We narrowed to 103 results for: EXO

1. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

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   ...-white screening require X-gal, an expensive exogenously-added substrate, chromoproteins don’t require...

2. Viral Vectors 101: Voltage Indicators

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   ...vivo or in vitro and a small molecule is added exogenously to measure a phenotypic response in the form ...

3. Plasmids 101: 5 factors to help you choose the right cloning method

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   ...ends. These fragments are then combined with an exonuclease, a polymerase, and a DNA ligase to carry out ...

4. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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   ...knockout, you might be happy targeting anywhere in an exon. But things get more complicated if you’d like to...

5. Fluorescent Proteins 101: Fluorescent Protein Timers

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   ...Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed...

6. PITChing MMEJ as an Alternative Route for Gene Editing

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   ...gRNA The gRNA should target near the last coding exon of your gene of interest. For ideal use, this gRNA...

7. Course-Based Undergraduate Research Experiences (CUREs)

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   ...Dustin, J., Poirier, V., Pellettieri, J. 2020. The exon junction complex is required for stem and progenitor...

8. CRISPR 101: Making a Knock-In Cell Line

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   ...endogenously available sister chromatid, though exogenous DNA can be used as well. Below are some considerations...

9. An Introduction to Adenovirus

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   ...surface proteins (especially fiber’s knob domain, and hexon), as well as other intracellularly-exposed proteins...

10. Viral Vectors 101: The Retroviral Lifecycle

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    ... membrane, and acquired as an envelope during exocytotic budding, as the viral particle emerges. Retrovirus...

11. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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    ...requires insertion of recognition sequences of an exogenous DNA-binding protein such as LexA into the target...

12. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

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    ...or alleles that was driving the PHP.B phenotype. Exome sequencing of related hybrid mice narrowed the search...

13. Hot Plasmids Spring 2024

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    ...and found the protein La (a small RNA-binding exonuclease protection factor) promotes editing (Yan, J et...

14. To Codon Optimize or Not: That is the Question

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    ...Synthesis NovoPro Labs Codon Optimization Tool (ExoOptimizer) Genewiz Codon Optimization  ATUM DNA2.0  IDT...

15. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

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    ...sequences to select from - in many cases several exons’ worth of DNA. Any ∼20 nucleotides of DNA can serve...

16. Hot Plasmids: Summer 2024

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    ...earlier system.  Rather than overexpressing an exogenous methyltransferase for epigenetic silencing, which...

17. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

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    ...likely a rate limiting step, but optimization of the exon-splitting site sequence can enhance the rate of ...

18. Quick Guide to Near-Infrared Fluorescent Proteins

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    ...transfected live HeLa cells with no supply of exogenous BV and after normalization to fluorescence of ...

19. Fluorescent Proteins 101: When GFP lets you down

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    ...hybrid between a genetically encoded tag and exogenously added components. For a review see Lotze et al...

20. Neurodegeneration Plasmid Collection

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    ...pHBS952 TARDBP-Exon1-ko-sgRNA1-SpCas9 TARDBP U6 ALS Rajat Rohatgi 107858 pHBS953 TARDBP-Exon2-ko-sgRNA2-SpCas9...Mulvihill 110486 Ultra-Exon1Q23-Myc-A HTT Myc UbC Huntington's Baoji Xu 110487 Ultra-Exon1Q145-Myc-A HTT Myc UbC... 110488 pUltra-Exon1Q23-Myc-B HTT Myc UbC Huntington's Baoji Xu 110489 pUltra-Exon1Q145-Myc-B HTT Myc ...EIF2AK2 SV40 VWM disease Petr Svoboda 121165 tau exon 9-12 splicing minigene MAPT CMV Parkinson's, FTD..._400 FUS Halo ALS Michael Ward 162274 HTT delta-exon1 HTT Flag CMV, P10 Huntington's Cheryl Arrowsmith...