We narrowed to 114 results for: CHL

1. Addgene's Tips for Plasmid Quality Control

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   Blog Post

   ...expected full plasmid sequences (examples include Benchling, Serial Cloner, and Snapgene), and save these ...

2. Working with Nuclear Receptors

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   ...reporter gene has over the years evolved from chloramphenicol acetyltransferase to luciferase, the basic ...

3. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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   ... apramycin, blasticidin, bleocin (zeocin), chloramphenicol, erythromicin, gentamicin, hygromycin, kanamycin...

4. Popular Retroviral Vectors and Their Uses in Scientific Research

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   ... Susanna Bachle...

5. Adenoviral Vector Production and Troubleshooting

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   ...standard method for purification of rAdV uses cesium chloride (CsCl) density gradients combined with ultracentrifugation...

6. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

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   ... Susanna Bachle...

7. Viral Vectors 101: Chemogenetics

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   ...Roth's and Takafumi Minamimoto's lab called deschloroclozapine (DCZ) shows promise as a CNO alternative ...

8. Cpf1 Update: Comparison to Cas9 and NgAgo

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   ...JS, Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev ...

9. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

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   ... Susanna Bachle...

10. Hot Plasmids: Winter 2025

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    Blog Post

    ... gene to confer resistance to the herbicide chlorsulfuron (Homo line 2) or wild type (WT), two weeks after...

11. Plasmids 101: Optimizing Plasmid Yields

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    Blog Post

    ...e.g. p15A, ColE1) can be increased by adding chloramphenicol to the culture medium. Improve the culture ...

12. Plasmids 101: Secondary Nanobody Toolbox

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    Blog Post

    ..., Chug, H., Görlich, D., Lee, C., Pleiner, T., Schliep, J.E., Stark, H., Trakhanov, S., & Urlaub, H. (...

13. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

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    Blog Post

    ...References and Resources References Ahlfen SV, Schlumpberger M. Effects of low A260/A230 ratios in RNA preparations...

14. Cpf1: A New Tool for CRISPR Genome Editing

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    ...Additional Resources Zhang lab Cpf1 blog post on Benchling ...

15. Transferable Skills Guide: External Collaborations

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    ... Susanna Bachle...

16. Save Time with Transient Plant Leaf Transformations

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    ...cannot be studied in hairy roots, as functional chloroplasts and leaf specific cell types are missing in ...

17. Twenty Years of Sharing: Addgene's Viral Vector Service

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    ...Cepko, C., Guerin, K. I., Rego, M. A., Ersing, I., Bachle, S. M., Kamens, J., & Fan, M. (2019). Adeno-Associated...

18. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

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    Blog Post

    ...Kaze, Christina Kellogg, Heather Martinez, Pat Schloss, Tara Smith, Marc Sze, Victoria McGovern, and Julie...

19. CRISPR-mediated Plant Base Editors

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    ...base editing. They first created a defective chloramphenicol resistance gene (CamR) by introducing a point...

20. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

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    ...the inner membrane space of mitochondria and chloroplasts, the extracellular milieu, and gram negative...