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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 935 results for: TIM

Showing: 61 - 80 of 935 results

  1. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...target-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and Cas...

  2. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...target-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and Cas...

  3. Institute for Protein Innovation

    Type
    Collection
    ...technology (Link opens in a new window) and an optimized sorting process. This combination allows IPI to...

  5. Resolute Plasmid Collection

    Type
    Collection
    ...Plasmids This plasmid collection contains codon-optimized ORF sequences for human SLCs in Gateway-compatible...

  6. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type
    Blog Post
    ...accumulated over time? Undergraduates can help to answer this question! The Pathways Over Time project The...The Pathways over Time project provides a framework for these undergraduate research questions. The idea...from a common ancestor ~350 million years ago. Over time, the two genomes have become very different due ...species diverged. Tips on using the Pathways Over Time plasmids The plasmids are used as part of a semester-long...D.M., Ludlow, L.H., & O'Connor, C.M. Pathways over time: Functional genomics research in an introductory... pathways over time...

  7. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    ...only easily identify base editing events in real-time but also enrich for base-edited cells in their experiments...Monitoring APOBEC and Cas9-mediated editing in real time The Harris lab created ACE, a reporter that monitors...deaminase proteins) and Cas9-mediated editing in real time (St. Martin et al., 2018). The ACE reporter is a...canonical base excision repair enzymes. At the same time Cas9n cleaves the opposing DNA strain which results...reporter quantifies APOBEC-Cas9 efficiency in real-time, it relies on DSBs which is not ideal for monitoring...efficiency of APOBEC-Cas9 editisome complexes in real-time (St. Martin et al., 2019). To create the eGFP reporters...sequencing (Standage-Beier et al. 2019). TREE is a real-time, fluorescent based identification system for the...

  8. Mammalian RNAi Tools

    Type
    Collection
    ... Web Resources Trono Lab Lentivectors Toolbox Optimized shRNA design in pSico with pSicoligomaker (Andrea...

  9. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...Cas9’s. Further advancements have been made by optimizing expression of the fusions, modifying the linker...

  10. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...target-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and Cas...

  12. Synthetic Biology - Overview

    Type
    Collection
    ...Taek Soon Lee Joshua Leonard Han Lim Wendell Lim Timothy Lu Michel Maharbiz Thorsten Mascher Kathleen Matthews...

  13. Antibody Production

    Type
    Collection
    ...generated using standard methods individually optimized for each specific antibody in order to generate...

  14. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...target-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and Cas...

  15. CRISPR Plasmids - Yeast

    Type
    Collection
    ...target-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and Cas...

  16. AAV Molecular Tools

    Type
    Collection
    ...CMV-driven, constitutive Expression of human codon-optimized Cas9 from Staphylococcus aureus (SaCas9). 6 Zhang...

  17. Viral Vector Guides and Plasmids

    Type
    Collection
    ... production plasmids that have been carefully optimized for both safety and efficiency. Learn more about...

  18. Distribution to Industry

    Type
    Collection
    ... variety of cell types. Protein Purification Optimization Kit Using SUMO Protease Elution Protein Characterization...

  20. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    ... pLKO.1 cloning protocol is viewed around 3,000 times each month. I looked this up after trying out the... problem with this is that it may take months of time for your manuscript to get reviewed and edited prior... a protocol as you finish them, turn on pre-set timers for incubation steps, and save the entire process...
Showing: 61 - 80 of 935 results