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We narrowed to 443 results for: DUR

Showing: 61 - 80 of 443 results

  1. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ...magnetic beads and then incubated with a protein mix. During the incubation the immobilized antibody binds to...protein interactions, and limit high throughput procedures. However, agarose beads are often a more cost-effective...proteins from the sample prior to beginning the IP.  During the IP, the target protein in the sample binds ...weakly bound proteins in the sample are removed during a series of wash steps following the incubation...

  2. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    ...defined chunks, called stories, and work on stories during short periods of time called sprints. The team ...different technical approaches that may come up during discussion.  One example of agility at work is ...we recently reduced the number of steps involved during checkout. Demo details for this would be something...developing new features and fixing bugs: we take time during each sprint to do some maintenance work on our ...

  3. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...structure determination LEA proteins protect samples during cryo-EM grid freezing Engineered and evolved optimizations...2024.01.21.576499.    LEA proteins protect fragile samples during cryo-EM grid plunge freezing By Rachel Leeson Cryo-EM...can be damaged at the air-water interface (AWI) during the freezing process, resulting in sample loss....-water interface protectant for fragile samples during cryo-EM grid plunge freezing. bioRxiv 2024.02.06.579238...

  4. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...cells with physical structure, separate chromosomes during mitosis, support transport of organelles and vesicles...can be critical when, say, the gene is essential during development and a knockout is not feasible or when...strategy to dissect the roles of three Rab proteins during a key stage of zebrafish development (Aljiboury... and Rab35 coordinate lumen and cilia formation during zebrafish left-right organizer development. PLoS...

  5. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...also ideal for signal amplification during these experiments. During detection, a biotinylated reporter...can be added to either terminus of a DNA sequence during a Polymerase Chain Reaction (PCR) by using a biotinylated.... Biotin can also be enzymatically incorporated during PCR using a biotin labelled dUTP. There are multiple...

  6. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ... tends to be slightly more popular due to its durability and high binding capacity. In addition, PVDF ...blocking the membrane  from binding to the antibodies during the stain. The ideal blocking buffer depends on...common readout for subcellular fractionations, a procedure that isolates proteins from different cellular...polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National...

  7. Addgene Stands with the Scientific Community

    Type
    Blog Post
    ...agility as a nonprofit resource can prove to be vital during this period of constrained funding. Addgene is ...partner for archiving their biological materials during funding transitions. Enabling resource sharing:...what support would be most valuable from Addgene during this time. Your input will directly shape our response...

  9. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    ...of modified Ti plasmids during plant infection, as well as plasmid yield during preparation in E. coli....Agrobacterium to ensure that they are maintained during co-cultivation with the target plant species. Thus...

  10. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

    Type
    Blog Post
    ...were inspiring! We also got to hear more from them during a panel discussion with Dr. Craig Mello and Dr....enough to make up for the somewhat naïve questions. During the panel, an artist was sketching her interpretation...accelerate the launch of clinical applications. During the panel discussion, Dr. Mello took it upon himself...

  11. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...MCS/SacI site in pUCXKT, removing the stop codons during backbone digestion and providing the missing codons...utilizes the bulldozer-like quality of the ribosome during translation, which knocks off all mRNA binding ...both the GFP and the RFP proteins in the nucleus. During the first round of translation, the GFP proteins...exported to the cytoplasm. Furthermore, they find that during stress, mRNAs in P-bodies are translationally repressed...now follow movement of two or more targeted loci during the cell cycle or cell differentiation. This tool...observe intra- and post-Golgi segregation of cargo during their transport. Finally, the system can be adapted...

  12. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    ...Wisconsin lab for a summer during my PhD and is responsible for my enduring love of cheese curds. We’...distribution service! Did you see a must-have plasmid during ASM? Let us know what’s missing in the collection...

  13. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    ... agar slices.  Molecular biologists used this procedure daily before the advent of techniques like PCR...temperature (thus also saving freezer space).  The procedure becomes less attractive when noting that one step... had to be careful not to stick your fingers in during a run or risk minor electrocution.  These days ...

  14. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    ...are able... Thanks to everyone for being awesome during a tough time. We're all going to get through this...Addgene Team because they’re all doing amazing work during this difficult time. One function that rose to ...had to figure out a way to maintain our services during the crisis and maintain our workforce for when ...

  15. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...restriction enzymes or ligase, and requires no post-PCR procedures. Sounds easy right? The technique relies on the...insert, the magic of TOPO cloning happens. Basic procedure Let’s break down the steps needed for TOPO cloning...electro-competent E. coli - excess salt causes arcing during electroporation which would cause the electroporation...

  16. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ...a producer cell line like the widely used 293T. During vector production, assembled AAV vector particles...released from the producer cells into the supernatant during the production phase. However, this has not been...at the AAV vectors that are released from cells during production (Maguire et al., 2012). They saw that...

  17. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    ...the gram negative bacteria’s thinner membrane. Procedure The stain is a pretty simple process. First, flood...photos/occbio/7092385559 For more specific procedural instructions, including timing, preparing the... them red or pink. At the end of the staining procedure, gram positive bacteria are stained purple or ...

  18. Technical Design of a Western Blot

    Type
    Blog Post
    ...the lab. They can be stored at your bench during the procedure and at 4 °C when you’re not running a western...and reprobed multiple times. It doesn’t degrade during long-term storage and has both high binding capacity.... This is because proteins diffuse semiradially during the transfer, making membranes inherently “fuzzier...

  19. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

    Type
    Blog Post
    ...until it is triggered to enter the lytic cycle.   During both of these life cycles bacterial DNA can be ...in a wild type E. coli (Griffiths et al., 2000). During conjugation, genetic material is transferred from...Guillemin. "Multiple phases of competence occur during the Helicobacter pylori growth cycle." FEMS microbiology...

  20. Antibodies 101: Isotypes

    Type
    Blog Post
    ...each of the isotypes. IgM This isotype is produced during a primary immune response because all B cells begin...found in tissues. IgG is the predominant isotype during a secondary immune response, which is when the ...constant domains. This switching helps B cells adapt during the course of an immune response since it also ...
Showing: 61 - 80 of 443 results