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We narrowed to 616 results for: TIL

Showing: 61 - 80 of 616 results

  1. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    ...the mandate has been removed, institutions should still go ahead with raises, as for years there have been...planning to raise salaries prior to the injunction are still honoring these plans. We are now collecting data...following the injunction, 59% of all postdocs are still expected to receive salary raises - down from the...to get a salary increase prior to the injunction still won’t after the injunction (see below). Of the ...salaries, the only postdocs we knew for certain to still be getting their raise were: postdocs at institutions...University of Louisville in Kentucky, the raises are still going ahead as planned. As we are receiving data...

  3. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ...from one cell to another). Bacteria need special fertility genes to be capable of conjugation, and these ...penicillin resistance plasmid can grow in spite of a hostile Penicillium fungus trying to kill them off. While...possess plasmid-borne toxins like colicins and subtilisins to kill other bacteria in the area, freeing up...systems that allow tuning of metabolic pathways. Fertility (F) plasmids: microbial sharing F plasmids contain...shifted over the years, and new classifications are still being created as whole-genome sequencing becomes...Gateway cloning relies on genes found on an E. coli fertility plasmid that also encodes a toxin, CcdB, and the...

  4. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...The versatility of CRISPR allows you to play with DNA in a number of ways, from small edits that change...seemingly ubiquitous in nature, they are largely underutilized in research settings — mostly a consequence ...Cas3 — a tactic some CRISPR transposase systems utilize with type I-F Cascades. Figure 2: Cascade...plasmids — such as the Tomoji Mashimo lab and the Tilmann Weber lab — to increase efficiency of these systems...systems, though consistent expression can still be a challenge. A multitasking Cas Most Cas effectors have... potential. Hopefully more people will want to utilize Cas3’s degradative power in the future! References...

  5. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ...introduced into the B. subtilis genome. Mobile-CRISPRi is then transferred from B. subtilis to the bacterium...CRISPRi into a single bacterial strain, such as a B. subtilis, or a narrow range of bacterial species, such ...integrative and conjugative elements in Bacillus subtilis (ICEBs1) system. ICEBs1 carries the genes needed...bi-parental mating. Following excision from the B. subtilis genome, the Mobile-CRISPRi ICE element forms a...integrative and conjugative element in Bacillus subtilis." Plasmid 86 (2016): 14-25. PubMed PMID: 27381852...

  6. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...c-terminal nuclease domain. Feng Zhang's group utilized the bi-lobed architecture of Cas9 to engineer ...activation Optogenetics is a powerful tool that utilizes light to control and monitor individual living...CRISPR-Cas9 system to create a tool that is quick, versatile, and robust. Dubbed the LACE system for light-...signalling pathways. These LIDs are generalizable, versatile and powerful tools which allow you to easily and... both live cells and whole animals. The system utilizes the bulldozer-like quality of the ribosome during...the hypothesis that most mRNAs are not translated until they are exported to the cytoplasm. Furthermore,...sgRNAs with Cas9 for genome engineering. The versatility and ease of generating complex combinations of...

  7. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    ...refers to as “PAM deserts” in the genome. More versatile and precise than base editing (in certain circumstances...application, and the efficacy varies widely — it still almost always results in higher ratios of desired...Prime editing tools Prime editing is precise and versatile, but it requires careful optimization to achieve...cost of improved efficiency, although both enzymes still approach or exceed PEmax editing efficiencies for...obtain PEs small enough to deliver with AAVs but still efficient at long and complex edits. The team also...this tail is degraded, the truncated pegRNA can still guide Cas9 and compete with full pegRNAs for access...engineered virus-like particles. However, there’s still a long way to go before we see prime editors in ...

  8. Evolution of Lab Techniques

    Type
    Blog Post
    ...saving the researcher both time and resources (3). Still, researchers generating transgenic animals may choose...helped researchers at a time when sequencing was still in the early days of development. Today, researchers...restriction enzymes are certainly not dead yet! Labs still keep restriction enzymes on hand for cloning and...sequencing (more on that below), restriction mapping may still be used to screen multiple colonies before selecting...expensive sequencing fuels a sequencing revolution that still prevails. Sequencing whole genomes or profiling ...techniques have largely replaced these, these techniques still have useful applications and have informed many ...

  9. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...Transformation Summary PCR based cloning is incredibly versatile and allows for nearly any piece of DNA to be placed...colonies are a result of uncut empty plasmid, you will still have colonies when you do not add ligase. If the...

  11. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...study their function in health and disease, but, until recently, it just wasn’t that easy. In the 1980s...was fascinated by CRISPR and rushed to test it. I still remember the day, March 16, 2014, that I got my ...into either the pronucleus or the cytoplasm of fertilized mouse eggs. Or, to avoid handling embryos ex ...that you can inject the reagents directly into fertilized mouse eggs, circumventing the need for enrichment...transmission of the transgene, this technique is still in need of improvement. Also when working with CRISPR... discovered in an obscure bacterium and that we still have much to learn from biology in all its forms...

  12. Important Considerations When Using AAVs

    Type
    Blog Post
    ... the University of Manitoba. Our lab has been utilizing chemogenetics (Designer Receptors Exclusively ...themselves are not pathogenic, precautions should still be taken when using them. There are suspected cases... useful for a wide variety of experiments, care still needs to be taken when selecting which particular... other viruses, multiple freeze thaw cycles can still reduce AAV titer. For long term storage, make > ... vs 7 days vs 14 days etc. To decrease the time until expression, it is possible to use AAV constructs...Additional Resources on the Addgene Blog AAV: A Versatile Viral Tool for Gene Expression in Mammals A Match...

  13. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...fluorescent sensors. Retroviral vector background Until the development of viral vectors, gene transfer ...membrane pores) transfection. These techniques are still used and efficiently transfer plasmid DNA efficiently...vector. Lentiviral vectors Although retroviruses are still in use, considerable efforts have been devoted to...However, in this vector production system, there is still a potential risk for the generation of replication...however improving the safety of these vectors is still an active area of research due to the possibility...Replication-incompetent lentiviruses with human tropism can still infect the researcher and insert into her/his genome...

  14. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

    Type
    Blog Post
    ... NpHR, can even be expressed simultaneously and still be individually activated with different lights ...assays can be done on zebrafish at 5 days post fertilization, including escape behavior and spontaneous swimming...swimming. When startled by acoustic, visual or tactile stimuli, larval zebrafish react by performing a...immobilized in agar at the head with their tails still free, and different regions of the spinal cord were... offered by the optogenetics toolbox and the versatility of the zebrafish model organism, the Isacoff ...

  15. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ...field, let's look at how prokaryotes originally utilized CRISPR/Cas as a powerful and adaptive defense ...safeguard against bacteriophages did not come to light until 2007. Initial experiments exposed S. thermophilus...prokaryotes developed an adaptive immune system that utilized various cas genes not only to store a record of..., 83% of archaeal and 45% of bacterial genomes utilize this system (4). Makarova et al. grouped the systems... not translatable to the other CRISPR subtypes. Still, the large and dynamic range of CRISPR systems between...

  16. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ... question of what I wanted to do when I grew up until I was all but finished my undergraduate degree (...of how to appeal to a given audience and how to utilize a wide array of media in doing so—can prove invaluable...of science communication, then, is its sheer versatility in the face of disparate spheres of research....incorporate other facets which occupied my attention, until these coalesced into a research topic on concussion...unsettled science. Understanding just how the public utilizes insights gleaned from scientific research is, ...

  17. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    ...generally agreed-upon international moratorium on utilizing CRISPR/Cas9 in humans. This topic was to be revisited...than having a meeting to agree upon a moratorium until another meeting, three branches – a private funder... unethical experimentation on human subjects, utilizing a scientific technique not proven adequately safe... of the consulting firm Rogue Bioethics. Kelly utilizes her firsthand experience in the software industry...all of whom are named Charlotte, all of whom are still working on theirpenmanship. Additional resources...

  18. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    ...think I would have had the nerve to do what I did. Until that panicked, split-second decision, I had always...mind went blank, my options were to stop talking until I could present something coherent or to quickly...related to this paper and/or biology in general until I reached Fig. 6, Panel L, and hope nobody noticed... take that step. Repeat that as often as needed until you get to the end of the presentation, at which... to be anxious, nervous, and/or apprehensive. I still get nervous quite regularly, and I've had multiple...

  19. RNA Extraction Without A Kit

    Type
    Blog Post
    ...extraction methods evolved into a simple protocol still used today There are many alternative methods for...-phenol-chloroform. In this method, samples are still homogenized and lysed in a guanidinium thiocyanate...hours, and variations on this no-kit method are still widely used today (Chomcynski and Sacchi, 2006)....Making the simple protocol even more foolproof (still without a kit!) As mentioned above, working with...with RNA requires keeping your samples cold until homogenization and cell lysis. This can be challenging...

  20. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type
    Blog Post
    ... and following citations through the literature until you have all of the information you need. Reading..., but wait to sort out any of the finer details until you see the figures in the results section. As you...experiments are geared towards eliminating possibilities until the results focus in on a particular conclusion.... you prefer, printing and hand-writing notes is still possible, but not feasible for every paper you will...search. Writing this tutorial has shown me that I’m still in the dark ages with respect to my organization...
Showing: 61 - 80 of 616 results