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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 94 results for: NIG

Showing: 61 - 80 of 94 results

  4. MXS Chaining

    Type
    Blog Post
    ...875. Pubmed PMID: 13790780. 5. Shetty RP, Endy D, Knight TF. Engineering BioBrick vectors from BioBrick ...

  5. Plasmids 101: Colony PCR

    Type
    Blog Post
    ... liquid cultures. Lastly, you can start small overnight liquid cultures with the clones you pick and only...

  14. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    ...more colonies you will need to pick) and grow overnight cultures for DNA purification. The simplest purification...

  17. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...microcentrifuge Desktop vortexer Vacuum (optional) Reagents Overnight culture of bacteria transformed with your plasmid...Protocol: Generalized DNA Purification Grow an overnight culture of bacteria . Pro-Tip Refer to appropriate... with DNA preparation. Pro-Tip If your entire overnight culture cannot fit into a single centrifuge tube...of dH 2 O Store Solution III at 4°C Grow 2 mL overnight cultures from single colonies of bacteria containing...often thought that an incubation of 20 min to overnight at -20 °C or -80 °C will improve precipitation...mix. Optional: Place the tube either at -20 °C overnight OR -80 °C for 30 min OR on dry ice for 5 min. ... good idea to let it sit for several hours to overnight at room temperature before quantifying and using...

  18. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...protein, Apolipoprotein L3 (red) overnight, blocked, and incubated overnight with Anti-Desmin [D7]. Plates...plate seal and incubate at 37 °C for 30 min , or overnight at 4 °C . Section 2: Block the plate Prepare the...set to 400 rpm for 2 h at room temperature or overnight at 4 °C . Section 3: Primary antibody incubation...set to 400 rpm for 2 h at room temperature or overnight at 4 °C . Section 4: Secondary antibody incubation...set to 400 rpm for 2 h at room temperature or overnight at 4 °C . Section 5: TMB reaction Carefully remove...

  19. Protocol - How to Create a Bacterial Glycerol Stock

    Type
    Protocol
    ...Inoculating an Overnight Liquid Culture . After you have bacterial growth, add 500 μL of the overnight culture...bacteria onto an LB agar plate . Grow your bacteria overnight at the appropriate temperature. Growth conditions...page. The next day you will be able to start an overnight culture for plasmid DNA prep the following day...

  20. CRISPR Library Amplification

    Type
    Protocol
    ...Workflow Timeline Day 1: Transform, recover, set up overnight growth (Estimated time 2-3 hours) Transformation...prewarmed Petri dish. Incubate plates at 30 ℃ overnight. Plate 2.5 mL of the transformed cells on each...certain angle. Incubate plates upside down at 30 ℃ overnight. Critical Ensure at this stage that no unabsorbed...plates remain agar side up until dried before overnight incubation if needed. Place 100 mL sterile LB ...
Showing: 61 - 80 of 94 results