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We narrowed to 94 results for: NIG

Showing: 1 - 20 of 94 results

  1. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type
    Blog Post
    ...sequences. Thus, the minigenome contains all cis-acting signals necessary to direct minigenome replication and...Other Important Components of EBOV Minigenome System). However, minigenomes are incapable of producing infectious...alongside a plasmid encoding the minigenome. These genes and the minigenome are transcribed (and the viral...single-stranded RNA minigenome. The viral polymerase complex then recognizes the encapsidated minigenome as a template...Technical Tips). Minigenome replication and transcription In order to replicate the minigenome, the viral polymerase...of the minigenome to produce the positive sense, genome replicative intermediate (anti-minigenome). The...blot analysis or RT-PCR. Uses of the minigenome system Minigenome systems, such as the one described here...

  2. Plasmids for Stem Cell Research

    Type
    Collection
    ...factors. Nature. 2010 Feb 25. 463(7284):1035-41. Wernig Fibroblasts Dopaminergic Neurons Lentiviral Human...Natl Acad Sci U S A. 2012 Feb 14;109(7):2527-32. Wernig Fibroblasts Neural Stem Cells Retroviral Mouse ...conversion. Nat Biotechnol. 2013 May;31(5):434-9. Wernig Fibroblasts Oligodendrocyte Progenitor Cells Lentiviral...

  3. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Erich Nigg 41151 pEGFP Cep170 C-term Centrioles (dependent on cell cycle) Cep170 EGFP Erich Nigg 41163...Centromeres (dependent on cell cycle) PICH EGFP Erich Nigg 158008 pCMV-mGold-Tubulin-C-18 Microtubules alpha-tubulin...

  4. Cre-lox system

    Type
    Collection
    ... pCag FlpO-2A-Cre EV FlpO-2A-Cre CAG Mammalian Breunig 130986 pCag SE (Self-excising) FlpO-2A-Cre EV Self-excising... Self-excising FlpO-2A-Cre CAG Mammalian Breunig 131780 pCS2+PIF6CreN CreLite - Red light-inducible Cre...

  5. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ... can use for long-term library storage. After overnight growth, scrape up, pool and extract plasmid DNA...the plate so you can regrow the lifted colonies overnight), flip the membrane over and place it colony-side...tested, as with any protein purification, but overnight at 30°C is a good start. Prepare a lysis solution...repeated 5 times) works well. Leaving the membrane overnight in the freezer during one of these steps is also...with your cells of interest and growing them up overnight. Assuming they expressed well, the proteins encoded... it can really help to incubate the membranes overnight in 500 mM to 1 M NaCl in order to enhance folding...selective for your target organism and incubate overnight according to appropriate cell growth conditions...

  6. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...a new coordinator for the annual Math & Science Night, I took the reigns. I also volunteered when our ... started coordinating the annual Math & Science Night, a chance for parents and other members of the community...activities. If your school doesn’t have a Math & Science Night (or some equivalent), here are some quick tips to...activities we had for my school’s Math & Science Night. There are plenty of places to get ideas for science... Elementary for trusting me with Math & Science Night, and a special thanks to Joanne Marks, Jonathan ...

  9. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ... virus infection. Microbiology and virology Minigenomes - A Safe Way to Study Dangerous Viruses Contributed...biology while leaving virulent components behind. Minigenomes are stripped down viral genomes containing only... as reporter genes. To learn how you can use minigenomes in your research, check out this post! R Bodies...

  11. Technical Design of a Western Blot

    Type
    Blog Post
    ... your assay. The most common options are 4 °C overnight or 1–2 hours at room temperature (RT). Most people...people choose to incubate their primary antibody overnight at 4 °C and their secondary antibody for 1–2 hrs...membrane. It requires anywhere from sixty minutes to overnight to run, and is recommended for very large proteins...

  12. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    ...displayed!).  The Addgene themed music playing all night was hand-picked (like our plasmid samples!) by Addgenies...Perhaps the most heartfelt portion of the entire night was when Melina and Ken called their parents up ...

  14. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    ... LEGO and science: The potential is endless One night, while opening a LEGO set with my husband, I thought...that resembles a real laboratory. It only took one night to build the first model, but because we are perfectionists...

  17. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    ...or tertiary, or non-existant). On the very first night of the iPSC symposium, a panel of thought leaders...I've already mentioned, Addgene depositors Marius Wernig, Kristin Baldwin, Kathrin Plath, Hans Schöler, ...

  19. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ...Sanger sequencing is done from glycerol stocks (overnight cultures with 15% glycerol), as opposed to purified...handling of large numbers of samples. When we grow overnight cultures for glycerol stock production and storage...

  20. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...daunting. It took a true team effort and many late nights, not unlike most research, but the urgent need ... Sample 6 Cofounder Michael Koeris Learn How Minigenomes Are Being Used to Study Dangerous Viruses Resources...
Showing: 1 - 20 of 94 results