We narrowed to 253 results for: CIL
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TypeBlog Post... Protein Tags. Beyond E. coli: purifying from Bacillus subtilis and Pichia pastoris pCri also includes...other than E. coli, specifically the bacterium Bacillus subtilis and the yeast Pichia pastoris. The improved...
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Networking and Inspiring Young Scientists at Freshers Fairs
TypeBlog Post...Society was founded in 1944 with the goal of facilitating networking between chemistry students, especially...over 170 members in its first year. The society facilitates opportunities to learn about Synthetic Biology... -
Reagent Repositories Are Speeding up Science During the Pandemic
TypeBlog Post...dedicated facilities and manpower that a repository provides. Repositories around the world facilitate the ... -
Scientists Map the SARS-CoV-2-Human Interaction Network
TypeBlog Post...Additionally, they have an IRES-Puro marker to facilitate stable cell clone selection. Expression is driven...QBI UCSF. They are particularly interested in facilitating collaborative science, and making it accessible... -
R Bodies: Membrane-Rupturing Microscopic Tools
TypeBlog Post...apart; however, they still remain attached to the ciliate. It’s thought that they function as a kind of armor...Defensive extrusive ectosymbionts of Euplotidium (Ciliophora) that contain microtubule-like structures are... -
Management for Scientists: Delegating is Key
TypeBlog Post...develop the web page. The team manager must then facilitate appropriate communication between members of ...only way and may not even be the best way. This facilitates success and trust. Micromanaging is bad for everyone... -
Cre-Lox and Other Site-Specific Recombinases
TypeCollection... example, iCre or FLPo in mammalian cells may facilitate higher rates of Cre-driven recombination. Gene... -
Antibodies 101: Monoclonal Antibodies
TypeBlog Post...antibody-producing B cell and a myeloma cell (National Research Council (US) Committee on Methods of Producing Monoclonal...doi.org/10.1074/jbc.m116.736942 National Research Council (US) Committee on Methods of Producing Monoclonal... -
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs
TypeBlog Post... space of a standard pol III-gRNA, which will facilitate CRISPR/Cas9 multiplexing in systems with limited...overlapping activity. Multiple gRNAs can also facilitate deletions of other regions, such as noncoding... -
Plasmid Tools for Microbiome Studies
TypeBlog Post...detect expression of toxin mRNA from Clostridium difficile. As mRNA expression cannot be identified with ...infection, dysbiosis, and antibiotic treatment. To facilitate these studies, Pamela Silver’s lab developed ... -
What's New in CRISPR - November 2021
TypeBlog Post...utilizes a Cas12f from a different species, Acidibacillus sulfuroxidans, AsCas12f1. They found that this...protein PEG10 specifically binds its own mRNA to facilitate its packaging into virus-like particles. Feng... -
Addgene: The First Twenty Years
TypeBlog Post...to start an open science nonprofit that would facilitate the sharing of plasmids between scientists. The... r/labrats, Reddit. Part of that work meant facilitating the Material Transfer Agreement process, which... -
Engaging with science and society at pgEd
TypeBlog Post... broaden the conversations about genetics and facilitate dialogue between scientists and communities. ...With our focus on engaging the community and facilitating dialogue, a major part of our everyday work ... -
6 Tips for Grant Writing
TypeBlog Post... you will be doing your research in a group or facility that is a world leader in your chosen area of ...Learn How to Become a Grant Writing Professional Facilitate Career Planning and Personal Development with... -
Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux
TypeBlog Post...Future of Science, and the MIT Graduate Student Council will be held on Friday 16th and Saturday 17th September...Future of Science and the MIT Graduate Student Council, we shift our focus toward advocacy and action ... -
Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria
TypeBlog Post...bacterial integrative and conjugative elements in Bacillus subtilis (ICEBs1) system. ICEBs1 carries the genes...ICEBs1, an integrative and conjugative element in Bacillus subtilis." Plasmid 86 (2016): 14-25. PubMed PMID... -
Viral Vectors 101: Producing Your rAAV
TypeBlog Post...There are several facilities nationwide where you can obtain excellent quality, high titer rAAV (including...like adenovirus (Ad) or herpes simplex virus to facilitate efficient replication. The most common production... -
PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs
TypeBlog Post...most promising pegRNA designs and especially facilitates the use of prime editing in pooled settings with...models (such as PRIDICT/PRIDICT2.0) can greatly facilitate prime editing experiments, thus saving you time... -
Great Results Start with Great Standard Curves
TypeBlog Post...What do a viral vector production facility, food allergy testing lab, and the grad student down the hall...-to-day work. Indeed, viral vector production facilities frequently use qPCR with a standard curve to ... -
Lentiviral Vector Uses and Overview
TypeBlog Post...flanked by HIV-1 long terminal repeats (LTRs) that facilitate viral packaging and host genome integration. ...transcriptional activation from the 5’ LTR) and Rev (facilitates nuclear export of transcripts). A different viral...