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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 18 results for: NIG

Showing: 1 - 18 of 18 results

  1. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...microcentrifuge Desktop vortexer Vacuum (optional) Reagents Overnight culture of bacteria transformed with your plasmid...Protocol: Generalized DNA Purification Grow an overnight culture of bacteria . Pro-Tip Refer to appropriate... with DNA preparation. Pro-Tip If your entire overnight culture cannot fit into a single centrifuge tube...of dH 2 O Store Solution III at 4°C Grow 2 mL overnight cultures from single colonies of bacteria containing...often thought that an incubation of 20 min to overnight at -20 °C or -80 °C will improve precipitation...mix. Optional: Place the tube either at -20 °C overnight OR -80 °C for 30 min OR on dry ice for 5 min. ... good idea to let it sit for several hours to overnight at room temperature before quantifying and using...

  2. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...protein, Apolipoprotein L3 (red) overnight, blocked, and incubated overnight with Anti-Desmin [D7]. Plates...plate seal and incubate at 37 °C for 30 min , or overnight at 4 °C . Section 2: Block the plate Prepare the...set to 400 rpm for 2 h at room temperature or overnight at 4 °C . Section 3: Primary antibody incubation...set to 400 rpm for 2 h at room temperature or overnight at 4 °C . Section 4: Secondary antibody incubation...set to 400 rpm for 2 h at room temperature or overnight at 4 °C . Section 5: TMB reaction Carefully remove...

  3. Protocol - How to Create a Bacterial Glycerol Stock

    Type
    Protocol
    ...Inoculating an Overnight Liquid Culture . After you have bacterial growth, add 500 μL of the overnight culture...bacteria onto an LB agar plate . Grow your bacteria overnight at the appropriate temperature. Growth conditions...page. The next day you will be able to start an overnight culture for plasmid DNA prep the following day...

  4. CRISPR Library Amplification

    Type
    Protocol
    ...Workflow Timeline Day 1: Transform, recover, set up overnight growth (Estimated time 2-3 hours) Transformation...prewarmed Petri dish. Incubate plates at 30 ℃ overnight. Plate 2.5 mL of the transformed cells on each...certain angle. Incubate plates upside down at 30 ℃ overnight. Critical Ensure at this stage that no unabsorbed...plates remain agar side up until dried before overnight incubation if needed. Place 100 mL sterile LB ...

  5. Protocol - How to Inoculate a Bacterial Culture

    Type
    Protocol
    ... The following protocol is for inoculating an overnight culture of liquid LB with bacteria. Video Watch...You should see no growth in this culture after overnight incubation. Optional: For long term storage of...high or low copy. I didn't get any growth after overnight incubation. What went wrong? Try growing the culture...

  6. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...culture plate. Incubate cells at 37°C, 5% CO 2 overnight. Although cells should regularly be passaged in... in ten 6 cm plates and grow at 37°C, 5% CO 2 overnight. Day 2: b. The target cells should be approximately...Plate target cells and incubate at 37°C, 5% CO 2 overnight. Day 2: b. Target cells should be approximately...cell line. d. Incubate cells at 37°C, 5% CO 2 overnight. Day 3: e. Change to fresh media 24 hours after...

  7. Pouring LB Agar Plates

    Type
    Protocol
    ...them out at room temperature overnight to allow them to dry. After overnight drying, we place the plates...resistant to the antibiotic. Incubate both plates overnight at the appropriate growth temperature and check...

  9. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...the digest goes at least 4 hours and as long as overnight. If you are going to use only one restriction ...more colonies you will need to pick) and grow overnight cultures for DNA purification. After purifying...

  10. Kit Free RNA Extraction

    Type
    Protocol
    ...C for 1 hour, you can try incubating at -80°C overnight. Centrifuge for 20 minutes at 10,000 x g at 4°...

  11. AAV Production in HEK293 Cells

    Type
    Protocol
    ...precipitation. Precipitation of the viruses can proceed overnight at 4 °C if needed. Transfer the entire sample ... prep . The clarified supernatant can be kept overnight at 4 °C before proceeding with the purification...

  13. Fluorescence Titering Assay

    Type
    Protocol
    ...each well of the 6-well dish. Incubate the cells overnight. If using freshly collected virus, filter through...

  17. Western Blot

    Type
    Protocol
    ...typically between 1–10 μg/mL. Incubate the membrane overnight in primary antibody at 4 °C on a rocking platform...
Showing: 1 - 18 of 18 results