Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 420 results for: SUA

Showing: 61 - 80 of 420 results

  1. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty...

  3. Protocol - How to Inoculate a Bacterial Culture

    Type
    Protocol
    ...Note: If you intend to do a mini-prep you will usually want to start 2 mL in a falcon tube, but for larger...within a single bacterial cell. Large plasmids usually have a low copy number (approximately one or two...

  5. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...Researchers have used oligonucleotides in fluorescent visualization techniques for decades, most notably for fluorescent...where fluorophore-linked antibodies are used to visualize RNA. Low signal-to-noise ratios with such techniques...such background, thereby improving traditional visualization techniques. In addition, their incredible versatility...malachite green (MG) to create a non-protein based visualization technique. Ultimately, MG proved to be too toxic...

  6. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ...replication, selection, and expression. Replication It’s usually not enough to get your vector into your target ...both target species. Fun fact! Plant vectors are usually designed to shuttle between E. coli and Agrobacterium...a reporter, such as a fluorescent protein, to visualize vector uptake. These reporters might be used as... as markers for automated cell sorting or to visually select cells in a microscopy image for individual...

  7. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...proteins like mScarlet are essential tools for visualizing biological processes in vivo. However, mScarlet...will be a useful addition to the toolkit for visualizing cellular processes in C. elegans: mScarlet3 for...Figure 5). The researchers used the reporter to visualize biologically relevant forces at focal adhesions...colocalizes with the focal adhesion marker paxillin (visualized with GFP). Reprinted with permission from Zhong...

  8. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ...and is therefore inefficient in mammalian cells (usually grown at 37 °C). Smart molecular evolution led ...Logie and Stewart 1995; Metzger et al. 1995). Visualizing single neurons in the living brain   ...structure of a neuron and its synapse should be visualized at the same time from two different plasmids ...together with inducible Cre recombinase (CreERT2), visualizes individual neurons and their synapses in a conditional...

  9. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    ...recognition and “productivity”, so this is unlikely. Usually, the originating scientist and colleagues are privileged... amounts of data. When published, this data is usually condensed into a statistically evaluated set of...standards of data recording are highly variable and usually left to individual researchers. Yet accurate and...publications presenting a polished, standard and usually limited view of data. In addition to enhancing ...

  10. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty...

  12. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty...

  14. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty...

  15. CRISPR Plasmids - Parasites

    Type
    Collection
    ...Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty...

  17. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...(ii) no need to add a co-factor, (iii) easy visualization with standard filter sets on a fluorescence ...lipids. Still, these molecules can be indirectly visualized by using protein domains that specifically bind...the sample. Although bioluminescent probes are usually dim relative to fluorescent probes, recent engineering...Adjobo-Hermans, Merel JW, et al. "Real-time visualization of heterotrimeric G protein Gq activation in...

  18. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ...the fine points of visual aids. Here’s a great slideshare with more details on visuals in science presentations...talk (not a lab meeting) in the previous year. Usually fewer than 10% have done so. How can you expect...Finally, don’t pass up opportunities to show cool visuals, but don’t overdo the effects. It may be a bit ...

  19. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...sequence on either side of the desired mutation (usually 1-3 mismatched bases) is sufficient for your primers...sequences, but if present, a minor extension can usually ensure that the 3’-base(s) do not form secondary...facilitated by the addition of DMSO to the PCR reaction (usually around 3% final concentration). The DMSO reduces...plasmid. Note that any residual parental plasmid (usually from incomplete DpnI digest) can also form colonies...

  20. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty...
Showing: 61 - 80 of 420 results