We narrowed to 402 results for: SUA

...membrane, stained with antibodies, and finally visualized.   This process allows you to detect a single... the proteins are immobilized onto a membrane, usually composed of a nitrocellulose or polyvinylidene ... Stuart Orkin's and Daniel Bauer's labs.    Visualizing your protein of interest with the help of secondary...secondary antibodies In order to visualize proteins on the membrane the antibodies are typically conjugated...species-specific secondary antibody is used to visualize the proteins. In this set-up, multiple secondary...

... cover some screening methods to identify them. Visual screening Regardless of the RNAi-trigger type you...As a plant researcher, the traditional hpRNA and visual screening method was limited in two substantial...labor-intensive processes to isolate RNAi plants. With a visual reporter of RNAi, phenotypes, such as morphological...specific target required physical isolation of the visually identified subpopulation. For traditional cell... analysis. Instead, microscopic plants must be visually identified as undergoing RNAi through the fluorescent...

...protein is targeted with an antibody, you can visualize the protein via fluorescence or chemiluminescence...and tissue labeling Antibodies can be used to visualize tissues (immunohistochemistry) or cells (immunocytochemistry...preserved, this technique allows you to not only visualize the presence of specific proteins, but their localization...contextualize its localization. These could be visualized using fluorophore-conjugated antibodies (i.e.... using an unconjugated primary antibody, then visualized using a fluorophore-conjugated secondary antibody...

...for direct visualization while epitope tags are used for both direct and indirect visualization. Some of...alternative to amylose resin and also provides a visual detection mechanism via applications such as western...affinity tags can sometimes be repurposed for visualization. However, due to the nature of the size, structure...

... has been incorporated into the host organism, usually achieved with the proper selection marker or antibiotic... fluorescent fusion protein will allow you to visualize YGOI in vivo (try pcDNA3-EGFP). If you can get...various markers and promoters. If you need to visualize YGOI on a western blot or under a microscope, ... Pick your promoter, selection cassette and visualization vehicle to conduct your custom experiment. Fly...

...The first read At the first read of the paper, I usually try to grasp the main message of the work. I underscore...the given context. After this first read, I am usually able to construct the beginning of my review - ...another day - preferably the following morning - as usually deadlines are pressing. During this second read... (3) development of tools for data analysis/visualization using R/Shiny. She is passionate about open ...

... then its concentration is detected through visualization of an enzymatic reaction. Types of ELISA There...its substrate is added and the reaction can be visualized via a colorimetric reaction. The direct ELISA...secondary antibody is conjugated to an enzyme for visualization. The indirect ELISA is used for similar experiments...additional amplification step, which helps to visualize low-abundance proteins. Figure 4: Overview...

...protein tag for visualization (Fig. 1b). While the probes target chromatin to visualize DNA, an editor ...fluorescent protein can still be included for visualization purposes, but the key component of the editing...result in depletion of the FP, which can be easily visualized.  The tool-kit consists of highly useful vectors...

...Note: If you intend to do a miniprep, you will usually want to start 2 mL in a Falcon tube. For larger...single bacterial cell (Figure 2). Large plasmids usually have a low copy number (approximately one or two...

...The ocean is full of striking visuals from fluorescent jellyfish, bioluminescent phytoplankton, and colorful...shorter history. In 1987, scientists described an unusual blue-colored protein from the jellyfish Cassiopea...methods like sequencing are more definitive, but visual identification is a good place to start before ...FRET References Blanquet RS, Phelan MA (1987) An unusual blue mesogleal protein from the mangrove jellyfish...

...sticky ends that were generated by EcoRI digestion: Usually, scientists select two different enzymes for adding...Ratio Although a 3:1 insert to vector ratio is usually sufficient, you can optimize the amount of insert...

...replication, selection, and expression. Replication It’s usually not enough to get your vector into your target ...both target species. Fun fact! Plant vectors are usually designed to shuttle between E. coli and Agrobacterium...a reporter, such as a fluorescent protein, to visualize vector uptake. These reporters might be used as... as markers for automated cell sorting or to visually select cells in a microscopy image for individual...

...proteins like mScarlet are essential tools for visualizing biological processes in vivo. However, mScarlet...will be a useful addition to the toolkit for visualizing cellular processes in C. elegans: mScarlet3 for...Figure 5). The researchers used the reporter to visualize biologically relevant forces at focal adhesions...colocalizes with the focal adhesion marker paxillin (visualized with GFP). Reprinted with permission from Zhong...

...Researchers have used oligonucleotides in fluorescent visualization techniques for decades, most notably for fluorescent...where fluorophore-linked antibodies are used to visualize RNA. Low signal-to-noise ratios with such techniques...such background, thereby improving traditional visualization techniques. In addition, their incredible versatility...malachite green (MG) to create a non-protein based visualization technique. Ultimately, MG proved to be too toxic...

...and is therefore inefficient in mammalian cells (usually grown at 37 °C). Smart molecular evolution led ...Logie and Stewart 1995; Metzger et al. 1995). Visualizing single neurons in the living brain   ...structure of a neuron and its synapse should be visualized at the same time from two different plasmids ...together with inducible Cre recombinase (CreERT2), visualizes individual neurons and their synapses in a conditional...

...recognition and “productivity”, so this is unlikely. Usually, the originating scientist and colleagues are privileged... amounts of data. When published, this data is usually condensed into a statistically evaluated set of...standards of data recording are highly variable and usually left to individual researchers. Yet accurate and...publications presenting a polished, standard and usually limited view of data. In addition to enhancing ...

...(ii) no need to add a co-factor, (iii) easy visualization with standard filter sets on a fluorescence ...lipids. Still, these molecules can be indirectly visualized by using protein domains that specifically bind...the sample. Although bioluminescent probes are usually dim relative to fluorescent probes, recent engineering...Adjobo-Hermans, Merel JW, et al. "Real-time visualization of heterotrimeric G protein Gq activation in...

...the fine points of visual aids. Here’s a great slideshare with more details on visuals in science presentations...talk (not a lab meeting) in the previous year. Usually fewer than 10% have done so. How can you expect...Finally, don’t pass up opportunities to show cool visuals, but don’t overdo the effects. It may be a bit ...

...sequence on either side of the desired mutation (usually 1-3 mismatched bases) is sufficient for your primers...sequences, but if present, a minor extension can usually ensure that the 3’-base(s) do not form secondary...facilitated by the addition of DMSO to the PCR reaction (usually around 3% final concentration). The DMSO reduces...plasmid. Note that any residual parental plasmid (usually from incomplete DpnI digest) can also form colonies...

... student at Moffitt Cancer Center. Engineering usually calls to mind building things, lots of math, and...sequences and structures to affect their function. Usually, the goal is to enhance their efficacy, safety,...complete each cycle, your best-performing mutant is usually the starting point for the next cycle. ...of different primers. While error-prone PCR is usually employed to mutate whole antibody sequences, degenerate...