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We narrowed to 939 results for: TIM

Showing: 61 - 80 of 939 results

  1. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    ...this adds up to a significant time savings. You can also save yourself time if you're transforming a plasmid... each week at Addgene, so we need to be cost and time efficient. We do this by making our own competent...antibiotics to give the antibiotic resistance gene time to be expressed.  However, you can skip this incubation...

  2. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...target-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and Cas...

  3. Institute for Protein Innovation

    Type
    Collection
    ...technology (Link opens in a new window) and an optimized sorting process. This combination allows IPI to...

  4. Resolute Plasmid Collection

    Type
    Collection
    ...Plasmids This plasmid collection contains codon-optimized ORF sequences for human SLCs in Gateway-compatible...

  5. Mammalian RNAi Tools

    Type
    Collection
    ... Web Resources Trono Lab Lentivectors Toolbox Optimized shRNA design in pSico with pSicoligomaker (Andrea...

  6. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...Cas9’s. Further advancements have been made by optimizing expression of the fusions, modifying the linker...

  7. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...target-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and Cas...

  9. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    ...only easily identify base editing events in real-time but also enrich for base-edited cells in their experiments...Monitoring APOBEC and Cas9-mediated editing in real time The Harris lab created ACE, a reporter that monitors...deaminase proteins) and Cas9-mediated editing in real time (St. Martin et al., 2018). The ACE reporter is a...canonical base excision repair enzymes. At the same time Cas9n cleaves the opposing DNA strain which results...reporter quantifies APOBEC-Cas9 efficiency in real-time, it relies on DSBs which is not ideal for monitoring...efficiency of APOBEC-Cas9 editisome complexes in real-time (St. Martin et al., 2019). To create the eGFP reporters...sequencing (Standage-Beier et al. 2019). TREE is a real-time, fluorescent based identification system for the...

  10. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type
    Blog Post
    ...accumulated over time? Undergraduates can help to answer this question! The Pathways Over Time project The...The Pathways over Time project provides a framework for these undergraduate research questions. The idea...from a common ancestor ~350 million years ago. Over time, the two genomes have become very different due ...species diverged. Tips on using the Pathways Over Time plasmids The plasmids are used as part of a semester-long...D.M., Ludlow, L.H., & O'Connor, C.M. Pathways over time: Functional genomics research in an introductory... pathways over time...

  11. Synthetic Biology - Overview

    Type
    Collection
    ...Taek Soon Lee Joshua Leonard Han Lim Wendell Lim Timothy Lu Michel Maharbiz Thorsten Mascher Kathleen Matthews...

  12. Antibody Production

    Type
    Collection
    ...generated using standard methods individually optimized for each specific antibody in order to generate...

  13. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...target-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and Cas...

  14. CRISPR Plasmids - Yeast

    Type
    Collection
    ...target-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and Cas...

  15. Viral Vector Guides and Plasmids

    Type
    Collection
    ... production plasmids that have been carefully optimized for both safety and efficiency. Learn more about...

  16. Distribution to Industry

    Type
    Collection
    ... variety of cell types. Protein Purification Optimization Kit Using SUMO Protease Elution Protein Characterization...

  17. AAV Molecular Tools

    Type
    Collection
    ...CMV-driven, constitutive Expression of human codon-optimized Cas9 from Staphylococcus aureus (SaCas9). 6, 8...

  19. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...target-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and Cas...

  20. TALEN Guide

    Type
    Collection
    ...acids, their repeat-variable di-residue (RVD). Ultimately, it is this RVD that determines which single ...
Showing: 61 - 80 of 939 results