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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 424 results for: SUA

Showing: 61 - 80 of 424 results

  1. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...proteins like mScarlet are essential tools for visualizing biological processes in vivo. However, mScarlet...will be a useful addition to the toolkit for visualizing cellular processes in C. elegans: mScarlet3 for...Figure 5). The researchers used the reporter to visualize biologically relevant forces at focal adhesions...colocalizes with the focal adhesion marker paxillin (visualized with GFP). Reprinted with permission from Zhong...

  2. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ...replication, selection, and expression. Replication It’s usually not enough to get your vector into your target ...both target species. Fun fact! Plant vectors are usually designed to shuttle between E. coli and Agrobacterium...a reporter, such as a fluorescent protein, to visualize vector uptake. These reporters might be used as... as markers for automated cell sorting or to visually select cells in a microscopy image for individual...

  3. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    ...recognition and “productivity”, so this is unlikely. Usually, the originating scientist and colleagues are privileged... amounts of data. When published, this data is usually condensed into a statistically evaluated set of...standards of data recording are highly variable and usually left to individual researchers. Yet accurate and...publications presenting a polished, standard and usually limited view of data. In addition to enhancing ...

  4. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...(ii) no need to add a co-factor, (iii) easy visualization with standard filter sets on a fluorescence ...lipids. Still, these molecules can be indirectly visualized by using protein domains that specifically bind...the sample. Although bioluminescent probes are usually dim relative to fluorescent probes, recent engineering...Adjobo-Hermans, Merel JW, et al. "Real-time visualization of heterotrimeric G protein Gq activation in...

  5. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ...the fine points of visual aids. Here’s a great slideshare with more details on visuals in science presentations...talk (not a lab meeting) in the previous year. Usually fewer than 10% have done so. How can you expect...Finally, don’t pass up opportunities to show cool visuals, but don’t overdo the effects. It may be a bit ...

  6. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...sequence on either side of the desired mutation (usually 1-3 mismatched bases) is sufficient for your primers...sequences, but if present, a minor extension can usually ensure that the 3’-base(s) do not form secondary...facilitated by the addition of DMSO to the PCR reaction (usually around 3% final concentration). The DMSO reduces...plasmid. Note that any residual parental plasmid (usually from incomplete DpnI digest) can also form colonies...

  7. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    ... student at Moffitt Cancer Center. Engineering usually calls to mind building things, lots of math, and...sequences and structures to affect their function. Usually, the goal is to enhance their efficacy, safety,...complete each cycle, your best-performing mutant is usually the starting point for the next cycle. ...of different primers. While error-prone PCR is usually employed to mutate whole antibody sequences, degenerate...

  8. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty...

  9. Pouring LB Agar Plates

    Type
    Protocol
    ... bacteria with resistance to that antibiotic - usually conferred by a plasmid carrying the antibiotic ...H 2 O Sterile plates of your desired size - we usually use 60 mm x 15 mm plates which can hold 5-10 mL...

  10. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ...Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty...

  12. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty...

  15. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty...

  17. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty...

  18. 6 Tips for Analyzing and Troubleshooting Sanger Sequencing Results

    Type
    Blog Post
    ...proper ladder. These controls help you properly visualize your results.The most important of those is to...comes to DNA sequencing the chromatogram is your visual control. And, like all controls, missing out is...bases of a DNA sequencing read The peaks here are usually unresolved and small, so I suggest designing your...

  20. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty...
Showing: 61 - 80 of 424 results