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We narrowed to 93 results for: IRES-GFP

Showing: 61 - 80 of 93 results

  1. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    ...contains circularly permuted green fluorescent protein (GFP), and nLightR uses a red fluorescent protein, mApple..., Wu, Z., Wu, H., Chen, W., Zhang, P., Zou, J., Hires, S. A., Zhu, J. J., Cui, G., Lin, D., Du, J., & ...

  4. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ...or smaller. Some examples include a reporter like GFP, some smaller CRISPR-Cas proteins such as SaCas9,...this awesome experiment you want to do, but it requires some AAV. You’ve never worked with AAV before,...

  6. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    ...also have lentivirus for delivering control shRNAs, GFP, and rtTA from the Tet-on system. The first two can...Virus production can be a frustrating process that requires a large investment of time and resources for labs...

  7. Adenovirus Guide

    Type
    Guide
    ...pAdTrack series contains an IRES-GFP construct that enables co-expression of GFP with the transgene of interest...recombinant adenoviral vector production that contain IRES-GFP. Pseudotyping The production of viral vectors ...throughout viral vector production. During experiments, GFP can be used to sort cells infected with rAdV vectors...adenoviral vector production that do not contain GFP. Recombinant adenoviral vector (rAdV) Modified form... of genetic cargo. Production of HCAdV vectors requires using an additional adenoviral helper virus (HV...

  8. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...destination vector contains GFP, enabling you to select cells with high GFP expression. These cells have...editing, and a subsequent curing protocol that requires only 2-3 hours incubation. Kondo Lab multiplexed...

  9. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...ones? Many of the available biosensors use a single GFP or CFP/YFP-derived FRET pair. If you need to free...molecular neuroscience. 2014 Nov 18;7:88. Tian L, Hires SA, Looger LL. Imaging neuronal activity with genetically...

  10. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...Vectors Read about Reporter Gene like Luciferase and GFP Resources on Addgene.org Find Plasmids for Your...binding sites, commonly used in Drosophila Specific Requires the presence of Gal4 gene to activate promoter...Promoter from T7 bacteriophage Constitutive, but requires T7 RNA polymerase. When used for in vitro transcription... Negligible basal expression when not induced. Requires T7 RNA polymerase, which is also controlled by...Promoter from Sp6 bacteriophage Constitutive, but requires SP6 RNA polymerase. SP6 polymerase has a high ...Promoter from T3 bacteriophage Constitutive, but requires T3 RNA polymerase When used for in vitro transcription...

  11. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type
    Blog Post
    ...check out our article: Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection. Cellular... of hard-to-achieve factors. For example, FRET requires that fusion proteins be in close proximity and...FRET, as the overexpression of fusion proteins it requires can lead to aberrant subcellular localization ... construction Fusing FP fragments to your PIPs requires careful selection of FP fragments and, preferably...information, constructing appropriate controls may requires that you empirically test a number of mutants ...

  13. CRISPR Plasmids for Genomic Visualization

    Type
    Collection
    ... Cas9 (dCas9) fused to a fluorescent marker like GFP, researchers have turned dCas9 into a customizable...living cells. Visualizing a specific genomic locus requires recruitment of many copies of labeled proteins...gRNAs that tile the locus. Chromosome painting requires delivery of hundreds of gRNAs with target sites...

  14. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ...gene, while adding a GFP-tag (Figure 3). Achieving multiple modifications requires multiple recombination...to transformation (Figure 1). As this approach requires no special reagents or purification kits, it is...

  15. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ... a superfolder GFP fusion to stabilize the protein in mammalian cells. LwaCas13a-msfGFP can mediate cleavage... tracrRNA and crRNA to cleave target DNA (Cpf1 requires only a crRNA for target recognition and cleavage... PFS. This is in stark contrast to Cas9, which requires that each DNA target have high sequence identity...

  16. Bacterial Expression Systems

    Type
    Collection
    ...Vladislav Verkhusha 52732 52733 pET11a-link-NGFP pMRBAD-link-CGFP GFP (reconstructed) BiFC Lynne Regan 168257...Fluorescence (GFP+) Gram-negative bacteria Philip Poole 14460 pOT2 Promoter activity Fluorescence (GFPuv) Gram-negative... Guide to Selecting Fluorescent Dyes and Ligands GFP Fusion Proteins — Making the Right Connection Photoactivatable...pRsetB-his7-Perceval ATP:ADP ratio Fluorescence (GFP) Escherichia coli Gary Yellen 187836 pVoPo-01 Promoter...bacteria Philip Poole 14083 pAKgfplux1 Promoter activity Fluorescence (GFPmut3a) and luminescence (lux operon...bacteria Attila Karsi 14076 pAKgfp1 Promoter activity Fluorescence (GFPmut3a) Gram-negative bacteria Attila...-M ATP Fluorescence (GFPmut2) Escherichia coli Rahul Sarpeshkar 111614 pCdrA-gfpC Cyclic di-GMP Fluorescence...

  17. Genetic Code Expansion

    Type
    Collection
    ...first with a control reporter gene – GFP for E. coli or mCherry-GFP for mammalian cells. You should also...Mammalian TAG Peter Schultz 50831 pAcBac2.tR4-OMeYRS/GFP* tyrosyl-tRNA synthetase E. coli various unnatural...or DiZHSeC Mammalian TAG P. Chen 92047 pCOTS-pyl-GFP(35TAG) PylRS M. mazei Cyanobacterial TAG Lital Alfonta...analogs Mammalian TAG Huiwang Ai 160041 pRaGE Pyl TAG GFP Y35TAG PylRS M. mazei Bacterial TAG Lital Alfonta...C321.Ub-UAG-sfGFP all TAG sites changes to UAG, RF1 function removed, with Ubiquitin-UAG-sfGFP reporter ...182653 pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA Y306A/Y384F (AF) pyrrolysine (Pyl) tRNA...reporter George Church 98565 C321.ΔClpS.Ub-UAG-sfGFP all TAG sites changes to UAG, RF1 function removed, ClpS...

  18. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...markers were not increased compared to a control AAV-GFP. Not only did the AAV-SaCas9-sgRNA constructs mediate...drawbacks limit the utility of these systems: St1Cas9 requires a very specific PAM sequence that limits the number...

  19. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...aptamers able to bind to a small molecule derivative of GFP called 3,5-difluoro-4-hydroxybenzylidene imidazolinone...instability at 37°C (Ouellet et al., 2016) and requires a tRNA scaffold to improve its stability in vivo...

  20. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...fluorescent protein that could be co-imaged with GFP using the same wavelength for excitation. The result...for how sensitive you can make a reaction. NLuc requires 100-fold less protein to produce the same amount...detecting traditional luciferases’ dim light emissions requires long acquisition times that may be impractical...
Showing: 61 - 80 of 93 results