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We narrowed to 382 results for: Lor

Showing: 61 - 80 of 382 results

  1. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...the emission color (emission wavelength range) FPs are usually classified by emission color as outlined...familiarize yourself with the available FP emission colors and 10 points to keep in mind when choosing an ...Photoconvertible: These proteins can switch their color when activated by a specific excitation wavelength...Fluorescent Timers (FT): These proteins change their color over time. Therefore, these can be used as “timers...narrow enough to distinguish between closely related colors. Furthermore, most FPs  have a broad range of emission...proteins as biomarkers and Biosensors: Throwing color lights on molecular and cellular processes” Curr...

  2. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...only a single channel. In multi-color imaging experiments, the second color is usually provided by red fluorescent...of existing FP and epitope tags, researchers in Loren Looger’s lab have developed new molecular tags combining...antibodies, making them difficult to use in a multicolor/multichannel imaging set-up. In contrast, smFP...specific reactivities making them more useful for multicolor imaging experiments than standard FPs. The Looger...Looger Lab was thus able to easily perform a four-color labeling experiment using smFPs and standard neuronal...and detection: fluorescent protein structure and color variants." Cold Spring Harbor Protocols 2009.12 ...

  3. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...their lab coats handling glassware with brightly colored hazardous liquids. Working in a lab is nothing ...Research assistant Typically has a Master's or Bachelor's degree Generally a short-term position (1-2 years...biofuel production. There’s a lot out there worth exploring! Being excited about the science allows you to...and drive, but also makes for an experience more tailored to personal interests. Talk to people, ask questions... importance of building a network of mentors. Explore available resources! Many online resources and...Scientist While Poor” Keep an open mind as you explore opportunities The world of science is vast and...bench in academia or industry, so feel free to explore! Addgene has an entire page dedicated to different...

  4. Institute for Protein Innovation

    Type
    Collection
    ...anti-glypicans that accurately detect glypican presence and explore glypican function, providing a reliable and accessible...

  5. Optogenetics Plasmid Collection

    Type
    Collection
    ... cells. Other microbial opsins, such as the Halorhodopsins, Archaerhodopsins and Leptosphaeria rhodopsins...

  6. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type
    Blog Post
    ...Biology at the University of Colorado Anschutz Medical Campus in Aurora, Colorado, USA. The primary focus ...Biology at the University of Colorado Anschutz Medical Campus in Aurora, Colorado, USA.     Richard Gronostajski... of a common laboratory chemical, guanidine hydrochloride, as a potential treatment for a large number...be rescued by supplementation with guanidine hydrochloride (GdnHCl). Rescue occurs through guanidinium ...

  7. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...fluoresce one color but slowly mature until they predominantly fluoresce a second color. This change is...fluoresce red. The ratio of the mature color (red) to the immature color (green or blue) within a population... al. ""Fluorescent timer": protein that changes color with time." Science 290.5496 (2000): 1585-1588. ...et al. "Monomeric fluorescent timers that change color from blue to red report on cellular trafficking....

  8. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...General protein expression BL21 (DE3) pLysS* Chloramphenicol (pLysS) pLysS expresses T7 lysozyme to reduce...Expression of toxic proteins BL21 (DE3) pLysE* Chloramphenicol (pLysE) pLysE has higher T7 lysozyme expression...Expression of insoluble proteins  Rosetta2 (DE3)* Chloramphenicol (pRARE) Good for “universal” translation; contains...Expression of eukaryotic proteins Lemo21 (DE3)* Chloramphenicol (pLemo) Rhamnose-tunable T7 RNAP expression...T7 expression. The pLys plasmid contains a chloramphenicol resistance cassette for positive selection ... Julian Taylor-Parker...

  9. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...spectra. These probes can be combined in two-color and multicolor NIR imaging.  Small miRFPnano A separate.... They can also be used in crosstalk-free three-color imaging with green and red GFP-like FPs (Shcherbakova...Shcherbakova et al., 2016), as well as in four-color imaging (cyan, yellow, red, and near-infrared) (Qian et...monomeric near-infrared fluorescent proteins for multicolor imaging across scales. Nat Commun 11, 239 (2020..., V.V. Near-infrared fluorescent proteins for multicolor in vivo imaging. Nat Methods 10, 751-754 (2013...

  10. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...Chlamydomonas noctigama (CnChR1), Chloromonas subdivisa (CsChR), Chloromonas oogama (CoChR), and Scherffelia... mutagenesis or gene targeting Victor de Lorenzo's lab has engineered a modular mini-Tn5 vector that... proteins in the cephalochordate Branchiostoma floridae, named bfloGFPs. They went on to characterize ...fluorescent protein dsRed that changes irreversibly its color from green to red when it is oxidized. The Timer...have enabled the group to perform independent two-color optical excitation of neurons. The paper describes... in transgenic mice, the lab established a dual-color RNAi “sensor” mouse strain in which Cre expression...University previously changed the way researchers can explore important questions surrounding serine phosphorylation...

  11. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    ...opsin genes, and include bacteriorhodopsins, halorhodopsins, and channelrhodopsins, the first two found... excitation, whereas yellow light activates halorhodopsins to silence neuronal activity (Figure 1) (Wiegert...neuron activity on or off (Atasoy et al., 2008; Taylor et al., 2016; Hooper and Maguire, 2016). Recombinant...al., 2018; Deubner et al., 2019). For example, Taylor and colleagues used rAAV FLEx-based vectors to ...previously subjected to steady-state general anesthesia (Taylor et al., 2016). These very same neurons also play...PMID: 24700321. PubMed Central PMCID: PMC4100247. Taylor, Norman E., et al. "Optogenetic activation of dopamine...

  12. Finding Your Science Policy Path

    Type
    Blog Post
    ...Action Items:  Explore this career map to learn more where science policy roles exist. Explore these resources...diverse workforce. Out of all the career spaces I explored for my post-PhD life, the science policy community...Items:  Sign up to receive updates from ESAL and explore their Local Engagement Playbook for step-by-step...this is the most important question that you can explore. The answer will help you decide how to define ...the policy world much smoother.  Action Items:  Explore this comprehensive resource developed by Dr. Kelly...

  13. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    ... unexpected infiltrates the realm of focused exploration. Here, we delve into the importance of leaving...research lies in expanding the scope of your exploration. While it is essential to delve deeply into your...intersections of disciplines. Cast your net wider, explore tangential subjects, and let curiosity guide your... that embraces the unexpected and encourages exploration of tangents or seemingly unrelated ideas. It'... emerge when we allow ourselves the freedom to explore beyond the expected. By widening your search and...

  14. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ...they change color as they shift from "off" to "on" in the presence of calcium. This color change can be...addition to imaging neurons, Griesbeck adds that this color change has a particular advantage for observing ...applied this “trick” to the development of additional color variants. This resulted in the construction of redCALWY... donor and acceptor domains. He says, these new colors might now enable observation of the interaction...

  16. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    ...retained only in some species of bacteria. The stain colored one type, Streptococcus pneumoniae, while the alcohol...stain became known as gram positive, and the decolorized as gram negative. A German scientist, Carl Weigert...types. In water, the dye dissociates into CV+ and Chloride (Cl-) ions. When the negatively charged iodine... unable to retain the stain, leading to its decolorization. In contrast, counterstain, safranin, is a ...crystal violet dye, but is easily retained in the decolorized gram negative bacteria, staining them red or ...

  17. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type
    Blog Post
    ...wet lab wasn’t the right path for me. I began exploring alternative career trajectories within the industry...self-motivated and excelled when given the freedom to explore projects autonomously. Failing to recognize and...frustration, micromanagement, and disengagement. By tailoring my leadership approach to each person, I was able... a bit to my mentors, as they encouraged me to explore small-scale opportunities where I could make mistakes...role. My advice is: Give yourself permission to explore, reflect on your priorities, and embrace these ...

  18. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type
    Blog Post
    ... Many thanks to our guest blogger David Mellor! David Mellor leads the policy and incentive programs ...This post was contributed by David Mellor from the Center for Open Science. In the last decade, researchers...Before coming to the Center for Open Science, Dr. Mellor worked with citizen scientists to design and implement...at https://orcid.org/0000-0002-3125-5888 and @EvoMellor.  ...

  19. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ... improve the efficiency of editing, including exploring new sources for the reverse transcriptase. Many...

  20. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    ...with spectral flow—you can run a three color panel or a 40 color panel! When preparing a spectral flow ...experiment. For very large panels (upwards of 30-40 colors), spectral flow cytometry might be the better choice... Figure 3: Similarity index across 12 different colors on a three laser spectral flow cytometer. ...from a batch that is not the same as your single color control. Reference controls: Beads or cells? Compensation...
Showing: 61 - 80 of 382 results