We narrowed to 207 results for: PHI-1

...degradation or electrophile signaling. Many of these tools can be found in Addgene's repository (Table 1). ...the best performance in the most contexts (Figure 1). Together the features with optimal biological performance...described as Click Biology (Howarth, 2025). Figure 1: Modular assembly tools, alongside criteria for Click...coupling. Created with BioRender.com.   Table 1: A handful of Click Biology tools available at Addgene... intein; N-terminal CLm and C-terminal Aes gp41-1 Split inteins A small, fast split intein SpyTag003...

... handed out at the event). Program 0 - 1:24 - Intro 1:24 - 29:31 - Reproducibility Overview - Jeffrey... 49:45 0 1:18:45 - Reagent Development - Steven C. Almo, Institute for Protein Innovation  1:18:45 - 2...reproducibility As Jeffrey Flier points out in his talk (1:24 - 29:31), there are a variety of ways of defining...enterprise towards greater reproducibility. Cause 1: Inability to repeat previously performed experiments...the Institute for Protein Innovation (IPI) (49:45 - 1:18:45), there are also initiatives to create more ...found. Consider the hypothetical situation in Figure 1 where many scientists are performing similar experiments...Editor at Science) Edward J. Hall (Professor of Philosophy at Harvard University) Tony Cijsouw (Neuroscience...

... Eric Campeau 21474 pLenti CMV V5-LUC Blast (w567-1) Firefly CMV Lentiviral expression of firefly luciferase...luciferase and gene of interest Scott Lowe 21375 pHIV-Luciferase Firefly Lentiviral vector for insertion...stability and light output Sanjiv Sam Gambhir 104587 pHIV-iRFP720-E2A-Luc Firefly EF1α Lentiviral expression...

...phosphorylated DNA termini to 3 ́-OH DNA termini (1-4). In vitro, ligases (notably T4 DNA ligase) are ... enzymology of DNA repair enzymes.     References 1. Shuman, Stewart, and Christopher D. Lima. "The polynucleotide...thermostable DNA ligase-encoding gene." Gene 109.1 (1991): 1-11. PubMed PMID: 1756968. 7. Lauer, G., et al. "Cloning...and even include profiling single nucleotide polymorphisms (SNPs) (5,12,13). For example, in the Ligase...sequence, and engineered expression of Thermus thermophilus DNA ligase, a homolog of Escherichia coli DNA...Identification of essential residues in Thermus thermophilus DNA ligase." Nucleic acids research 24.15 (1996...for characterization of resistance-mediating polymorphisms in African samples of Plasmodium falciparum....

... fusions that retain their individual activities (1). Researchers also discovered the modular format of...systems could be combined effectively (3). In Figure 1 for instance, the DBD of the fusion protein shown ...ubiquitin system has been devised (8). References 1. Casadaban M.J., Martinez-Arias A., Shapira S.K., ...now many variations, including many that use auxotrophic rescue genes as reporters and some that repress...example of the former. In this system, histidine-auxotrophic host strains are used to select for binding-dependent...

...is expanding rapidly.  References 1. Kunjapur, Aditya M., Philipp Pfingstag, and Neil C. Thompson. "Gene...from distantly related organisms. In 2006, less than 1% of the genes deposited at Addgene were synthetic.... student at the time, got involved. Along with Philipp Pfingstag, a visiting graduate student from TU ...

...NgAgo takes a different approach, randomly removing 1-20 nucleotides from the cleavage site specified by...About NgAgo User's First Impressions Here References 1. Gao, Feng, Xiao Z. Shen, Feng Jiang, Yongqiang Wu... et al. characterized Argonaute from Thermus thermophilus (TtAgo), showing that this protein could cleave...orthologs similar to TtAgo and PfAgo, another thermophilic Argonaute. They identified NgAgo and showed ...

...harness non-homologous end joining repair (NHEJ) [1]. Our initial work was with TALENs, in collaboration...from the University of Pennsylvania.     References: 1. Lee HJ, Kim E, Kim JS. Targeted chromosomal deletions...using zinc finger nucleases. Genome Res. 2010 Jan;20(1):81-89. PubMed. 2. Bauer DE, Kamran SC, Lessard S,...these may require restriction fragment length polymorphism analysis, allele-specific PCR, T7EN1 cleavage...

...guide for a refresher on CRISPR/Cas9.  Figure 1: Cpf1 is a single RNA-guided endonuclease of a class... than the multi-subunit complex employed by class 1 systems. A putative new class 2 nuclease, Cpf1 (CRISPR...that Cpf1 can tolerate mismatches at gRNA positions 1, 8, 9, and 19-23. Accordingly, the 3’ end of the gRNA...Most gRNAs directed low-frequency Cpf1 cleavage at 1-12 off-target sites; in contrast, SpCas9 may cleave...the diversity of CRISPR-based systems. References 1. Zetsche, Bernd, et al. "Cpf1 is a single RNA-guided... off-target editing rates, determined using sophisticated genome-wide analysis. At many of its computationally...

...follow her on Twitter @nieminm.   References 1. Cohen, Philip. "The origins of protein phosphorylation."...cellular proteins are modified through phosphorylation (1). However, the expansion of studies on protein phosphorylation...three components required for translation (Figure 1): A tRNA that recognizes a specific ‘open’ codon ... open reading frame (ORF) of interest (Figure 2). 1. The phosphoserine OTS The backbone of the phosphoserine...modification allowed the deletion of release factor 1, the protein responsible for terminating translation...Rinehart lab: pCRT7 NT TOPO E17TAG GFP and pGEX-6P-1. The pCRT7 NT TOPO vector contains a mutant pSer-containing...

... compounds with various antimicrobial activities (1). She has developed a fast endophyte screening system...us coordinate and promote the awards. References 1. Martinez-Klimova, Elena et al. “Endophytes as sources...antibiotic” Biochemical Pharmacology 2017 Jun 15;134:1-17. PubMed PMID: 27984002. 2. Bilan, Dmitry S. et ...working in a wide range of research fields and geographical locations. We are excited to see how all of ...

...depths (1 mm), important for certain applications such as in vivo tissue imaging.   Figure 1: Ace-mScarlet...sources – voltage-gated potassium channels found in Drosophila to phosphatases from sea squirts. This design... detector in this system was dipicrylamine, a lipophilic compound that distributes itself within the plasma...sensing) hydrolyzes a precursor dye to make it more lipophilic, increasing its affinity for the membrane, where... fluorescent component (i.e., when the dye is lipophilic and integrates into the membrane), the voltage...

...acceptor fluorophore. The typical steps in FRET are: 1. Donor fluorophore excitation by absorption of a photon... overlap, the fluorophores must be located within 1-10nm of each other and be oriented appropriately for... from various papers to make the decision easier. 1. Müller, Sara M., et al. "Quantification of Förster...diseases and even assist in drug discovery. References 1. Baird, Geoffrey S., et al. "Circular permutation ...have enhanced the ability of labs to answer sophisticated questions about cellular processes. Currently...

...following four components into 293T cells. Figure 1: The key components of Nanoblades. Image from Mangeot...protein. A single Nanoblade particle can be loaded with 1-4 different gRNAs. Viral envelope proteins To alter... between ~50-70% for these four cell types (Table 1). Additionally, hiPSCs and mouse BM cells still behaved...hepatocytes  Myd88  ~50% Human HSCs  Myd88  ~50% Table 1: Nanoblades editing efficiencies of primary cells....accessible genome editing tool.   References Mangeot, Philippe E., et al. "Genome editing in primary cells and...

... hybridization experiments. A recent publication [1] from the Sánchez lab describes the plasmids and protocols...deposit) are described in the Sánchez lab publication [1] so that the students performing these experiments...can easily investigate in planarians. References 1. Alice Accorsi, Monique M. Williams, Eric J. Ross,...Proceedings of the National Academy of Sciences 100.suppl 1 (2003): 11861-11865. PubMed PMID: 12917490. PubMed...pp. 806-811. PubMed PMID: 9486653. 3. Newmark, Phillip A., et al. "Ingestion of bacterially expressed ...

... the example shown in Figure 1, large sequences corresponding to exons 1 and 3 of the targeted gene have...knock out exon 2 of a given gene.   Figure 1: A knockout targeting vector designed to insert a ... the Neomycin resistance (NeoR) gene between exon 1 and 3 of the targeted gene. Upon recombination, the...can be used to replace genes in yeast, mice, or Drosophila through homologous recombination. The concept...

... @Addgene congrats for 1 million orders. @RothLabUNC accounts for 10,000 or 1%! — BryanRoth (@zenbrainest... scientific sharing community (check out the infographic below for stats on our plasmid deposits and requests...

...on-target and off-target editing efficiency Figure 1: Effects of (A) double and (B) single base pair gRNA-target...that Cpf1 can tolerate mismatches at gRNA positions 1, 8, 9, and 19-23. Accordingly, the 3’ end of the gRNA...Most gRNAs directed low-frequency Cpf1 cleavage at 1-12 off-target sites; in contrast, SpCas9 may cleave...fits into the genome editing landscape. References 1. Kim D, Kim J, Hur JK, Been KW, Yoon SH, Kim JS. Genome-wide... off-target editing rates, determined using sophisticated genome-wide analysis. At many of its computationally...

...attract fish for them to prey upon (Figure 1). Figure 1: The sea anemone's red color can be used ...synthetic biology. Journal of Biological Engineering, 12(1). https://doi.org/10.1186/s13036-018-0100-0 Murakoshi...expression in living cells. Scientific Reports, 9(1). https://doi.org/10.1038/s41598-019-48604-4 Salih...surface of the chromoprotein Ultramarine with hydrophilic amino acids to reduce non-specific interactions...

...oxygen. Additionally, its status as a Biosafety Level 1 microbe makes it a perfect candidate for introductory...Transport, biofilms, and much more. Because of it’s BSL-1 status, E. coli can be used as a model to relate these...with superior performance. Nature Communications, 9(1). https://doi.org/10.1038/s41467-018-04899-x Yokoyama..., or frogs, are one of the most popular model amphibians in science with early research providing insight... Jiang, J. P. (2016). Research proceedings on amphibian model organisms. Dong wu xue yan jiu = Zoological...