We narrowed to 146 results for: Endog

...that do not express your protein of interest endogenously and transfect them with a plasmid encoding your...ultimately planning to use your antibody to detect endogenous levels of the target in other cells or tissues...

...reaction. In fact, NLuc is bright enough to detect endogenous tagged genes generated through CRISPR/Cas9 knock-in...knock-in. Finally, NLuc is very inviting for endogenous protein tagging as it is only 19 kDa. An example...

...a molecular species with high affinity for an endogenous molecular target, meaning the dye will be attached...results in direct binding between the dye and the endogenous target, and thus no genetically encoded tag is...

...Additional Resources on the Addgene Blog Plasmids for endogenous gene tagging in human cells Plasmids 101: Protein...Protein Tags Fluorescent tagging of endogenous genes with SapTrap Resources on Addgene.org Browse CRISPR...

...system described above, except that they use an endogenous nuclease system to cleave the gRNAs. gRNAs are...CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system.” Proceedings of the National...

...complex for the transcriptional activation of endogenous genes. It consists of three components: A nucleolytically...sets of 3 vectors each available for mammalian endogenous gene activation using SAM: Addgene plasmids #61422...

... day of CRISPR, Addgene gave to me: Selective Endogenous eNcapsidation for cellular Delivery (SEND). On...

...plasmids can be used to create cell lines with endogenously-tagged gene variants or to differentiate human...

...Plasmids 101 Series. Get the Inntag Plasmids Tag endogenous proteins with CRISPR Fluorescently Tag Your Protein...

..., and the break can be repaired by the cell’s endogenous repair mechanisms. Cas3 belongs to the Class ...

...Controlling Stimulation ExogenousEasily controlled EndogenousNot easily controlled Unique advantages Mapping...

...to gather the following types of data: Report endogenous DA release from a single dopaminergic fiber in...

...organism’s genome (see figure) and to disrupt endogenous genes at the site of insertion. In both of these...

...separation of detection of a mutant protein vs. endogenous WT levels of the same protein when both are expressed...

...been to either systematically delete/overexpress endogenous genes or to introduce heterologous genes, but...

...allows for whole organism imaging. They created an endogenous ApoB-NanoLuc reporter by inserting the NanoLuc...

...useful for therapeutic applications in vivo where endogenous anti-AAV antibodies often neutralize the therapeutic...

...expression, the individual gRNAs are released by endogenous tRNA processing machineries, specifically, RNaseZ...

...cinch. This is because it doesn’t secrete its own endogenous proteins, making the recombinant protein its ...

...also demonstrated the ability to be used as an endogenous RNA imaging tool.   Fig. 1: Csm, shRNA...