Skip to main content
Addgene
Showing: 61 - 80 of 185 results

  2. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...DNA. Finally, in 1953, Franklin along with James Watson and Francis Crick resolved the DNA double helix...:3870–3879 . https://doi.org/10.1093/nar/gkf495 Watson JD, Crick FHC (2003) Reprint: Molecular Structure...

  4. Cre-lox system

    Type
    Collection
    ... Natl Acad Sci. Jul 15;89(14):6232-6. PubMed . Matsuda T, Cepko CL. 2007. Controlled Expression of Transgenes...

  6. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...contains a nontargeting spacer flanked by two CRISPR repeats. The spacer can be digested using BsaI, allowing...were to develop an array using 3 distinct spacer-repeats, you could easily create 7 unique arrays (e.g. ...

  8. Weighing Reagents Protocol

    Type
    Protocol
    ...prior to handling the materials. Equipment Weighing boats or weighing paper Balance Scoopula or spatula Container...

  9. Gibson Assembly Protocol

    Type
    Protocol
    ...Link opens in a new window) OpenWetWare: Janet Matsen’s guide to Gibson Assembly (Link opens in a new ...

  12. 2022 Depositor Week

    Type
    Blog Post
    ...  Monday: Thank you post (this is it!)  Tuesday: Stats roundup on Twitter Wednesday: 2022 Blue Flame Depositors...

  14. CRISPR Cheat Sheet

    Type
    Blog Post
    ...Clustered Regularly Interspaced Short Palindromic Repeats: Sequences of DNA found in bacteria that allow ...

  17. Editor's Choice, August 2016

    Type
    Blog Post
    ...transitions at target sequences specified by gRNAs. Caveats, potential specificity issues, and other means ...
Showing: 61 - 80 of 185 results