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We narrowed to 20 results for: Endog

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Showing: 1 - 20 of 20 results

  1. Genetic Code Expansion

    Type
    Collection
    ...recognize endogenous tRNAs, and the orthogonal tRNA should not be an efficient substrate for endogenous aaRS...aminoacylate only the orthogonal tRNA, not endogenous ones, and endogenous aaRS enzymes should not aminoacylate...Jesse Rinehart 197655 B95(DE3) ΔA ΔfabR ΔserB 95 endogenous TAG codons mutated to TAA, RF1 (prfA) deleted... phosphoserine Ryan Mehl 218768 ATMW1 Removed endogenous EcTrpRS/ttRNA pair (knocked out trpS and trpT... Abhishek Chatterjee 218769 ATMW-BL21 Removed endogenous EcTrpRS/ttRNA pair (knocked out trpS and trpT...strain) Abhishek Chatterjee 218770 ATMY1 Removed endogenous EcTyrRS/tRNA pair (tyrS gene knocked out, tyrTV...strain) Abhishek Chatterjee 218771 ATMY3 Removed endogenous EcTyrRS/tRNA pair (tyrS gene knocked out, tyrTV...

  2. CRISPR Plasmids - Tagging

    Type
    Collection
    ...CRISPR plasmids for endogenous tagging of your gene of interest....system to add a tag (currently using FLAG) to endogenous proteins. This CRISPR/Cas plasmid-system consists... C- terminal affinity tag (3xFLAG-2xSTREP) on endogenous genes for the isolation of native protein complexes...of EGFP-2A-PuroR cassette to the C-terminus of endogenous proteins. The PITCh system depends on MMEJ (microhomology-mediated...simple and scalable CRISPR/Cas-based method to tag endogenous proteins by using donor constructs that harbor...

  3. Promega Plasmid Collection

    Type
    Collection
    ...luciferase that enables highly sensitive detection at endogenous expression levels. NanoLuc fusions are useful...applications where you want minimal disruption of endogenous protein expression and interactions, such as ...disturbing the virus of interest and monitoring endogenous protein dynamics in highly scalable assays. HaloTag...dynamic range can detect low-abundance proteins at endogenous levels in lysates or live-cells, thus preserving...

  4. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...Plasmids for Endogenous Gene Tagging in Human Cells on the Addgene Blog. Plasmids for Endogenous Gene Tagging...plasmids can be used to create cell lines with endogenously-tagged cellular structures. Addgene ID Plasmid...

  5. CRISPR Guide

    Type
    Collection
    ...Several labs have also controlled Acr activity with endogenous cell-type specific miRNAs to restrict CRISPR ... Browse Plasmids: Double-Strand Break (Cut) , Endogenous Tagging CRISPR Base Editing To overcome low HDR..., and the break can be repaired by the cell’s endogenous repair mechanisms. Figure 14: Overview of editing...repression gRNA G uide RNA , a synthetic fusion of the endogenous bacterial crRNA and tracrRNA that provides both...Jaenisch, R. (2013). Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional...Adamson, B. (2024). Improving prime editing with an endogenous small RNA-binding protein. Nature . 628 (8008...

  6. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...invader ( Figure 1 ). Figure 1: An overview of the endogenous Type II bacterial CRISPR system. Within the bacterial...allows us to create a variety of modifications in endogenous loci with unprecedented speed and specificity... Tag : Multiple methods make it easier to tag endogenous loci with epitope tags or fluorescent markers...

  7. Tetracycline Inducible Expression

    Type
    Collection
    .... To avoid secondary effects from repurposing endogenous systems that respond to natural stimuli like ...al., 2011), engineered to eliminate binding of endogenous mammalian transcription factors to further minimize...their sequence and eliminating interactions with endogenous factors. Experimental Considerations Expression...

  8. TALEN Engineering

    Type
    Collection
    ...-Fast and FLASH TALENs for Endogenous Zebrafish Genes TALENs for Endogenous Human Genes TALENs for the...

  9. Worm Expression Resources

    Type
    Collection
    ...Cas9, empty gRNA backbones, and constructs for endogenous tagging. Some examples of useful worm CRISPR ...Cas9 Editing Efficiency Fluorescent Tagging of Endogenous Genes with SapTrap Even more elegant: Single ...

  10. Neurodegeneration Research Collection

    Type
    Collection
    ...CNS myelination. (Lam et al. Nat Commun. 2022) Endogenously tag gene variants for Alzheimer's disease and...and Chemogenetics Plasmids . Find plasmids for endogenous gene tagging in human cells from the Allen Institute...

  11. Zhang Lab CRISPR Page

    Type
    Collection
    ...complex for the transcriptional activation of endogenous genes. It consists of three components: A nucleolytically...sets of 3 vectors each available for mammalian endogenous gene activation using SAM: Addgene plasmids #61422...

  13. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ..., and the break can be repaired by the cell’s endogenous repair mechanisms. Cas3 belongs to the Class ...

  14. Antibody Production

    Type
    Collection
    ... been retained. If the target antigen is not endogenously-expressed (e.g., using an anti-GFP antibody)...

  16. Zebrafish Plasmid Collection

    Type
    Collection
    ...encoding fluorescent proteins for tagging genes at endogenous genomic loci in zebrafish. NTR 2.0 - Jeff Mumm...

  18. Rett Syndrome

    Type
    Collection
    ...PI Mecp2 NLucTom Knock-in of NLuc-tdTomato at endogenous MECP2 locus Castaneus MECP2-NLuc-tdTomato mouse...

  19. Plasmids for Stem Cell Research

    Type
    Collection
    ...plasmids that can be used to create cell lines with endogenously-tagged gene variants or browse Addgene’s entire...
Showing: 1 - 20 of 20 results