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We narrowed to 438 results for: %s

Showing: 61 - 80 of 438 results

  1. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ...wondered if it could overcome some of Cas9’s shortcomings. Cpf1’s preferred PAM is 5’-TTN, differing from...nucleotide 5’ overhang 18-23 bases away from the PAM. Cpf1’s staggered cleavage pattern opens up the possibility...deletions at the 3’ end of the target sequence. Cpf1’s strength may lie in its low off-target editing rates...with SpCas9. Kleinstiver et al. suggest that AsCpf1’s off-target rate is similar to that of high fidelity...Brouns SJ, Charpentier E, Haft DH, Horvath P, Moineau S, Mojica FJ, Terns RM, Terns MP, White MF, Yakunin ...

  2. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...Ayoubi, R., Ryan, J., Biddle, M. S., Alshafie, W., Fotouhi, M., Bolivar, S. G., Ruiz Moleon, V., Eckmann,...https://doi.org/10.1038/nmeth.3995 Virk, H. S., Biddle, M. S., Smallwood, D. T., Weston, C. A., Castells.../doi.org/10.1111/bph.15467 Virk, H. S., Rekas, M. Z., Biddle, M. S., Wright, A. K. A., Sousa, J., Weston...RP91645. https://doi.org/10.7554/eLife.91645 Biddle, M. S., Alende, C., Fotouhi, M., Jones, C., Ayoubi, R., ... M., Healy, L. M., Robertson, J., & McPherson, P. S. (2019). Implementation of an antibody characterization...10.7554/eLife.48363 Uhlen, M., Bandrowski, A., Carr, S., Edwards, A., Ellenberg, J., Lundberg, E., Rimm, ..., Bowman, V. W., McCarthy, J., Amrani, Y., Duffy, S. M., Bradding, P., & Roach, K. M. (2021). TGFβ1 induces...

  3. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

    Type
    Blog Post
    ...another important step forward by dissecting that S. pyogenes protein into three parts. Their efforts ...protein, which they call SpyLigase (Spy comes from the “S” in Streptococcus and the “py” in pyogenes), capable... cell capture. Fierer et al (Proc Natl Acad Sci U S A. 2014 Mar 17. PubMed) Peptide tag forming a rapid...bacterial adhesin. Zakeri et al (Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):E690-7. Epub 2012 Feb 24. PubMed...

  4. Design Tips for Prime Editing

    Type
    Blog Post
    ...Nishimasu, H., Ran, F. A., Hsu, P. D., Konermann, S., Shehata, S. I., Dohmae, N., Ishitani, R., Zhang, F., &...Levesque, S., Jing, Y., Simpson, D., Zhao, A., Li, H., Yan, W., Goudy, L., Schmidt, R., Solley, S. C., Gilbert...Newby, G. A., Sahin, M., Osborn, M. J., Weissman, J. S., Adamson, B., & Liu, D. R. (2021). Enhanced prime....2021.09.018 Nelson, J. W., Randolph, P. B., Shen, S. P., Everette, K. A., Chen, P. J., Anzalone, A. V....

  5. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ... B. S., Purohit, A. M., Grippo, R. M., Gaykema, R. P., Spano, A. J., Beenhakker, M. P., Kucenas, S., Patel...References and resources References Duret, G., Polali, S., Anderson, E. D., Bell, A. M., Tzouanas, C. N., Avants...andrewgyork.github.io/gfp_magnetofluorescence. Karki, N., Vergish, S., & Zoltowski, B. D. (2021). Cryptochromes: Photochemical...Déjean, V., Dodson, C. A., Wallace, M. I., Mackenzie, S. R., Timmel, C. R., & Hore, P. J. (2016). Chemical...doi.org/10.1021/nl202950h.  Matsuoka, R., Kimura, S., Miura, T., Ikoma, T., & Kusamoto, T. (2023) Single-Molecule...

  6. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...K., Kim, Y., Lee, S., Min, S., Bae, J. Y., Choi, J. W., Park, J., Jung, D., Yoon, S., & Kim, H. H. (2019... Kim, H., Lalanne, J.-B., Nathans, J. F., Lee, D. S., & Shendure, J. (2024). Chromatin context-dependent..., Schep, R., Damodharan, T., Balázs, Z., Janjuha, S., Ioannidi, E. I., Böck, D., Steensel, B. van, Krauthammer... Weide, R. H., Morris, B., van Schaik, T., Manzo, S. G., Peric-Hupkes, D., van den Berg, J., Beijersbergen...

  7. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    ...spacer(s) into the CRISPR array Clone desired DNA cargo into the mini-transposon Transform plasmid(s) into...Dong F, Yang S, Yang J, Zhang Z, Qian Y, Chen J, Zhang J, Liu Y, Tao R, Jiang Y, Yang J, Yang S (2020) Multicopy...s41586-019-1323-z Peters JE, Makarova KS, Shmakov S, Koonin EV (2017) Recruitment of CRISPR-Cas systems...doi.org/10.1073/pnas.1709035114 Rubin BE, Diamond S, Cress BF, Crits-Christoph A,  He C , Xu M, Zhou Z...

  9. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    ...10.1038/s41589-020-0535-8 Takeda S, Kadowaki S, Haga T, Takaesu H, Mitaku S (2002) Identification of G protein-coupled... 950 different human genes encoding GPCRs (Takeda S et al., 2002) and many downstream complexes involved...

  10. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    ...Rohner, V., Kiss, C., Curreli, S., Dieter, A., Wilhelm, M., Harada, M., Duss, S. N., Dernic, J., Bhat, M. ... Z., Wu, H., Chen, W., Zhang, P., Zou, J., Hires, S. A., Zhu, J. J., Cui, G., Lin, D., Du, J., & Li, Y... Schütze, K., Moriwaki, Y., Heo, L., Ovchinnikov, S., & Steinegger, M. (2022). ColabFold: Making protein...

  12. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ..., C., Lian, C., Luo, S., Liu, L. D., Zhang, S., Zhang, Y., Wo, Y., Li, N., Wu, S., Gui, T., Wang, B., ....1038/nbt.4172 Kurt, I. C., Zhou, R., Iyer, S., Garcia, S. P., Miller, B. R., Langner, L. M., Grünewald...25217-y Zhao, D., Li, J., Li, S., Xin, X., Hu, M., Price, M. A., Rosser, S. J., Bi, C., & Zhang, X. (2021...Rajakumar, P. D., Prekop, H.-T., Chew, Y. T., Manivannan, S. N., & Chew, W. L. (2021). Programmable C:G to G:C...Replogle, J. M., Xu, A., Sisley, T. A., Weissman, J. S., Adamson, B., & Liu, D. R. (2021). Efficient C•G-...

  13. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...expression empty vector pFA6a vectors - Epitope tagging in S. pombe Insect/Baculovirus UAS, MT, Polyhedrin Targeted...expression pFA6a vectors - PCR-based C-terminal tagging in S. cerevisiae pCS2FLAG - N- or C-terminal ...display of proteins on the extracellular surface of S. cerevisiae cells His-tagged versions of pFastBac ... Plasmids page pBF3060 - Metabolic engineering in S. cerevisiae TALENs Gene targeting TALEN kits - Construct...capture and expression of bacterial gene clusters S. cerevisiae plasmids for multiple marker selection...

  14. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...., Alicea, B., Audisio, T. L., Auer, S., Bhardwaj, V., Burgess, S. J., Ferling, I., Gazda, M. A., Hoeppner...Ilangovan, V., Lo, H., Olson, M., Mohamed, S. Y., Sarabipour, S., Varma, A., Walavalkar, K., Wissink, E....Prepare and label a sample that expresses your target(s) at a high level, either naturally or by overexpression... D., Murray, K. D., Engebrecht, J., & Trimmer, J. S. (2019). A toolbox of IgG subclass-switched recombinant...10.17504/protocols.io.bs68nhhw Im, K., Mareninov, S., Diaz, M. F. P., & Yong, W. H. (2019). An Introduction...

  15. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    ...P, Sommella A, Iodice C, Cesi G, Simone S, Marrocco E, Rossi S, Giunti M, Palfi A, Farrar GJ, Polishchuk...Toriello E, de Simone S, Colella P, Iodice C, Polishchuk EV, Sommella A, Colecchi L, Rossi S, Simonelli F, Giunti...split vector approaches developed in the early 2000’s which allow researchers to deliver oversized cargo...and hematopoietic stem and progenitor cells (HSPC)s to ~40% expression in the K562 cell line (Bak et al....2009.280 Hirsch ML, Li C, Bellon I, Yin C, Chavala S, Pryadkina M, Richard I, Samulski RJ (2013) Oversized...

  16. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

    Type
    Blog Post
    ...-7_20  Karmakar, S., Panda, D., Panda, S., Dash, M., Saha, R., Das, P., Avinash, S., Shih, J., Yang, ...  References and Resources References Karmakar, S., Panda, D., Baig, M. J., & Molla, K. (2025). A unified...10.1038/s41587-019-0337-2  Molla, K. A., Sretenovic, S., Bansal, K. C., & Qi, Y. (2021). Precise plant genome...s41477-021-00991-1  Qiao, J., Zang, Y., Gao, Q., Liu, S., Zhang, X., Hu, W., Wang, Y., Han, C., Li, D., & ...

  17. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    ...Nogales presented the first detailed picture of Cas9's three-dimensional shape via x-ray crystallography,...Institute and MIT published another report showing Cas9's structural details. "Understanding this structure ...This Protein Could Change Biotech Forever."  David S. Weiss of Emory University summed it up well in that...

  18. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ...Chang, E. B., Ruscheweyh, H.-J., Sunagawa, S., Mclellan, S. L., Willis, A. D., Comstock, L. E., & Eren.... References and Resources References Demanèche, S., Bertolla, F., Buret, F., Nalin, R., Sailland, A.....67.8.3440-3444.2001 Fogarty, E. C., Schechter, M. S., Lolans, K., Sheahan, M. L., Veseli, I., Moore, R....2024.01.039 Garcillán-Barcia, M. P., Redondo-Salvo, S., & de la Cruz, F. (2023). Plasmid classifications...

  19. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes (PAM...

  20. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    ...Western Ontario. Adam summarizes Zalatan JG, et al.'s recent paper, "Engineering Complex Synthetic Transcriptional... the oft published metabolic engineering realm of S. cerevisiae and E. coli. The utility of simultaneous...potential of this to control a metabolic network in S. cerevisiae by using three RNA programs to differentially...him on Twitter @AdamChinFatt     References Qi, L. S. et al. Repurposing CRISPR as an RNA-guided platform...
Showing: 61 - 80 of 438 results