We narrowed to 79 results for: Lox

1. Fluorescent Tagging of Endogenous Genes with SapTrap

   Type

   Blog Post

   ...nonfluorescent (Halo, SNAP) tags, a selectable marker (floxed Cbr-unc-119) for easy screening of the insertion...

2. AAVs CREATed for Gene Delivery to the CNS and PNS

   Type

   Blog Post

   ...the cap gene. In this special CREATE cap plasmid, loxP sites flank the downstream polyA sequence. When ...

3. Deciphering the Mysteries of Behavior with Viral Vectors

   Type

   Blog Post

   ...-made AAV vectors which can be used in numerous floxed animal species for behavioral tests.    Forced ...

4. Antibodies 101: The Basics of Western Blotting

   Type

   Blog Post

   ...(1979) Transfer of proteins from gels to diazobenzyloxymethyl-paper and detection with antisera: a method...

5. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

   Type

   Blog Post

   ...Also, drugs like BM Cyclin, fluoroquinolone ciprofloxacin, ciprobay, zagam, baytril, tetracycline, etc...

6. A Primer on Optogenetics: Introduction and Opsin Delivery

   Type

   Blog Post

   ...some constructs have been engineered with a double-floxed inverse open-reading frame (DIO). Upon coexpression...

7. Penn Vector Core Partnership with Addgene

   Type

   Collection

   ... AV-1-PV2699 100030-AAV1 pAAV.CBA.loxP.ArcLightD.2A.nlsmCherry.loxP.WPRE.SV40 Lawrence Cohen AV-1-PV3509...Svoboda AV-1-20297P 20297-AAV1 pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA Optogenetics Karl...Deisseroth AV-1-20298P 20298-AAV1 pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA Optogenetics Karl ...Deisseroth AV-5-20297P 20297-AAV5 pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA Optogenetics Karl...Deisseroth AV-5-20298P 20298-AAV5 pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA Optogenetics Karl ...Svoboda AV-9-20297P 20297-AAV9 pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA Optogenetics Karl...Deisseroth AV-9-20298P 20298-AAV9 pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA Optogenetics Karl ...

8. Optogenetics AAV Preps

   Type

   Collection

   ...Cre dependent 8 Deisseroth 20297 pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA EF1a ChR2/H134R... 5, 8, 9, rg* Deisseroth 20298 pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA EF1a ChR2/H134R EYFP...EYFP Flp dependent 8 Deisseroth 20949 pAAV-double floxed-eNpHR-EYFP-WPRE-pA EF1a eNpHR EYFP Cre dependent...

9. 28 Hot Plasmid Technologies from 2015

   Type

   Blog Post

   ...terminators and various pieces for inducible expression, loxP sites, H2B, P2A or PEST2D sequences. Several pre-constructed...

10. Retrograde AAV viral preps

    Type

    Collection

    ...Control Gradinaru 112677 pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP EF1a Color-flipping switch. Expresses...Norepinephrine sensor Li 20297 pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA EF1a Activator,... Optogenetics Deisseroth 20298 pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA EF1a Activator, Cre-dependent...

11. Brain Initiative Collection

    Type

    Collection

    ... Archon1-KGC-GFP-ER2 under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal...179459-AAV1 pAAV-EF1a-DIO-JEDI-2P-Kv-WPRE Double floxed soma and AIS-localized genetically encoded voltage...St-Pierre 179460-AAV1 pAAV-EF1a-DIO-JEDI-2P-WPRE Double floxed genetically encoded voltage indicator (GEVI) JEDI...

12. Retrovirus Plasmids

    Type

    Collection

    ...puromycin or screen for GFP Bartel 32702 pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE MSCV Conditional overexpression...

13. Zhang Lab CRISPR Page

    Type

    Collection

    ...2A-EGFP-KASH-WPRE-shortPA-ITR This plasmid enables Cre/loxP recombination and fluorescence assisted sorting ...2A-EGFP-KASH-WPRE-shortPA-ITR This plasmid facilitates Cre/loxP recombination and fluorescence assisted sorting ...

14. Genetic Code Expansion

    Type

    Collection

    ...Mut1-RS tyrosyl-tRNA synthetase M. jannaschii 4-propargyloxy-l-phenylalanine (pPR) Bacterial TAG Miriam Amiram...Mut2-RS tyrosyl-tRNA synthetase M. jannaschii 4-propargyloxy-l-phenylalanine (pPR) Bacterial TAG Miriam Amiram...

15. Kazuhiro Oka Lentiviral Vectors

    Type

    Collection

    ...Flp recombinases to remove genes flanked by either loxP or frt sites respectively, and empty vectors to ...

16. Zebrafish Plasmid Collection

    Type

    Collection

    ...Eisenhoffer Lab. An optogenetically controlled Cre/loxP system that enables precise temporal and spatial...

17. Control AAV Preps

    Type

    Collection

    ...PHP.eB, PHP.S Boyden 112677 pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP EF1a NLS-mCherry or nls-EGFP Cre dependent...

18. Caltech Systemic Capsids

    Type

    Collection

    ...Activator ChR2 Deisseroth 20298 pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA EF1a Activator, Cre-dependent...

19. CRISPR History and Development for Genome Engineering

    Type

    Collection

    ...mutations seen in human patients, protein tags, or loxP/FRT sites, among others. Homology-directed repair...