Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 68 results for: PCR-amplified REST

Showing: 61 - 68 of 68 results

  1. Molecular Cloning Techniques

    Type
    Guide
    ...is also easy to add restriction sites to any piece of DNA during PCR amplification, allowing for it to...similar to restriction enzyme cloning. To do this, your DNA fragment must first be amplified with specific... rapid cloning of PCR products into donor plasmids without the need for restriction enzyme cloning. Read...the PCR primers for the insert, based on the destination vector sequence and choice of restriction site... Protocols Plasmid cloning by restriction digest Plasmid cloning by PCR DNA ligation Gibson assembly Ligation...molecular cloning, scientists can amplify and manipulate genes of interest and then insert them into plasmids...naturally leaves a single A overhang on the 3' end of PCR products. The complementary T comes from a pre-cut...

  2. Guide to Using Pooled Libraries

    Type
    Guide
    ...surviving cells are collected, their plasmids are PCR-amplified and sequenced using NGS to identify their cDNA...see if it should be amplified before you conduct your screen. If you need to amplify the library, please...Overview of what pooled libraries are; how to amplify and use a pooled library; and what types of screens... 175,000 different gRNA-containing plasmids). Amplifying and Using the Library Once you receive your library...reproducibility. Figure 1: Simplified flow chart for amplifying and using a pooled library. The process diverges...negative screens follow the same basic process: Amplify the library (electroporation and maxiprep). If ... Pooled Library Amplification Protocol for Addgene's generalized library amplification protocol. Read ...

  3. CRISPR References and Information

    Type
    Collection
    ...Sanger sequencing reads from PCR amplicons that cover your locus of interest and correspond to edited and... PDF, 129 KB Marraffini Bacteria: pCRISPR new spacer cloning pCRISPR PDF, 109 KB Mendenhall and Myers ...Download protocol Addgene CRISPR pooled library amplification CRISPR pooled libraries Protocol at Addgene ...CMV-EGFP PDF, 2.4 MB Zhang GeCKO pooled library amplification 1 vector system: lentiCRISPR v2 2 vector system...

  4. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ... along with Cas9 in the T-cell line 2D10. PCR amplification and Sanger sequencing of pooled samples showed...viral protein production, so researchers are also interested in precisely excising the HIV-1 genome from infected...

  5. Molecular Biology Reference

    Type
    Guide
    ... sequence used as an initiation point for PCR amplification or DNA sequencing of the plasmid. Primers ... for the ability of the plasmid to be copied (amplified) by bacteria. Antibiotic Resistance Gene Allows...pieces, labeled with a short DNA barcode, and amplified. These DNA fragments are attached to a glass slide...separated. These attached DNA templates are then amplified again, producing ~1,000 copies of each template...plasmid using bacteria, which take up plasmids and amplify them during cell division. Because bacteria are...Additionally, the restriction enzyme site(s) allow for the cloning of a DNA fragment of interest into the plasmid...contains several restriction enzyme sites, enabling easy insertion of DNA by restriction enzyme digestion...

  6. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...Ultracentrifugation AAV Titration by ddPCR AAV Titration by qPCR Using SYBR Green Technology Content last...containing the transgene or genetic elements of interest to introduce in cells. A packaging plasmid (or...expressing Rep2 and PHP.eB Cap controlled by tTA-TRE amplification system Viviana Gradinaru 103006 pUCmini-iCAP-PHP.S...expressing Rep2 and PHP.S Cap controlled by tTA-TRE amplification system Viviana Gradinaru 127847 pUCmini-iCAP-PHP.V1...expressing Rep2 and PHP.V1 Cap controlled by tTA-TRE amplification system Viviana Gradinaru 175004 pUCmini-iCAP-AAV.CAP-B10...expressing Rep2 and B10 Cap controlled by tTA-TRE amplification system Viviana Gradinaru 175005 pUCmini-iCAP-AAV.CAP-B22...expressing Rep2 and B22 Cap controlled by tTA-TRE amplification system Viviana Gradinaru 185136 pUCmini-iCAP-AAV.MaCPNS1...

  7. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...activation kinetics and high responsivity near the resting membrane potential, ASAP5 is a great new option... an enhanced pU6cm promoter, viral replicon amplification of cargo, and heat treatment.  Figure...CLAP) with a flexible linker where a protein of interest can be inserted. B) Protein expression and labeling...studies of kinesins but for many other proteins of interest too! Find CLIP, CLAP, and SNAP plasmids here! ...biosensors kit By Mike Lacy G protein-coupled receptors (GPCRs) are critical components of many physiological pathways...targets. To enable large-scale profiling of many GPCRs, Mikel Garcia-Marcos’ lab recently developed a collection... detection of activation triggered by endogenous GPCR. Example applications include large-scale pharmacological...

  8. CRISPR Guide

    Type
    Guide
    ...enzymes to bind target DNA PCR P olymerase C hain R eaction; Used to amplify a specific sequence of DNA...experiments and must be amplified before use (Figure 8C), following the amplification protocol specified by...conditions: The sequence is unique compared to the rest of the genome The target is present immediately ...verify that the composition of your amplified library matches that of the original library. Next, you’... copies of the aptamer improves stability and amplifies the signal for better imaging of genomic loci,...Publications CRISPR Overview Bacteria have an interesting adaptive immune response that detects and eliminates... Cas enzyme and a gRNA specific to the gene of interest. The genomic target of the gRNA can be any ~20...
Showing: 61 - 68 of 68 results