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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 96 results for: NIG

Showing: 61 - 80 of 96 results

  5. MXS Chaining

    Type
    Blog Post
    ...875. Pubmed PMID: 13790780. 5. Shetty RP, Endy D, Knight TF. Engineering BioBrick vectors from BioBrick ...

  6. Plasmids 101: Colony PCR

    Type
    Blog Post
    ... liquid cultures. Lastly, you can start small overnight liquid cultures with the clones you pick and only...

  9. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...disease Petr Svoboda 121165 tau exon 9-12 splicing minigene MAPT CMV Parkinson's, FTD Stefan Stamm 121992 ...Piggybac-TetO-hNurr1-blast NR4A2 TetO Parkinson's Marius Wernig 176852 UBQLN2_450-624W UBQLN2 T7 ALS Carlos Casta...

  16. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    ...more colonies you will need to pick) and grow overnight cultures for DNA purification. The simplest purification...

  19. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...microcentrifuge Desktop vortexer Vacuum (optional) Reagents Overnight culture of bacteria transformed with your plasmid...Protocol: Generalized DNA Purification Grow an overnight culture of bacteria . Pro-Tip Refer to appropriate... with DNA preparation. Pro-Tip If your entire overnight culture cannot fit into a single centrifuge tube...of dH 2 O Store Solution III at 4°C Grow 2 mL overnight cultures from single colonies of bacteria containing...often thought that an incubation of 20 min to overnight at -20 °C or -80 °C will improve precipitation...mix. Optional: Place the tube either at -20 °C overnight OR -80 °C for 30 min OR on dry ice for 5 min. ... good idea to let it sit for several hours to overnight at room temperature before quantifying and using...

  20. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...protein, Apolipoprotein L3 (red) overnight, blocked, and incubated overnight with Anti-Desmin [D7]. Plates...plate seal and incubate at 37 °C for 30 min , or overnight at 4 °C . Section 2: Block the plate Prepare the...set to 400 rpm for 2 h at room temperature or overnight at 4 °C . Section 3: Primary antibody incubation...set to 400 rpm for 2 h at room temperature or overnight at 4 °C . Section 4: Secondary antibody incubation...set to 400 rpm for 2 h at room temperature or overnight at 4 °C . Section 5: TMB reaction Carefully remove...
Showing: 61 - 80 of 96 results