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We narrowed to 447 results for: SUP

Showing: 61 - 80 of 447 results

  2. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...which we talk about below. Processing The viral supernatant is typically collected over the course of a couple...to 24 hours. Processing the virus-containing supernatant will depend on the virus type. AAV is typically...methods like ultracentrifugation, while lentivirus supernatants are centrifuged and filtered to eliminate any...than a P1 virus. Pro-tip! Centrifuge the viral supernatant in between passages to eliminate dead cells and...day or two, but unlike mammalian systems, the supernatant isn’t collected sequentially. This is because...majority of cells have died to collect your viral supernatant. Baculovirus replicates inside insect cells, ...ends in cell lysis, releasing the virus to the supernatant. The virus will reach peak production when roughly...

  3. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...t always indicate the presence of supportive data. To be supportive, the data must show that the antigen...This is the most usual level of evidence supplied to support the suitability of an antibody for its target... antibodies. It is also important to seek out supportive data on candidate antibodies from the manufacturer...a specific cytokine) known to either induce or suppress the expression of a target of interest, you can...However, the cell treatment will frequently induce/suppress the expression of many related proteins, not just...protein, show the same pattern of labeling, this is supportive of their specificity for the protein. A challenge...open science ecosystem. It has received in-kind support to produce this data from commercial antibody manufacturers...

  4. March for Science

    Type
    Blog Post
    ...hypothesis. The results of experiments can help support or reject a hypothesis. Looking at the data, scientists...trust in scientists if the march highlights data supporting unwanted results (14). Some loss of trust, I ...science) they come from many different places supporting many different positions. It would be detrimental...going change depending on the number of people supporting it.  3) Linking scientists to one particular ...world. There is no requirement that marchers or supporters be scientists – on the contrary, anyone and everyone...satellite marches. Anyone can join the marches or support the cause. For science to be integrated into politics...29). I tend to agree with him. Information on supporting scientists and science in government: 314 Action...

  5. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    ....   PS: The superhero race is the perfect event for supporting AJ, who is a big superhero fan. This year...Cambridge Super Hero 5K on October 23rd. This is a great event at which people dress up like superheroes or ... their power from the emotion HOPE; a fitting superpower for those running on behalf of rare disease sufferers... superhero 5k...

  6. Addgene: The First Twenty Years

    Type
    Blog Post
    ...arrived in 2012, our repository was rapidly able to support sharing of CRISPR constructs and information across...AAV vectors, as well as developing and sharing supportive educational resources. By 2017, we had distributed... the COVID-19 pandemic, and Addgene pivoted to support researchers combatting the virus. Our COVID-19 ...Addgene began to think about how we could expand our support for the research community once more. Our ready-to-use...another plasmid-based product that, with Addgene’s support, could fill a community need for high-quality service...Neuroscience AntiBody Open Resource (NABOR) platform, supported by an NIH BRAIN Initiative. We also created a .... And we look forward to twenty more years of supporting you in your work, your research, and your discoveries...

  7. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...which the required adenoviral helper genes are supplied on two different packaging plasmids: the pHelper...your cells, wait 2–5 days and then collect the supernatant and/or producer cells for further processing....you can consider using unpurified AAV from the supernatant (Goodwin et al., 2020) or crude cell lysate (...  Purification To purify your rAAV from your supernatant, you can simply use polyethylene glycol (PEG)... 25 mL of 40% PEG solution to each 100 mL of supernatant (final concentration of 8%). If, however, you...sonication.  The obtained virions from cells and supernatant then need to be purified by density gradient ...particles containing a viral genome. While ddPCR is superior in precision and variability, qPCR is the more...

  8. Plasmids for Stem Cell Research

    Type
    Collection
    ...Dermal Fibroblasts Neurons Lentiviral Human REST suppression mediates neural conversion of adult human fibroblasts...

  9. Networking and Inspiring Young Scientists at Freshers Fairs

    Type
    Blog Post
    ...benefit from some Addgene support? Please get in touch, as we aim to support a couple of events a year...undergraduates and PhD students so that they can help support each other and provide advice. The society organizes...involved with. The society provides talks and career support for a range of different scientific disciplines...alumni to mentor younger students in society and to support iGEM alumni-industry networking to promote the ... able to help with every request, we do aim to support events and scientific community activities throughout...

  10. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...10 that suppress APCs. T cells that would normally attack the cancer cells are also suppressed by chemical...immune cells and cells of epithelial origin. Suppressing function, creating tolerance, and evading recognition...by which tumors sidestep immune surveillance: Suppressing Function Antigen presenting cells (APCs) that...motif chemokine 22 (CCL22) and TGFβ. T-regs are suppressive immune cells that restrict the production and... change CD4+ Th cells into regulatory cells to suppress function. Creating Tolerance T-regs in the tumor...combination of tolerance marker blockade and suppression of tumor-aiding cytokines, for example, will ...

  11. Which Fluorescence Microscopy Techniques is Best for Me?

    Type
    Blog Post
    ...nanometers of the glass/water interface. Super resolution microscopy Super Resolution Microscopy allows for imaging...matter whether you are a sports photographer at the Super Bowl, a medical technologist taking an x-ray, or...spatial contrast (i.e. optical sectioning (2) or super-resolution (3). Fluorescence microscopy techniques...higher signal to noise ratio can be achieved (6). Super-resolution techniques such as Structured Illumination... these types of samples. Prior to the advent of super-resolution imaging, the light microscope was limited... appear as a fuzzy 200-300 nm spot when imaged. Super-resolution microscopy uses a number of optical and...

  12. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ... people who will support your professional development I had a wonderfully supportive mentor and graduate...they had my back. If your advisors are not as supportive of your career goals, find people who are. I ...mentioned, my graduate office staff was amazingly supportive. I talked to them about my interests and my ideas...professional organizations, and other nonprofits to gain support for CINSO. For instance, I tweeted to the Indiana...ever since, the State Museum has been the best supporter and most productive partnership CINSO has had....

  13. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    ...Singletons together would provide a structure and support network for people attending large meetings. A ...those who could not participate but provided moral support was invaluable.  Mixing metaphors and scientists...societies and conferences should strive to provide support to early career scientists, students, and those...ALL their members.  Shoutout to our mentors and supporters Thanks to Dr. Amanda L. Gunn  and Dr. Denise ...Singletons is especially grateful to its mentors and supporters during this first year. Mentors Amanda Gunn, ..., and Pragati Chengappa.  Financial and moral supporters Denise Akob, Sara Alexander,  Jill Bradley, Taylor...

  14. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...the scientific support team at Synthego. Her work centers around educating and supporting novice to expert-level...was contributed by Jessica Roginsky, Scientific Support Lead at Synthego. Article source: Step-by-Step ...this problem. This tool was initially created to support the CRISPR analysis needs of Synthego’s scientists...other features and functionalities, please email support@synthego.com. Overview of ICE editing analysis ...CRISPR Gene Knockout Kit. Please contact us at support@synthego.com if you have any questions, and check...

  15. Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

    Type
    Blog Post
    ...scientists. Many members of the scientific community support open sharing. But, one critical part was missing...universities are showing an unprecedented amount of support about allowing use of plasmids for research by ...weeks. As of June 2020, we are happy to have the support of the following organizations to share materials... synthesized approximately 3,000 constructs to support SARS-CoV-2 research.  Browse the entire Ginkgo ...and funding initiatives such as Fast Grants that support us in our mission.  While rapidly expanding our...

  16. Scientific Sharing in the Time of COVID-19: Databases and Resources

    Type
    Blog Post
    ... world. To support this pace of research, new, centralized databases and centers to support the sharing...like here at Addgene!), and new databases are supporting their access to useful resources during this ...research Universities have also been stepping up to support COVID-19 research. The new Center for COVID-19 ...Preparedness (CCRP2) at Rutgers University not only supports collaborations at Rutgers, but also lists data...

  17. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

    Type
    Blog Post
    ... Accelerating science and supporting scientists globally are the shared mission of both Seeding Labs ...well as developing yeast as source for dietary supplements! Development of a bacterial expression system...plants.     Transgenic yeast as source of dietary supplements Dr. Mrinalini Nair, University of Baroda, India...proteins in order to manage insect pests and to support sustainable agriculture. For this project the expression...in Mexico. At Addgene we're happy to be able to support diverse groups of scientists working in a wide ...

  18. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    ...lifetime supply for about $10 US dollars worth of glass powder pruchased from a pottery supply house.  ...either.  Test tubes aren’t cheap everywhere Lab supplies and equipment are more precious in less resourced...Labs does an amazing job shuttling unwanted lab supplies to places that really need them.  When I was in...stop me. I only had to replenish my glass milk supplies every 10 years or so. Actually, I was happy to...

  19. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    ...and have always been, a community resource that supports both toolmakers and tool users. We encourage you...Educational Resources As part of our commitment to supporting your research, Addgene is creating an array of...Through our incredible community, including your support, we have enabled scientists to easily share plasmids...Studies Hybridoma Bank, a nonprofit that has been supporting hybridoma sharing within the community for decades...Immunofluorescence Addgene’s Antibody Guide The project is supported by a NIH BRAIN Initiative grant (U24NS119916 )...

  20. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...PA-FPs have helped to revolutionize the field of super resolution microscopy. PA-FPs, especially the monomeric...monomeric, reversible type, are very well suited for super resolution imaging, where the power of the technique... reconstruct an image. The best PA-FPs used for super-resolution imaging usually have the following characteristics-high...fluorescence wavelengths. Indeed, within the world of super resolution microscopy, PA-FPs have helped create...are vastly improved over the conventional FPs for super-resolution imaging, they still do not emit the large...being engineered. By combining PA-FPS with superresolution microscopy, scientists now have at their fingertips...
Showing: 61 - 80 of 447 results