We narrowed to 840 results for: Met;

...between neuroscience and other fields. For instance I met one researcher studying how the microbiome can affect...engineering E. coli metabolism, maybe you’ll be able to apply your knowledge of metabolism to better understand...there were also people ready to discuss their VR helmets, gait tracking technologies, and brain monitors...receptors to the brain, and probing how fatty acid metabolites affect brain function. These scientists pulled...

...TALE fusions with the histone demethylase LSD1 (Lysine specific demethylase) made using these plasmids along...with the FLASH method can decrease the amount of Histone H3 lysine 4 (H3K4) di-methylation at the target...Ekker Golden Gate TALEN 2.0 Golden Gate cloning method. Validated in multiple organisms. Dan Voytas Adam... Zhang Lab TALE Toolbox PCR/Golden Gate cloning method. Optimized for human expression. Feng Zhang LIC...(assembled using the REAL, REAL-Fast, or FLASH methods) for expression as TALE-LSD1 fusions. It was recently...

...phosphoprotein expression in rEcoli XpS in the Methods for iSPI, rEcoli XPS, pSerOTS-C1* (V70) (DOCX, ...phosphoserine in >36,000 phosphosites by tandem mass spectrometry (Barber et al., 2018). This phosphosite library...human substrates of kinases of interest by mass spectrometry in a technique called serine-oriented library...phosphorylated positions for testing phosphoproteomics methods, proteomics search algorithms, proteomics phosphosite...phosphosite localization algorithms, or any method confounded by phosphosite ambiguity. Rinehart Lab Improved...phosphoserine resource for phosphoproteomics . Nature Methods , 19 (11), 1371–1375. https://doi.org/10.1038/s41592...pSerOTS-C1* (V70) (ca. 2022) from the Rinehart lab: Methods for iSPI, rEcoli XPS, pSerOTS-C1* (V70) (DOCX, ...

...Ligation) assembly method (Sander et al., Nat Biotechnol. 2011), the REAL-Fast assembly method (Reyon et al....effectors, including designed TALENs, and assembly methods for REAL, REAL-Fast and FLASH Genome...Automatable Solid-phase High-throughput) assembly method (Reyon and Tsai et al., Nat Biotechnol 2012). All...architectural framework and the DNAs produced by all three methods are identical in sequence. TAL effector repeat ...activators in human cells ( Maeder et al., Nat Methods 2013 ) More detailed information about REAL, REAL-Fast...

...the Materials and Methods of your future publications. For your Materials and Methods section: "The plasmid...Plamids for Oligomerized Pool ENgineering (OPEN) method has been used to engineer ZFNs that function in...ZFNs via the Oligomerized Pool ENgineering (OPEN) method. This kit consists of 5 individually packaged plasmids...2009 Sep 17. PubMed . Description Use of the OPEN method requires OPEN pools (available by request from ...2000 ). The Oligomerized Pool ENgineering (OPEN) method has been used to engineer ZFNs that function in...

...are initially titered by spectrophotometry using a NanoDrop spectrophotometer. If needed, the sample is...Overview of Addgene's antibody production methods and quality control processes for purified recombinant... scientists. They are generated using standard methods individually optimized for each specific antibody...Details about our production protocols, titering methods, and quality control are described below. Information...PCR product is purified, sequenced via the Sanger method, and aligned with the parental plasmid sequence...

...pNCS-CyOFP , mCardinal-N1 mAmetrine* tdTomato 406 0.58 581 138,000 0.69 6.5 7.1 mAmetrine-N1 , tdTomato-N1 mTurquoise2...distance between the fluorophores at the single-nanometer scale, scientists can use FRET to report on protein...Biosensors Collection to find fluorescent reporters for metal ions, small molecules, and more! Popular FRET Pairs...Protocol for deriving proximity, affinity, and stoichiometry of protein interactions using image-based quantitative...its potential while avoiding pitfalls . Nature Methods , 16 (9), 815–829. doi: https://doi.org/10.1038...

...promoter or cloning method of choice. ID Plasmid Description Promoter Cloning Method PI Synthetic Biology...transformation constructs, based on the Golden Gate method. Golden Gate (GreenGate) Jan Lohmann 1000000076...Includes modules for CRISPR/Cas9 genome editing, dexamethasone-inducible expression, and fluorescent plasma...of protein-protein interactions in planta via ratiometric Bimolecular Fluorescence Complementation (rBiFC...

...MK, NEGF2 MET met proto-oncogene (hepatocyte growth factor receptor) AUTS9, HGFR, RCCP2, c-Met MIA melanoma... SDGF ARMET arginine-rich, mutated in early stage tumors ARP, MANF, MGC142148, MGC142150 ARMETL1 arginine-rich...

... and Evolving Functional Proteins. METHODS: A Companion to Methods in Enzymology 8, 94–103 (1995). Carlos...Monoclonals with Engineered Immune Effector Functions. Methods Mol Biol. 2018;1827:313-334. Daniel Christ pComb3...diabody and Fab fragment libraries from chickens Methods for the generation of chicken monoclonal antibody...antibody fragments by phage display. J Immunol Methods. 2000 Aug 28;242(1-2):159-81. Carlos Barbas Vector ...

...with the avid sharing of data, tools, resources, methods, and results that are being adopted in the field...Sclerosis Amyotrophic lateral sclerosis (ALS), sometimes referred to as Lou Gehrig’s disease, is a progressive...TnpBmax , for genome editing. (Marquart et al. Nat Methods. 2024) Base edit human mitochondrial DNA using ... green and red GRAB sensors . (Zhuo et al. Nat Methods. 2023) Browse the Allen Institute's Enhancer Collection..., antibodies, and cell lines. Neurodegeneration Method Development Community (Link opens in a new window...

.... Below are some common delivery methods. Delivery method Advantages Representative Empty ...Inducible gene expression under the metallothionein promoter pFastBac LIC and pFastBac Dual LIC...our dedicated Cre-lox Plasmids page pBF3060 - Metabolic engineering in S. cerevisiae TALENs Gene targeting... Selectable Markers Regardless of your delivery method, it's unlikely that all of your cells will...

...J, Kim SG, Blenis J. Cell Metab. 2014 Mar 4;19(3):373-9. doi: 10.1016/j.cmet.2014.01.001. PubMed PMID:...mTOR pathway. enhanced signaling of mTOR, a key metabolic regulator, correlates with poor prognosis in many...ammalian t arget o f r apamycin (mTOR) is a key metabolic regulator controlling cell growth and proliferation...

...tagging methods allow for improved efficiency and throughput over traditional tagging methods. Several...developed a simple and scalable CRISPR/Cas-based method to tag endogenous proteins by using donor constructs...Seydoux's lab has developed a systematic and scalable method to create marker-free mutations, insertions, and...

...Expression mAmetrine 406 526 26 6 1.8 min Monomer (A206K) pBad-mAmetrine - Bacterial Expression mAmetrine-N1 ...and cloning of fusion proteins, along with key parameters to help you plan your experiment. Tables are ...mAmetrine-N1 - Mammalian Expression mAmetrine-C1 - Mammalian Expression LSSmOrange 437 572 23 5.7 2.3 h Monomer pLSSmOrange-C1...

...payloads in target populations defined by multiple parameters....payloads in target populations defined by multiple parameters. Figure 1: Examples of intersectional cell population...combination of genetic and/or anatomical-defined parameters, by placing two orthogonal recombinase (Cre and...vectors using multiple-feature Boolean logic. Nat Methods 11(7):763-72. PubMed (Link opens in a new window...

...bases without creating a double-strand break. This method displays higher efficiency than HDR-mediated editing...the overall effect is an A->G change. Since this method targets RNA rather than DNA, it does not create...targeting : Cas13 enzymes target RNA rather than DNA, sometimes requiring a protospacer flanking sequence (PFS...biotin-streptavidin pulldown (CAPTURE.) Tag : Multiple methods make it easier to tag endogenous loci with epitope...

...Industry interviews, however, were more structured. I met with several individual and/or small groups, usually...have a theme too, which is something not inherently captured by the STAR method. I like to think of the ...stories to tell in your interview The STAR interview method is a popular way to answer behavioral interview...situation, task, action, and results. While this method seems simple, I found it hard to remember to describe...interview. Each interview is an opportunity to learn something and then apply it to the next interview. Perhaps...underdressed, but read emails from HR carefully because sometimes companies have relaxed dress codes for interviews...

...lysosomes. Though a number of biosensors existed that met some of the criteria necessary for studying endosomes...activate the proteasome. Clift et al. tested multiple methods for TRIM-Away delivery. For small cell populations... recent Nature Communications article “SRpHi ratiometric pH biosensors for super-resolution microscopy...be photostable over a broad pH range. The four biometric probes successfully targeted endosomes under a...modules to repress or activate gene expression, sometimes incorporating chemically inducible approaches ...

...hint of CRISPR editing in human embryos has been met with a storm of media coverage. But the paper published...