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We narrowed to 562 results for: PAC

Showing: 61 - 80 of 562 results

  1. AAV Viral Preps

    Type
    Collection
    ... (AAV) available from Addgene's viral vectory packaging service. Browse constructs currently available... Viral Vector Packaging Service AAV Browse In-Stock AAV Adeno-associated virus (AAV...can't find the prep you need, try Addgene's AAV Packaged on Request service. You can choose a transfer ...transfer plasmid from our repository and request packaging in one of seven serotypes (AAV1, AAV2, AAV5, AAV6,... For more information, visit our Viral Vector Packaging Service overview. Function Chemogenetics DREADDs...

  4. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ...Browse Lenti Packaged on Request Can't find what you need in our in-stock preps? Our Packaged on Request...the viral vector prep you need. Packaged on Request Viral Vector Packaging Service Information Production... Viral Vector Packaging Service Addgene produces high-quality viral vector...

  5. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    ...Service picks up mail. Recycle plastic packaging Packaging is a huge contributor to the increase in ...lot of their plastic packaging (from Corning®, Falcon®, or Axygen® product packaging) for free! It has to...the lab could make a big difference in our global impact as scientists. Many scientists are already leading...toward responsibility in our lab’s environmental impact, but we can all do more to make research more environmentally...your address sticker off the box, remove the ice pack (it’s not recyclable), tape the box shut, and drop... to be from their company and it has to be packaging (no coated tissue culture plates, for example). Check...and companies have the potential to make a huge impact directly on waste, but also on culture. Encourage...

  6. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...Plasmids Envelope and Packaging Plasmids Popular Lentiviral Envelope and Packaging Plasmids Popular γ-Retrovirus...leukemia virus, and vesicular stomatitis virus G) are packaged as replication-defective virus particles with ...SARS-CoV-2 and other coronaviruses using appropriate packaging plasmids. Use the search bar to find a particular...-Retrovirus Envelope and Packaging Plasmids Reporter Plasmids A few examples of reporter plasmids that...

  7. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...analyses needed for packaging AAV. AAV production Overview: The first step in packaging AAV is transfecting...had never worked with AAV, so naturally I had to package some virus for my first experiment. It was a bit...transfecting HEK293 cells with AAV packaging plasmids. The cells need three different plasmids to produce AAV...intermediate into the single-stranded genome that's then packaged into AAV’s protein shell, or capsid; 2) the pHelper...plasmid which encodes a transgene of interest that’s packaged into the virus. Two to five days after transfection...plasmids contain ITRs which are required for proper packaging of AAV, but also form secondary structures that...spreadsheet similar to this one to plan my AAV packaging PEI transfections. It saves me time and helps ...

  8. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    ...average only 2.8 kb in size and are easy to package in low-capacity vectors! These small, but mighty type VI-D...Cas9! Their small size makes then easy to package in low-capacity vectors like AAV, a popular vector due ...HEPN-independent. These enzymes also do not require a protospacer flanking sequence, so you can target virtually...either an unprocessed or processed gRNA array (22 nt spacer with 30 nt direct repeat) mediating potent knockdown...dementia-associated tau, delivering dCasRx and a 3-spacer array targeting the MAPT exon 10 splice acceptor...toolbox will be. It is, however, certain that the impact of CRISPR scientific sharing will continue to grow...Addgene. Yan, Winston X., et al. “Cas13d Is a Compact RNA-Targeting Type VI CRISPR Effector Positively...

  9. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    ...think we are currently at in the CRISPR software space. The CRISPR research community hasn't had a chance...review process has been challenged with many closely spaced CRISPR software submissions. With few prior publications...GUI”. Trends: Online tools dominate the software space but generally rely on less powerful algorithms to...user in different formats. This can have a large impact on downstream results. Examples: “HTML”, “visual...agnostic tools are the better solution but are often packaged as offline tools requiring expertise in one programming...are important to define for the CRISPR software space. It is unlikely that you will find these terms anywhere... data dump of ALL strings in a genome using any IUPAC encoded pattern at any edit distance. It won’t, ...

  10. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...insert into a viral particle (the packaging signal Psi). Because the packaging plasmids lacked the psi signal...plasmid and the packaging plasmid caused the Psi sequence to be placed on the packaging plasmid forming...virus (MoMLV) and consisted of two plasmids: the packaging plasmid containing all the structural genes necessary...virus. Indeed, by testing several vectors and packaging cell line combinations, Miller et al. showed that...generated by the transfer of a retrovirus into the packaging cell line PA12 (containing the psi deletion). ...viral genes with the exception of the env gene (packaging plasmid), and 3) the vesicular stomatitis virus...293T cells, with your transfer plasmid and your packaging plasmids. For this, you can use the transfection...

  11. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...to using AAV is the limited packaging capacity. Lentiviruses have capacities of 8–12 kb, while AAV only...libraries available at Addgene. The large packaging capacity and stable expression of lentiviral vectors...Lentivirus has limited packaging options, with most lentiviral vectors being packaged using VSV-G. In contrast...contrast, AAV can be packaged in a variety of capsids. To date, at least 12 different capsids, or serotypes...that when using Cas9, you’re already almost out of space! Don’t let this get you down for too long, though...

  12. Plan Your Experiment

    Type
    Collection
    ...vectors is that the packaging limit (how large of a DNA sequence you can actually package into your viral ...Edit References CRISPR ( C lustered R egularly I nterspaced S hort P alindromic R epeats) is a powerful system...efficiencies. The template sequences can also have a large impact on editing efficiency. So while testing multiple...promoters, and selection markers. There are also no packaging limits as there are for viral vectors — the only... Addgene's in-stock AAV preps or try Addgene's Packaged on Request service. Delivery Using mRNA and Proteins...

  14. Western Blot

    Type
    Protocol
    ...gel as follows: Remove the gel from the plastic packaging. Remove tape and plastic comb. Rinse the wells...

  15. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...further! You can package your own lentiviral vector using an integrase-deficient packaging plasmid available... the integrase-deficient packaging plasmid instead of the standard packaging plasmid. For more details...integration of this portion into the host genome. The packaging plasmid contains Gag, Pol, Rev, and Tat genes....transcriptase), and IN (integrase). These viral packaging genes are required for the production of lentiviral...integrated into the host genome via the viral proteins packaged during production (read our blog post on the retroviral...integrated portion does not contain any of the viral packaging genes, the natural cycle of the lentiviral vector...details on how to package lentiviral vectors, check out Addgene’s Lentiviral Guide and our lentiviral production...

  16. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...evolved optimizations for prime editing CHARM: A compact epigenetic silencer Recombinant antibodies for ...assembly of biotinylated SpyTag-SpyCatcher linkers and spacer proteins ending with a GFP nanobody (Figure 1)....determination. A) Schematic of workflow. B) Detail of spacer peptides (3HB: 11-nm 3-helix bundle; SAH: 60-nm...software (green circles), magnetic bead (*), and spacers are labeled; scale bar: 100 nm. D) 3D model of ...other targets. The modular system of linkers and spacers could enable many future variations using nanobodies... the PE components to be delivered in a single package. In a recent publication, the Liu lab showed that...doi.org/10.1038/s41551-024-01227-1   CHARM: A compact epigenetic silencer By Emily P. Bentley The Weissman...

  17. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    ...new teaching space at Lab Central to learn all about it. LabCentral is a lab and office space shared by ...and making space for the techniques ourselves. As you probably have limited time and space in your own...by numerous emerging biotech companies. The space was modern with clean bold colors of orange and white...a few goodies which included an NEB drawstring backpack and a double wall insulated glass to take home...like workshops at Lab Central. Because of the fast pace of work in the Addgene lab, we don’t often get the...opportunity to explore new techniques in our own space. Workshops such as this give us a chance to learn...

  18. Components of CRISPR/Cas9

    Type
    Blog Post
    ...RNA provided that target is upstream (5') of a protospacer adjacent motif (PAM) Cas9 endonuclease binding... RNA and a 3-base pair sequence known as the Protospacer Adjacent Motif or PAM. In order for dsDNA to ... and the unique stretches of DNA between them “spacers” (see figure below). After years of research, we... each direct repeat, combined with its adjacent spacer, ultimately encodes a single crRNA. The direct ...pre-crRNA into mature crRNA and tracrRNA binding. The spacer regions, on the other hand, are the unique, foreign...tracrRNA forms the scaffold portion of a gRNA and the “spacer region” forms the target sequence.   Overview...transcribed into pre-crRNAs containing both the spacer region and the direct repeat region. B) RNase III...

  19. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ... and infect specific cells. This protein shell packages the vector’s genetic material, and variations ...and can be selected as a serotype for Addgene’s Packaged on Request service. Some AAV1 tools are available...and can be selected as a serotype for Addgene’s Packaged on Request service. Some AAV2 tools are available...and can be selected as a serotype for Addgene’s Packaged on Request service. Some AAV5 tools are available...and can be selected as a serotype for Addgene’s Packaged on Request service. Some AAV8 tools are available..., and retinal cells (Issa et al., 2023). AAV9 packaging plasmids are available at Addgene. AAV9 vectors...vectors are not available through our Packaged on Request service, but some AAV9 tools are available as in-stock...

  20. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...ATAACTTCGTATAgcatacatTATACGAAGTTAT 13 bp inverted repeats + 8 bp spacer (34 bp) lox2272, lox5171, lox511, loxN, lox66/lox71...TAACTTTAAATAATgccaATTATTTAAAGTTA 14 bp inverted repeats + 4 bp spacer (32 bp) Currently, only the canonical rox site ...GAAGTTCCTATTCCGAAGTTCCTATTCtctagaaaGTATAGGAACTTC 13 bp inverted repeats + 8 bp spacer (48 bp or 34 bp) F3, F5, FRT14, FRT15 minimal FRT...feature Cre-dependent tools, or consider our AAV Packaged on Request service for more options. Addgene blog...feature Flp-dependent tools, or consider our AAV Packaged on Request service for more options. Addgene blog...
Showing: 61 - 80 of 562 results