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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 143 results for: Endog

Showing: 61 - 80 of 143 results

  1. Antibodies 101: Validation

    Type
    Blog Post
    ...that do not express your protein of interest endogenously and transfect them with a plasmid encoding your...ultimately planning to use your antibody to detect endogenous levels of the target in other cells or tissues...

  4. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...system described above, except that they use an endogenous nuclease system to cleave the gRNAs. gRNAs are...CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system.” Proceedings of the National...

  5. Zhang Lab CRISPR Page

    Type
    Collection
    ...complex for the transcriptional activation of endogenous genes. It consists of three components: A nucleolytically...sets of 3 vectors each available for mammalian endogenous gene activation using SAM: Addgene plasmids #61422...

  6. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system Plant Utilizes the tRNA... promoters. Bullock pCFD5 Drosophila Utilizes endogenous tRNA processing machinery to excise multiple ...

  7. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    ... day of CRISPR, Addgene gave to me: Selective Endogenous eNcapsidation for cellular Delivery (SEND). On...

  8. CRISPR Guide

    Type
    Guide
    ...Several labs have also controlled Acr activity with endogenous cell-type specific miRNAs to restrict CRISPR ...Validate genetic modification ). Browse Plasmids: Endogenous Tagging CRISPR Base Editing To overcome low HDR..., and the break can be repaired by the cell’s endogenous repair mechanisms. Figure 14: Overview of editing...repression gRNA G uide RNA , a synthetic fusion of the endogenous bacterial crRNA and tracrRNA that provides both...Jaenisch, R. (2013). Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional...Adamson, B. (2024). Improving prime editing with an endogenous small RNA-binding protein. Nature . 628 (8008...

  11. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...to gather the following types of data: Report endogenous DA release from a single dopaminergic fiber in...

  14. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ...separation of detection of a mutant protein vs. endogenous WT levels of the same protein when both are expressed...

  17. Hot Plasmids - January 2023

    Type
    Blog Post
    ...also demonstrated the ability to be used as an endogenous RNA imaging tool.   Fig. 1: Csm, shRNA...
Showing: 61 - 80 of 143 results