We narrowed to 116 results for: CHL
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TypeBlog Post...standard method for purification of rAdV uses cesium chloride (CsCl) density gradients combined with ultracentrifugation...
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Viral Vectors 101: Chemogenetics
TypeBlog Post...Roth's and Takafumi Minamimoto's lab called deschloroclozapine (DCZ) shows promise as a CNO alternative ... -
A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells
TypeBlog Post... Susanna Bachle... -
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet
TypeBlog Post... apramycin, blasticidin, bleocin (zeocin), chloramphenicol, erythromicin, gentamicin, hygromycin, kanamycin... -
Cpf1 Update: Comparison to Cas9 and NgAgo
TypeBlog Post...JS, Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev ... -
Hot Plasmids: Winter 2025
TypeBlog Post... gene to confer resistance to the herbicide chlorsulfuron (Homo line 2) or wild type (WT), two weeks after... -
Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles
TypeBlog Post... Susanna Bachle... -
Plasmids 101: Optimizing Plasmid Yields
TypeBlog Post...e.g. p15A, ColE1) can be increased by adding chloramphenicol to the culture medium. Improve the culture ... -
What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry
TypeBlog Post...References and Resources References Ahlfen SV, Schlumpberger M. Effects of low A260/A230 ratios in RNA preparations... -
Plasmids 101: Secondary Nanobody Toolbox
TypeBlog Post..., Chug, H., Görlich, D., Lee, C., Pleiner, T., Schliep, J.E., Stark, H., Trakhanov, S., & Urlaub, H. (... -
Cpf1: A New Tool for CRISPR Genome Editing
TypeBlog Post...Additional Resources Zhang lab Cpf1 blog post on Benchling ... -
Transferable Skills Guide: External Collaborations
TypeBlog Post... Susanna Bachle... -
Twenty Years of Sharing: Addgene's Viral Vector Service
TypeBlog Post...Cepko, C., Guerin, K. I., Rego, M. A., Ersing, I., Bachle, S. M., Kamens, J., & Fan, M. (2019). Adeno-Associated... -
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone
TypeBlog Post...Kaze, Christina Kellogg, Heather Martinez, Pat Schloss, Tara Smith, Marc Sze, Victoria McGovern, and Julie... -
Save Time with Transient Plant Leaf Transformations
TypeBlog Post...cannot be studied in hairy roots, as functional chloroplasts and leaf specific cell types are missing in ... -
CRISPR-mediated Plant Base Editors
TypeBlog Post...base editing. They first created a defective chloramphenicol resistance gene (CamR) by introducing a point... -
Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs
TypeBlog Post...the inner membrane space of mitochondria and chloroplasts, the extracellular milieu, and gram negative... -
Brain Initiative Collection
TypeCollection...AAV9 pAAV_hSynapsin_psychLight2 To generate Adeno-Associated viruses for expression of psychLight2 under... -
CRISPR Plasmids - Empty gRNA Vectors
TypeCollection...pyogenes Chloramphenicol Marraffini pCas9 42876 Bacteria BsaI yes, cut S. pyogenes Chloramphenicol Marraffini... -
CRISPR Guide
TypeCollection...alternatives Abudayyeh, O. O., Gootenberg, J. S., Essletzbichler, P., Han, S., Joung, J., Belanto, J. J., Verdine...Gootenberg, J. S., Abudayyeh, O. O., Lee, J. W., Essletzbichler, P., Dy, A. J., Joung, J., Verdine, V., Donghia..., O. O., Slaymaker, I. M., Makarova, K. S., Essletzbichler, P., Volz, S. E., Joung, J., Van Der Oost, ...