Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 456 results for: SUP

Showing: 61 - 80 of 456 results

  1. Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

    Type
    Blog Post
    ...scientists. Many members of the scientific community support open sharing. But, one critical part was missing...universities are showing an unprecedented amount of support about allowing use of plasmids for research by ...weeks. As of June 2020, we are happy to have the support of the following organizations to share materials... synthesized approximately 3,000 constructs to support SARS-CoV-2 research.  Browse the entire Ginkgo ...and funding initiatives such as Fast Grants that support us in our mission.  While rapidly expanding our...

  2. Scientific Sharing in the Time of COVID-19: Databases and Resources

    Type
    Blog Post
    ... world. To support this pace of research, new, centralized databases and centers to support the sharing...like here at Addgene!), and new databases are supporting their access to useful resources during this ...research Universities have also been stepping up to support COVID-19 research. The new Center for COVID-19 ...Preparedness (CCRP2) at Rutgers University not only supports collaborations at Rutgers, but also lists data...

  3. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

    Type
    Blog Post
    ... Accelerating science and supporting scientists globally are the shared mission of both Seeding Labs ...well as developing yeast as source for dietary supplements! Development of a bacterial expression system...plants.     Transgenic yeast as source of dietary supplements Dr. Mrinalini Nair, University of Baroda, India...proteins in order to manage insect pests and to support sustainable agriculture. For this project the expression...in Mexico. At Addgene we're happy to be able to support diverse groups of scientists working in a wide ...

  4. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    ...lifetime supply for about $10 US dollars worth of glass powder pruchased from a pottery supply house.  ...either.  Test tubes aren’t cheap everywhere Lab supplies and equipment are more precious in less resourced...Labs does an amazing job shuttling unwanted lab supplies to places that really need them.  When I was in...stop me. I only had to replenish my glass milk supplies every 10 years or so. Actually, I was happy to...

  5. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    ...and have always been, a community resource that supports both toolmakers and tool users. We encourage you...Educational Resources As part of our commitment to supporting your research, Addgene is creating an array of...Through our incredible community, including your support, we have enabled scientists to easily share plasmids...Studies Hybridoma Bank, a nonprofit that has been supporting hybridoma sharing within the community for decades...Immunofluorescence Addgene’s Antibody Guide The project is supported by a NIH BRAIN Initiative grant (U24NS119916 )...

  6. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...PA-FPs have helped to revolutionize the field of super resolution microscopy. PA-FPs, especially the monomeric...monomeric, reversible type, are very well suited for super resolution imaging, where the power of the technique... reconstruct an image. The best PA-FPs used for super-resolution imaging usually have the following characteristics-high...fluorescence wavelengths. Indeed, within the world of super resolution microscopy, PA-FPs have helped create...are vastly improved over the conventional FPs for super-resolution imaging, they still do not emit the large...being engineered. By combining PA-FPS with superresolution microscopy, scientists now have at their fingertips...

  7. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    ...classes and participating in these is a great way to support your favorite local exercise spot or find something...PhysicalDistancing. Social interactions and #social support are even more necessary during these times of stress... stress due to #COVIDー19. Receive and provide support while being safe. @GHP_HarvardChan — Shekhar Saxena...testing for COVID-19. You can also give back by supporting local businesses (ex: purchasing a gift card .... Think about reproducibility best practices Support your future experiments and other researchers by...

  8. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    ... inducing immunosuppressive measures, for example the upregulation of the immunosuppressive cell surface...factors for chronic inflammation which in turn suppresses cancer immunosurveillance. The presented study...surface receptor PD-1. This immunosuppressive environment in turn may represent a favorable state for tumor...Future treatments may need to target this immunosuppressive reaction in order to improve therapeutic outcomes...

  9. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    ...You need the support of your institute to do this one. Universities will usually only support tenure track...This criterion is best proven through letters of support from experts in the field. You must also be able...Performing peer review at the request of your supervisor is not considered peer review. The peer review...very attractive option.    Your Expert Letters of support can mean that your case gets approved, denied or...Request for Further Evidence (RFE). Letters of support are the only opportunity that you are given to ...

  11. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    ...particular the roles of proto-oncogenes, tumor suppressors, and their protein products. Proto-oncogenes ...survival, growth and proliferation, while tumor suppressors perform the opposite task, keeping cells from...compare the roles of proto-oncogenes and tumor suppressors to the accelerator and brake pedal of a vehicle...down. Similarly, a mutation that turns a tumor suppressor “off” is analogous to a brake pedal that does...specific genes involved. Downregulation of tumor suppressors and upregulation of proto-oncogenes ­­­can directly...

  12. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...your media with supplements such as magnesium salts, buffering agents, and/or supplying additional carbon... in copy number, the number of plasmid copies supported within each bacterial cell in your culture. Copy...culture media Changing to a richer media or adding supplements can improve your cell density, leading to an ...normally recommended antibiotic concentration. Add supplements to the media You can achieve higher cell density...

  13. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...features previously only visible via techniques like super-resolution microscopy or electron microscopy. Since... of specific targets you can label in a sample. Super-resolution microscopy (SRM), a family of imaging...targets. What are some of the limitations?  A “super-resolution” technique that uses common reagents,....73775  Dani A, Huang B, Bergan J, et al (2010) Superresolution Imaging of Chemical Synapses in the Brain. ...Diederich B, Heintzmann R, Schermelleh L (2022) Super-resolution microscopy: a brief history and new avenues...

  14. Viral Production

    Type
    Collection
    ...Approximately 2 weeks post-thaw, cell culture supernatant is tested for mycoplasma contamination. To date...

  15. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ...efficiency supE44 (glnV44)   Suppression of the amber (UAG) stop codon by glutamine insertion supF (tyrT)...tyrT)   Suppression of the amber (UAG) stop codon by tyrosine insertion λ-thi-1 or thi1 Mutation in thiamine...(mcrBC-hsdRMS-mrr) recA1 endA1 gyrA96 gal-thi-1 supE44 λ-relA1 Δ(lac-proAB)/F' proAB+lacIqZΔM15 Tn10 (...

  16. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    ... be your actual mentor? Is there a chance the supervisor will be moving on soon?   Will you get to meet...interviewing: Lab members speak disrespectfully of supervisors and/or each other. You are not allowed to speak... before answering questions about lab culture. Support for diverse career paths I hope by now you are ...making machine. Choose one that is a true mentor, supporting career development and growth. Have lab alumni...someone(s) train you technically? Will the PI/supervisor look at your data and suggest follow up experiments...

  17. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...corresponds to the supercoiled plasmid monomer, and at <6 kb, consistent with the size of a supercoiled plasmid ...sample. In these instances, please note that the supercoiled dimer band can sometimes overlap with the band...development to examine plasmid topologies (e.g. supercoiled, open circular, linearized, etc.) (Schleef et...Rudolph C. Transgene expression of transfected supercoiled plasmid DNA concatemers in mammalian cells. J...

  18. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ... and logistic support to increase the chances of success, but also nurture a supportive and protective...mentee, where (s)he feels truly understood and supported, and can more fully identify with and be inspired... Vela, who reiterated the Spanish government’s support for the IMP program as well as for the Spanish ...public and private institutions also showed their support and hope for the IMP program. The conference also...

  19. Adenovirus Guide

    Type
    Guide
    ...adenoviral genes have been removed. These genes are now supplied in trans , via different plasmids or the packaging...for viral replication and therefore needs to be supplied through other means. In order to produce rAdV ...One can also co-transform standard BJ5183 with supercoiled pAdEasy and the shuttle plasmid, but this method... . Journal of Genetic Syndromes & Gene Therapy, Suppl 5 , 001. https://doi.org/10.4172/2157-7412.s5-001...

  20. Molecular Biology Reference

    Type
    Guide
    ...gyrA462 endA Δ(sr1-recA) mcrB mrr hsdS20 (rB- mB-) supE44 ara14 galK2 lacY1 proA2 rpsL20(StrR) xyl5 lambda...lacZYA-argF) U169 recA1 endA1 hsdR17(rk-, mk+) phoA supE44 thi-1 gyrA96 relA1 tonA EPI300 LGC Biosearch Technologies...McrA-) recA1 endA1 gyrA96 thi-1 hsdR17(rK- mK+) supE44 relA1 Δ(lac- proAB) [F traΔ36 proAB lacIqZΔM15]...Invitrogen F- mcrB mrr hsdS20 (rB-, mB-) recA13 supE44 ara-14 galK2 lacY1 proA2 rpsL20 (StrR ) xyl-5 λ...
Showing: 61 - 80 of 456 results