...possible, include positive and negative controls. Positive controls typically include cell lines or tissues...Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure...Towbin et al., 1979, Burnett et al., 1981).
Introduction to western blotting
In a western, proteins ...
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is the first step of a western. ...immobilized onto a membrane, usually composed of a nitrocellulose or polyvinylidene difluoride (PVDF) in a step...step called protein transfer. While both nitrocellulose and PVDF are commonly used, PVDF tends to be slightly...validated applications, pay close attention to the controls listed on the vendor’s website. Typically, the...
