...structural proteins expressed from a reverse-transcribed piece of DNA called a “provirus.” This DNA is...proteins because they integrate this reverse-transcribed DNA into the host cell’s genome and then use ...is a turn of phrase — “pseudotransduction” — to describe the direct protein transfer of RT, which would...

...from Alex Schier’s Lab. gRNAs can be in vitro transcribed from ssDNA, which can be generated by commercial...targeted approach using RNPs has recently been described in vitro, with the hope of eventually using this...PMC5681539. Additional resources on the Addgene Blog Subscribe to CRISPR blog posts Learn about other CRISPR ...

...used in research, and data repositories. Below I describe how each of these kinds of connectivity facilitates...deposits will be made. The Dataverse integration is described in this video and the Figshare integration in ...from RSpace documents to external data sources (described above) further enhances the value the repository...

...restriction endonucleases in plasmid addition was first described by studies of EcoRI and Bsp6I in Escherichia coli...systems. As with the other toxin-antitoxin systems described above, one part of the pair is more stable than...toxin-antitoxin system discovered. Unlike the systems just described, the hok/sok systems consist of three components...

...designed, each binding to one strand of the reverse-transcribed target sequence that are present close to the...amplified by PCR using Taq Polymerase and then transcribed to provide RNA substrates for Cas13. Cas13, upon...fluorescence upon cleavage. Highlights: Reverse-transcribed RNA samples are amplified by PCR instead of RPA...

...genome editing with CRISPR/Cas9 in human cells was described in 2013 (Cong et al, Mai et al) we were very excited...thinking further about the distance relationship described above, we realized that, in addition to increasing... and neurovascular diseases. He worked on the described project while he was a Postdoc in Marc Tessier-Lavigne...

...site-specific incorporation of phosphoserine (as described below) provides researchers with a valuable tool...context-specific effects of UAG suppression, as described (6). To determine the stoichiometry of phospho-incorporated...phospho:non-phospho species on PhosTag gels as described above. Reagents and protocols Sep-OTSλ plasmid...

...promoters can be used in the repressible manner described above, as well as the inducible manner detailed...30 years since the original Tet-Off system was described by Gossen and Bujard, both the promoter and transactivator...cells, and can be used in the repressible manner described above, as well as the inducible manner detailed...

... been described in this blog. If your data does not meet the assumptions for the tests described above...error rate than the multiple comparison tests described above. Violated assumptions and statistics Statistical...

...inserted in this LTR so you don’t need Tat to transcribe the viral genome in producing cells. Currently...process is to test several protocols that have been described (protocol 1, protocol 2, protocol 3) to find the...quantify viral RNA. All of these methods have been described in a previous Addgene blog Once you know your ...

...Plasmid #52962) was a gift from Feng Zhang and is described in Improved vectors and genome-wide libraries ...was a gift from John Doench & David Root and is described in Rational design of highly active sgRNAs for...were gifts from John Doench & David Root and are described in Optimized sgRNA design to maximize activity...

...system is much more potent than the in vitro-transcribed single guide RNA (IVT sgRNA) approach (Dewari...comparison of Cas9 RNPs prepared with an in vitro-transcribed (IVT) sgRNA versus synthetic cr:tracrRNA. Cells...step by step protocol for the csRNP method is described in our recent article (Dewari et al. 2018), however...

...after the piñata burst, value was subjectively ascribed relative to the basic needs of each individual...There are 8 major categories found in the table, describing everything from basic to advanced functionality...Software design elements that fall into this category describe how the user is expected to interact with the ...

...in practice integration can occur. Okita et al describes a protocol for generating iPSCs by plasmid transfection...Methods RNA Delivery: For this approach, in vitro transcribed RNAs are serially transfected using a cationic...transduction, such as the polyarginine peptide described in Zhou et al and Kim et al. This is considered...

...an alternative method, called STARR-seq (self-transcribing active regulatory region sequencing) that tests...assay. In their recent publication, Kroeze et al. describe their enhanced TANGO arrestin recruitment assay...When used in combination with their previously described SpyTag/SpyCatcher pair, multiple protein fusions...to fuse the AID tag to endogenous proteins. As described in their recent Cell Reports paper, the Kanemaki...

...your experiment* Functional data, if the report describes a functional vector such as a channelrhodopsin...site as well as the target region, if the report describes a retrograde serotype An abstract that summarizes...

...researchers at Japan’s Hiroshima University who described their new tool in Scientific Reports in June. ... gap. As Sakuma and colleague Takashi Yamamoto describe in their recent study, the construction system...

...characteristic radiance for some time – there are records describing them dating back to the first century A.D. – ...mechanistic understanding of GFP’s function. His lab described the protein’s structure and also discovered many...

...This protocol describes how to generate a monoclonal cell line from a polyclonal pool of stable cells...Protocols Viral Service Introduction This protocol describes how to generate a monoclonal cell line from a ...type will be able to grow under the conditions described in this protocol. Conditioned medium (details ... specific conditions used. Each 96-well plate described in this protocol could, in theory, give rise to...protocol was performed at Addgene on the A549 cells described in our sample data , each 96-well plate gave rise...from Feng Zhang (Addgene plasmid #52962 ) and is described in Improved vectors and genome-wide libraries ...

...supporting the tools in our collection, such as blogs describing viral vector serotypes, or hundreds of Data Hub... to learn more about our latest developments? Subscribe to the Addgene newsletter. References Ayoubi,...