Top Requested Lentivirus and AAV of 2016
Type
Blog Post
...pAAV-hSyn-DIO-hM4D(Gi)-mCherry contains a double floxed human muscarinic receptor 4 (Gαi-coupled) DREADD...
We narrowed to 85 results for: Lox
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Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors
TypeBlog Post...popular method uses Cre recombinase on transgenic flox animals to introduce modifications at the DNA level... -
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation
TypeBlog Post...these needs is the lentiviral-based expression of floxed dCas9 so that it may later be excised by Cre recombinase... -
Fluorescent Tagging of Endogenous Genes with SapTrap
TypeBlog Post...nonfluorescent (Halo, SNAP) tags, a selectable marker (floxed Cbr-unc-119) for easy screening of the insertion... -
Hot Plasmids: Summer 2025
TypeBlog Post...endocytosed, the Cre recombinase payload activates a floxed reporter gene (genetically encoded or delivered... -
PCR Applications for Copy Number Variation Assays
TypeBlog Post...N. K., Lee, C. K., Abu Bakar, S., AbuBakar, S., Hollox, E. J., & Boon Peng, H. (2015). A comparison of... -
AAVs CREATed for Gene Delivery to the CNS and PNS
TypeBlog Post...the cap gene. In this special CREATE cap plasmid, loxP sites flank the downstream polyA sequence. When ... -
Deciphering the Mysteries of Behavior with Viral Vectors
TypeBlog Post...-made AAV vectors which can be used in numerous floxed animal species for behavioral tests. Forced ... -
Antibodies 101: The Basics of Western Blotting
TypeBlog Post...(1979) Transfer of proteins from gels to diazobenzyloxymethyl-paper and detection with antisera: a method... -
Mycoplasma Contamination: Where Does It Come From and How to Prevent It
TypeBlog Post...Also, drugs like BM Cyclin, fluoroquinolone ciprofloxacin, ciprobay, zagam, baytril, tetracycline, etc... -
A Primer on Optogenetics: Introduction and Opsin Delivery
TypeBlog Post...some constructs have been engineered with a double-floxed inverse open-reading frame (DIO). Upon coexpression... -
Penn Vector Core Partnership with Addgene
TypeCollection... AV-1-PV2699 100030-AAV1 pAAV.CBA.loxP.ArcLightD.2A.nlsmCherry.loxP.WPRE.SV40 Lawrence Cohen AV-1-PV3509...Svoboda AV-1-20297P 20297-AAV1 pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA Optogenetics Karl...Deisseroth AV-1-20298P 20298-AAV1 pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA Optogenetics Karl ...Deisseroth AV-5-20297P 20297-AAV5 pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA Optogenetics Karl...Deisseroth AV-5-20298P 20298-AAV5 pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA Optogenetics Karl ...Svoboda AV-9-20297P 20297-AAV9 pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA Optogenetics Karl...Deisseroth AV-9-20298P 20298-AAV9 pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA Optogenetics Karl ... -
28 Hot Plasmid Technologies from 2015
TypeBlog Post...terminators and various pieces for inducible expression, loxP sites, H2B, P2A or PEST2D sequences. Several pre-constructed... -
Optogenetics AAV Preps
TypeCollection...dependent 8 Karl Deisseroth 20297 pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA EF1a ChR2/H134R..., 9, rg* Karl Deisseroth 20298 pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA EF1a ChR2/H134R EYFP...Flp dependent 8 Karl Deisseroth 20949 pAAV-double floxed-eNpHR-EYFP-WPRE-pA EF1a eNpHR EYFP Cre dependent... -
Retrograde AAV viral preps
TypeCollection...Viviana Gradinaru 112677 pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP EF1a Color-flipping switch. Expresses...Norepinephrine sensor Yulong Li 20297 pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA EF1a Activator,...Optogenetics Karl Deisseroth 20298 pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA EF1a Activator, Cre-dependent... -
Brain Initiative Collection
TypeCollection... Archon1-KGC-GFP-ER2 under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal...179459-AAV1 pAAV-EF1a-DIO-JEDI-2P-Kv-WPRE Double floxed soma and AIS-localized genetically encoded voltage...St-Pierre 179460-AAV1 pAAV-EF1a-DIO-JEDI-2P-WPRE Double floxed genetically encoded voltage indicator (GEVI) JEDI... -
Retrovirus Plasmids
TypeCollection...puromycin or screen for GFP Bartel 32702 pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE MSCV Conditional overexpression... -
Control AAV Preps
TypeCollection...Constitutive 5 Chun-Li Zhang 20299 pAAV-EF1a-double floxed-mCherry-WPRE-HGHpA EF1a mCherry Cre dependent 9...PHP.S Edward Boyden 112677 pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP EF1a NLS-mCherry or nls-EGFP Cre dependent... -
Zhang Lab CRISPR Page
TypeCollection...2A-EGFP-KASH-WPRE-shortPA-ITR This plasmid enables Cre/loxP recombination and fluorescence assisted sorting ...2A-EGFP-KASH-WPRE-shortPA-ITR This plasmid facilitates Cre/loxP recombination and fluorescence assisted sorting ... -
Genetic Code Expansion
TypeCollection...Mut1-RS tyrosyl-tRNA synthetase M. jannaschii 4-propargyloxy-l-phenylalanine (pPR) Bacterial TAG Miriam Amiram...Mut2-RS tyrosyl-tRNA synthetase M. jannaschii 4-propargyloxy-l-phenylalanine (pPR) Bacterial TAG Miriam Amiram...