Skip to main content
Addgene
Showing: 61 - 80 of 562 results
  1. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...Mouse IgG2a 177521 Anti-TARPGamma2/Stargazin [N245/1R] TARPGamma2/Stargazin Rat Mouse IgG2a 177522 Anti-TARPGamma2...
  2. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    ...scientist started with the Addgene blog. I wrote my first blog post out of boredom. I had just started working...wondering how to get started. This blog post outlines 10 steps to take when starting to use Twitter as a...Before I started writing for the Addgene blog, sharing Chemistry Cat memes was how I used social media...social media professionally, and 2) I needed to start using Twitter. Sharing Chemistry Cat memes use...science It may have taken a bit of FOMO to get me started, but now I’m using social media to help my research...to do with it. Five resources to help you get started with social media as a scientist Using social media...it’s hard to know where to begin. To help others start using social media to their professional advantage...
  3. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    ...for you, don’t wait. Start communicating now. These 9 strategies will get you started:  Subscribe to Career...Take the DIY approach and start a blog. Stellar talents like Ed Yong jumpstarted their science writing career...Twitter account. Check out the hashtag #scicomm and start following science communication societies like @..., set up a blog using a site like WordPress and start writing. Grow your audience by advertising your ...are open to guest contributions. If you decide to start your own blog, check out these two excellent articles...career. The moment, I got active with it, new doors started opening up. My current job as a Marie Curie Fellow...Fellow is a direct consequence of a blog that I started writing three years ago on a field trip in Indonesia...
  4. 10 Ways to Share Your Science!

    Type
    Blog Post
    ...office is a good place to start. It’s not unusual to find science writers who started out as graduate students...skills. So, where can a graduate student or post-doc start? The good news is there are more opportunities than...Here are my recommendations on some ways to get started sharing your science with the community. Some of...father or your best friend who isn’t a scientist, start. If they don’t seem all that interested in the details... You’ll know you are on the right track if they start asking you questions. Remember that people like ...written by practicing scientists. 3)   Consider starting up your own twitter feed or talking about and ... writing and are ready for a larger commitment, start your own blog. Plan to write regularly and, when...
  5. How to Write a Scientific Review Article

    Type
    Blog Post
    ...discussing the review topic with your peers. Start reading! I started by reading other reviews because, as I...from a wide range of authors. Just start writing When I first started I thought I would read a bunch of... scientific review.” The second thing I did was stare in horror at the limited number of hits. So, here...outline the organization of the review Once you start reading, there will be a temptation to include every...normal day.” The way I broke this cycle was to just start writing. A word of advice: when reading, don’t feel... feel like you need to read every paper from start to finish. Find the information that’s relevant to ...results and contextualized them. No really, just start writing! Don’t worry about grammar or formatting...
  6. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...connections to the starter cell, RABV was modified to only infect user-defined starter cell types, as well...’s architecture and plumbing are important. We started with learning the head is connected to the neck...the whole brain, it first infects one cell (the “starter cell”). It then replicates within that cell, and...retrogradely infect neurons that project to the starter cell. Once this first order infection occurs, subsequent...). Based on this, cell type specificity of the starter cell can be achieved by using EnvA-pseudotyped ...selectively supplying the G protein in trans only to starter cells (infected with G-deleted RABV). In this case... case, G-deleted RABV will infect the starter cell. RABV replication will then occur and the G protein...
  7. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ...always a good idea to start close to your comfort zone and slowly expand from there. Start small: Collaboration...my graduate studies at Karolinska Institutet I started working with other groups. First within my department...example, I worked with some microscopy enthusiasts to start a microscopy seminar to connect the imaging community... scientific collaboration - show initiative and start organizing meetings and take minutes. Get to know... little in common with you is much harder than starting a collaboration with a colleague. However, reaching...discussing with strangers stopped feeling weird and started to be fun. I just had to remember to approach the...the one that you feel enthusiastic about and get started. You never know where the relationships you develop...
  8. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    ...searching. Start your career planning early, start now Don't wait until the day you defend to start thinking...thinking about your next step. Start thinking now. Start browsing job postings and look at the jobs you are...job postings and salary expectations public, so start by looking at these university postings to give ...in academia or not, it is even more important to start early. Think about your plan for the next 5 years...Here are some great resources to get your resume started now. Good luck! Resume 101 from University of ...
  9. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ...academic scientists are starting to see the advantages of using social networking to start, build, and track...tell when some start-ups were about to be bought by seeing certain groups of people start to update their...my seven best tips beyond the basics to get you started. 1) Be visible and open to connections This is...add a personal note, you waste an opportunity to start things out right. Also, many people on LinkedIn ...include news, inclusion, culture and connections. Start your interview research there! 6) Your LinkedIn ...changes. If you are working, and all of a sudden you start updating your profile, everyone will think you are...
  10. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

    Type
    Blog Post
    ...Center. Susan Keown’s science communication journey started with something unexpected: termites. “I was really...says Susan. Her passion for insects lead her to start a PhD in entomology at the University of Maryland...know if she liked science writing. She decided to start a blog to test it out and discovered she not only...continued working for Palladian Partners remotely, but started looking for a new job because “it kinda sucked ...hired a full-time social media manager. “I usually start out my days looking through our Twitter feeds and...Susan’s number one piece of advice for getting started in science communication: “Find as many opportunities...post-grad school individuals, Susan recommends starting a blog, but with one caveat: “I hate telling people...
  11. Finding Your Science Policy Path

    Type
    Blog Post
    ...for science policy folks is on Twitter- start an account and start engaging with the #scipol hashtag. Often... to college to graduate school, standing at the start of a science policy career was much more circuitous....  Who does science policy? I’ve intentionally started with the ‘who’ question because the science policy... from your alumni community is a great place to start. What is science policy? THE IMPOSSIBLE QUESTION...tenured professors. There’s never a bad time to start your science policy journey.  In fact, working in...time and that’s perfectly normal!  Action Items:  Start a career journal during your current job and note...this crowdsourced list of foundational texts as a starting point.  Assess and develop your own science policy...
  12. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ...Pivot Back in March 2020, just four months after I started with Addgene, I was about to have a check-in with...resources we have, and we developed a great plan to start consistently knocking out high-quality videos for...the window. So, much like SynBio, it was time to start our Design-Build-Test-Learn cycles. If you’re not...space to shoot was another hurdle. As we slowly started to realize that we were going to be out of the ...wasn’t going to work for our other videos. For starters, I hadn’t anticipated nearly every webcam recording..., but still have control over focus, zoom, stop/start record, and allow me to see an image.  Remote video...camera, to control some basic functions, including starting and stopping recording).  Test: It was unbelievable...
  13. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    ... are a few out-of-the-box ideas that might jump start your learning. 1. Create a peer mentoring group ... career and life goals. For tips on how to get started see Form Your Own Peer Mentoring Group: A How-To...for the live audiences in your future.     3. Start something In almost every career talk I present,...charge before others do”. Candidates who have “started something” which they can share on their resumes... of things: led a grad or postdoc association, started a speaker series or biotech club, managed their...ukelele...perhaps you get the idea. What will you start? 4. Do informational interviews (on a schedule) ...you can’t wait until you imminently need a job. Start now. Informational interviews are 1:1 discussions...
  14. Design Tips for Prime Editing

    Type
    Blog Post
    ... are a great starting point: Test different lengths of the primer binding site, starting with a length...General principles pegRNA design If you’re just starting out with prime editing, you’ll need to design ...different lengths of reverse transcriptase template, starting with about 10–16 nucleotides. For longer templates...editing (Anzalone et al., 2019). Figure 2: Starting lengths for optimizing your pegRNA primer binding...template during DNA repair. Test multiple nick sites, starting with sites about 50 bp upstream and downstream...editors bound to sgRNAs only drive nicking (right). Start with nicking sgRNAs ~50 bp from the prime editing...
  15. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ...you need to start a whole new blot? Never fear: membrane stripping is here! Rather than start over, you...whole new gel and membrane transfer. Let’s get started! Before you begin In the best case, you know ahead...stripping and reprobing, maximize your signal by starting with the least-abundant protein and working toward...antibodies will require harsher stripping to remove, so start with the weaker binding ones first to preserve your... loss. If you are still optimizing your system, start with a mild stripping solution and move onto a stringent...incubation times, or try the stringent solution if you started with the mild one. Reprobe Once your membrane is...
  16. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...time PCR is a PCR technique used for measuring a starting DNA concentration using PCR. qPCR requires the... cycle.  To determine the concentration of the starting template DNA the fluorescent signal throughout...to a standard curve of amplified DNA of a known starting concentration. The cycle in which the unknown ...standard curve can be used to determine the amount of starting material in your sample.  qPCR can also be used...qPCR to quantify the concentrations of multiple starting DNA templates in one sample. It is important to...mutagenesis Molecular Diagnostics qPCR Quantify starting template DNA concentration Gene expression ...ultrasensitive and absolute nucleotide concentration of starting material AAV Titrations Allelic Variations Quantifying...
  17. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    ...all the basic and necessary elements in order to start using the MoClo system. The paper also gives quantitative...have any particular tips for those looking to get started with MoClo? Patron: The parts will be familiar ...the parts that you need from the kit and getting started making your own constructs with Golden Gate MoClo...conceptual barrier. Sometimes people don’t know how to start. They might want to use our promoters and terminators...they don’t have any prejudice about it. They just start and go ahead. I can see science speeding up. Addgene...engaging about science in that way for you? Patron: I started my Twitter account recently inspired by an academic...ate plants too. Think about how many people are starving. I think plant biology is massively underfunded...
  18. Michael Koeris' Journey from Grad Student to Entrepreneur: The Story of Sample 6

    Type
    Blog Post
    ...this idea, Drs. Koeris and Lu founded the biotech startup company, Novophage. As you'll hear Dr. Koeris explain... decided to develop phage for use in a biotech startup 12:16 - 15:15 : Why Sample 6 began to develop tests...Koeris to personally take on the risk of founding a startup company? 32:20 - 34:37 : Whose responsibility is...Sample 6 website Check out Dr. Koeris' Website, biotechstart.org Read Sample 6's Paper on Bacteriophage-based...Other Posts in Our Podcast Series Get Additional Startup Advice Learn How to Be A Good Manager from Addgene... startup...
Showing: 61 - 80 of 562 results