialising in engineering gene regulation in yeast. Dr. Ellis is now one...undergraduate projects. Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.      Additional...

...base editor when they realized that MPG could be engineered to act directly on unmodified G bases, resulting...Tong, Liu, et al., 2023). gGBE consists only of engineered MPG fused to nCas9. First, MPG removes the G ... target guanosine instead, perhaps analogous engineering of UNG, which excises uracil, could allow it ...considerably. And there’s so much more exciting protein engineering going on in the base editing field! From CRISPR-free...., Qu, C., Zhu, Y., Guan, Y., Wang, L., Liu, M., Feng, B., Song, G., … Li, D. (2024). Adenine transversion...deaminase-free base editors for G-to-Y conversion by engineered glycosylase. National Science Review, 10(8), ... T-to-S base editor and C-to-G base editor by engineered human uracil DNA glycosylase. Nature Communications...

...University, Jieze Zhang, an intern in the Yang Lab, and Sheng Yang, a professor in synthetic biology from the ...pEcCas/pEcgRNA system. This system works well for engineering single genome edits but the double strand breaks...interrupt genes (or gene clusters) in metabolic engineering. So far, we’ve used the MUCICAT system in two...compared to the industrial strain. In our metabolic engineering case study of N-acetylglucosamine biosynthesis... systems to choose from. Ready for metabolic engineering with improved cargo dedicated to gene (cluster... optimizing strains with low efficiency.       Sheng Yang obtained his Ph.D. from Shanghai Institute ... phenotypes and to design a new generation of engineered cells for green chemistry and biomedical applications...

...BioRender.com. How does it work? First, an engineered prime editing guide RNA (pegRNA) that both specifies... site and contains the desired edit (or edits) engages the prime editor protein. The prime editor consists...introduced, relies on the PE2 enzyme. PE4 and PE5: Engineering mismatch repair Even in PE3 and PE3b, prime editing...editing outcomes, including indels. Therefore, they engineered a dominant-negative mutant of the protein MLH1... RNA pseudoknots, calling this longer RNA an “engineered pegRNA” or epegRNA. Although the addition of ... which was made possible through their use of engineered virus-like particles. However, there’s still ...(2023). Phage-assisted evolution and protein engineering yield compact, efficient prime editors. Cell,...

... problem when the excitation wavelength is shifted to longer wavelengths. Therefore, the use of bright...binds a synthetic, fluorescent analogue have been engineered (Thorn, 2017). These fluorescent analogues are...28 kDa protein that resembles a cylinder with a length of 4.2 nm and a diameter of about 2.4 nm (Hink ...above. The smallest tags are peptides that are engineered to bind specifically and with high affinity to...., 2017) may improve detection. Since longer wavelengths have better tissue penetration, (infra)red fluorescent...usually dim relative to fluorescent probes, recent engineering efforts have increased the brightness of these...fluorescent proteins, biosensors, and optogenetic tools engineered from phytochromes." Chemical reviews 117.9 (2017...

... protect samples during cryo-EM grid freezing Engineered and evolved optimizations for prime editing CHARM...https://doi.org/10.1101/2024.02.06.579238.    Engineered and evolved optimizations for prime editing By...applications! Delivering prime editors (PEs) via engineered virus-like particles (eVLPs) offers an option...the donor sequence. The latest work evolves and engineers Bxb1 recombinase variants to optimize PASSIGE ..., P. Z., Palczewski, K., & Liu, D. R. (2024). Engineered virus-like particles for transient delivery of...recombinases and prime editing. Nature Biomedical Engineering, 10.1038/s41551-024-01227-1. doi:  https://doi.org... prion protein by AAV-mediated delivery of an engineered compact epigenetic editor. Science, 384(6703)...

...repair and genome engineering CRISPR-Cas9 has revolutionized the genome engineering world and made targeted...immediately applicable in DSB-induced genome engineering, it has biological importance for the repair ...CRISPR forum moderated by the Zhang lab CRISPR Challenges: Standardization and HDR CrispyCrunch Optimizing... (2018). Pubmed. CRISPR References: Genome engineering using the CRISPR-Cas9 system. Ran FA, et al. ...546; (2015). PubMed. Optimization of Genome Engineering Approaches with the CRISPR/Cas9 System. Li, K...PubMed. Making designer mutants in model organisms. Peng, Y. et al. Development 141; (2014). PubMed. Enhancing..., et al. Poster at Keystone Precision Genome Engineering. 2017.  Easi-CRISPR: a robust method for one-...

...theoretically, at least) fully and freely engage or disengage at will.  Informal education is education...typically ad hoc, for learners who can choose to engage or disengage with a very high level of independence, such...than average and are more likely to spend time engaging with science communication efforts.  Scientific...however, many others partner with formal learning to engage students at all levels.      Jargon is field-specific...space for people outside of the primary audience to engage if they so wish. (And let’s be honest: are you ...

...or intentional release of engineered organisms. “Is [the organism] engineered? Where did it come from? ...reached out to MIT labs in the Synthetic Biology Engineering Research Consortium. That is how Aditya Kunjapur...organism in the lab. Predicting lab-of-origin of engineered DNA This isn’t the only time researchers have...plasmid datasets) to predict the lab-of-origin of engineered DNA. Both of these groups have generated robust...complementary tools in a time where biological engineering and synthetic biology is expanding rapidly.  ...Deep learning to predict the lab-of-origin of engineered DNA." Nature communications 9.1 (2018): 3135....

...summarizes Zalatan JG, et al.'s recent paper, "Engineering Complex Synthetic Transcriptional Programs with...However, these approaches are often met with the challenges of regulatory bottlenecks and their scope is ... ‘biofactories’, most successes in metabolic engineering have been limited to the overexpression of various...host-independent, beyond the oft published metabolic engineering realm of S. cerevisiae and E. coli. The utility...scRNA design is readily apparent in metabolic engineering. The authors elegantly demonstrated the potential...By no means limited to the field of metabolic engineering, this is such a powerful and flexible technique... 1173-1183. (2013). PubMed. Zalatan, J. G. Engineering Complex Synthetic Transcriptional Programs with...

... versatility of the engineered SpCas9-NG and its variants can be used to engineer specific sites that ...Expanding the targeting range of CRISPRCas9 by engineering SpCas9 Article contributed by Shreya Vedantam...editing. PAM sequences can be from 2-6 base pairs in length and follow the DNA sequence targeted by the Cas9...PAM to recognize the target. Osamu Nureki’s lab engineered SpCas9 (SpCas9-NG) with relaxed preferences for...recognizes NG rather than NGG. In addition to the engineered SpCas9, they also fused the SpCas9-NG with the... promoters and enhancers, or have attempted to engineer loops to create genomic interactions that wouldn...

....  The question for her then was this: Can you engineer CRISPR-Cas9 activators that work well enough on...that they could be used by people with little bioengineering expertise? “This is the next step in high-throughput...Nature in December, Konermann and her colleagues in Feng Zhang’s lab describe just such a system, which they...Synergistic Activation Mediator or SAM. SAM is an engineered protein complex for the transcriptional activation..., unlike the mouse, we can’t rely on genetic engineering to optimize humans for CRISPRi and CRISPRa.” ...activating any human gene, go to http://sam.genome-engineering.org/. Crystal structure of Cas9 in complex with...Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Konermann S, Brigham MD,...

... explore their Local Engagement Playbook for step-by-step guides on how to engage in your local community...That’s what makes it so wonderful. The most challenging part of jumping into this career path from academia...ranges from astronomy to bioethics to chemical engineering and everything in between. Plus, there are many...folks is on Twitter- start an account and start engaging with the #scipol hashtag. Often there will be ...advocacy as a supplement to your career through engagement with your local or state governments. Most communities... your hair out, your successes, failures, and challenges you’ve overcome. This information may help you...completely new field without issue. This may be more challenging if you are trying to apply for an outright job...

...would work with our research team and software engineers to update our Lab Inventory Management System ...notice something: even though our scientists and engineers were attending the same meetings and hearing the...always knew from context what a “plate” was. Our engineers thought they knew.  As long as you have context...of “plates” (Fig. 1). When you have to tell an engineer that you want the LIMS software to create a barcode...meetings and 2) creating a pictorial guide for our engineers (Fig. 2). Creating this guide was relatively easy...not have to communicate with a team of software engineers in your lab, you likely will need to write protocols... convey the same concept to Morgan, our senior engineer? The answer to these questions--which were real...

...Sainsbury Lab website or Engler & Marillonet. Golden Gate and genome engineering In early 2011, the Bogdanove...within both the synthetic biology and genome engineering fields. We’ll walk you through how to apply this...ligation process is close to 100% efficient. Another strength of Golden Gate cloning is its scalability. Unique...overhangs to be efficiently assembled. Scientists can engineer unique enzyme recognition sites that flank their...cloning method with high throughput capability. Engler C, Kandzia R, Marillonnet S. PLoS One 2008;3(11...Epub 2011 Apr 14. PubMed.   Golden Gate cloning. Engler C, Marillonnet S. Methods Mol Biol. 2014;1116:119...