...plasmids, or express a gene contained in a plasmid. During the propagation and/or transformation process, ...never receive a copy of the plasmid or will lose it during division. These non-plasmid-containing bacteria...while amp only affects the cell wall synthesis step during cell division. This is nifty because the recovery...it will induce mutations in the host/plasmid DNA during selection if it’s not completely bound up. While...

...defined chunks, called stories, and work on stories during short periods of time called sprints. The team ...different technical approaches that may come up during discussion.  One example of agility at work is ...we recently reduced the number of steps involved during checkout. Demo details for this would be something...developing new features and fixing bugs: we take time during each sprint to do some maintenance work on our ...

...magnetic beads and then incubated with a protein mix. During the incubation the immobilized antibody binds to...protein interactions, and limit high throughput procedures. However, agarose beads are often a more cost-effective...proteins from the sample prior to beginning the IP.  During the IP, the target protein in the sample binds ...weakly bound proteins in the sample are removed during a series of wash steps following the incubation...

...who referred to her lab as her family three times during a 10 minute conversation. Leaving a tight-knit ... “I’m a scientist.”    What should you expect during the lab → office transition? I’ve already provided...science,” and virtually every person you talked to during the day was also a scientist. But now you also ...upsetting, there are ways to get back to the bench during your off hours. DIY bio labs are cropping up in...

...generally segregate with the cell pellet during purification procedures. Even if you produce a large amount...for the entire translation process. For example, during high growth rate conditions, E. coli preferentially...biosynthetic enzymes may be preferentially charged during amino acid starvation thus resulting in higher ...

...time I’ve had a role in organizing collaborations. During my graduate studies at Karolinska Institutet I ...what was expected, and keeping track of speakers. During your graduate or postdoctoral work there are loads.... I am glad that I was able to build experience during my graduate studies in regard to reaching out to...Transferable Skills Guide Developing Transferable Skills During Science Training Resources on Addgene.org Check...

...structure determination LEA proteins protect samples during cryo-EM grid freezing Engineered and evolved optimizations...2024.01.21.576499.    LEA proteins protect fragile samples during cryo-EM grid plunge freezing By Rachel Leeson Cryo-EM...can be damaged at the air-water interface (AWI) during the freezing process, resulting in sample loss....-water interface protectant for fragile samples during cryo-EM grid plunge freezing. bioRxiv 2024.02.06.579238...

...cells with physical structure, separate chromosomes during mitosis, support transport of organelles and vesicles...can be critical when, say, the gene is essential during development and a knockout is not feasible or when...strategy to dissect the roles of three Rab proteins during a key stage of zebrafish development (Aljiboury... and Rab35 coordinate lumen and cilia formation during zebrafish left-right organizer development. PLoS...

...also ideal for signal amplification during these experiments. During detection, a biotinylated reporter...can be added to either terminus of a DNA sequence during a Polymerase Chain Reaction (PCR) by using a biotinylated.... Biotin can also be enzymatically incorporated during PCR using a biotin labelled dUTP. There are multiple...

... tends to be slightly more popular due to its durability and high binding capacity. In addition, PVDF ...blocking the membrane  from binding to the antibodies during the stain. The ideal blocking buffer depends on...common readout for subcellular fractionations, a procedure that isolates proteins from different cellular...polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National...

...agility as a nonprofit resource can prove to be vital during this period of constrained funding. Addgene is ...partner for archiving their biological materials during funding transitions. Enabling resource sharing:...what support would be most valuable from Addgene during this time. Your input will directly shape our response...

... contaminants that have been used during the purification procedure. Good indicators of DNA purity are...guanidine thiocyanate and guanidine hydrochloride used during plasmid extraction. Figure 2: Use cloning...

...of modified Ti plasmids during plant infection, as well as plasmid yield during preparation in E. coli....Agrobacterium to ensure that they are maintained during co-cultivation with the target plant species. Thus...

...were inspiring! We also got to hear more from them during a panel discussion with Dr. Craig Mello and Dr....enough to make up for the somewhat naïve questions. During the panel, an artist was sketching her interpretation...accelerate the launch of clinical applications. During the panel discussion, Dr. Mello took it upon himself...

...Wisconsin lab for a summer during my PhD and is responsible for my enduring love of cheese curds. We’...distribution service! Did you see a must-have plasmid during ASM? Let us know what’s missing in the collection...

... agar slices.  Molecular biologists used this procedure daily before the advent of techniques like PCR...temperature (thus also saving freezer space).  The procedure becomes less attractive when noting that one step... had to be careful not to stick your fingers in during a run or risk minor electrocution.  These days ...

...MCS/SacI site in pUCXKT, removing the stop codons during backbone digestion and providing the missing codons...utilizes the bulldozer-like quality of the ribosome during translation, which knocks off all mRNA binding ...both the GFP and the RFP proteins in the nucleus. During the first round of translation, the GFP proteins...exported to the cytoplasm. Furthermore, they find that during stress, mRNAs in P-bodies are translationally repressed...now follow movement of two or more targeted loci during the cell cycle or cell differentiation. This tool...observe intra- and post-Golgi segregation of cargo during their transport. Finally, the system can be adapted...

...are able... Thanks to everyone for being awesome during a tough time. We're all going to get through this...Addgene Team because they’re all doing amazing work during this difficult time. One function that rose to ...had to figure out a way to maintain our services during the crisis and maintain our workforce for when ...

...restriction enzymes or ligase, and requires no post-PCR procedures. Sounds easy right? The technique relies on the...insert, the magic of TOPO cloning happens. Basic procedure Let’s break down the steps needed for TOPO cloning...electro-competent E. coli - excess salt causes arcing during electroporation which would cause the electroporation...

...the gram negative bacteria’s thinner membrane. Procedure The stain is a pretty simple process. First, flood...photos/occbio/7092385559 For more specific procedural instructions, including timing, preparing the... them red or pink. At the end of the staining procedure, gram positive bacteria are stained purple or ...