...mutant clones from watermelon have been codon-optimized for expression in bacteria and cloned into an ...

...proteins are expressed as both monomeric baits and multimerised enzyme-tagged preys. Ectodomain inserts: The...

... Working with plants doesn’t always have to be a time-consuming process. While developing transgenic hairy...transformation process could save the plant biologist some time… months, in fact. In the Lee-Parsons lab, we are...plant, the Catharanthus roseus (Figure 1). The ultimate goal of our research is to enhance their production...C. roseus by working with young seedlings and optimizing several other aspects of the transient transformation...available for your species This will save you a lot of time. Don't re-invent the wheel. Test different leaf ...

...localization changes over time.Hallmarks of fluorescent protein timers When FP timers are first expressed,...expression time. 2. Timer functionality with a single gene Although it is possible to make a timer system ...FPs, the FP timers, add an entire new dimension to monitoring localization; using FP timers, researchers...population of timer indicates the age of the pool: the higher the ratio, the older the pool. FP timers have been... hallmarks of ideal FP timers: 1. Ratiometric determination of expression time The ratio of mature to ...immature fluorescence from the FP timer is dependent on the total expression time but independent of protein ...total expression time can therefore be used to determine how long a pool of FP timers has been expressed...

...hopefully help guide policies and ultimately treatments in a timely fashion.”  With this in mind, Bourque...supporting their access to useful resources during this time. Facilitate collaborations within the scientific...highly skilled scientists, willing to volunteer their time and expertise for this cause: from virologists who...

...Taek Soon Lee Joshua Leonard Han Lim Wendell Lim Timothy Lu Michel Maharbiz Thorsten Mascher Kathleen Matthews...

...generated using standard methods individually optimized for each specific antibody in order to generate...

...target-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and Cas...

...target-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and Cas...

...histology conditions, minimum/maximum incubation time after in vivo delivery, etc.) Spark the discussion...

... slowly after introducing a cut, with residence times on DNA of approximately 6 hours. To our surprise...homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery." Elife 3 (2015): e04766...

...target-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and Cas...

...acids, their repeat-variable di-residue (RVD). Ultimately, it is this RVD that determines which single ...

...Sp Cas9 Cut Blasticidin Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance...

...determine when the optimal time to conduct an experiment begins. This can save you time in the long run ...information.  Optimize titer for the DIO/FLEx vector Once you have determined the optimal injection parameters...begin to optimize the DIO/FLEx vector. This step is essentially the same as what was used to optimize the ...used.   Following this stepwise optimization will take a fair amount of time, and it may be tempting to skip...experiments. However, by optimizing all conditions first, you will save time, frustration, and funds in...the causes of off-target expression and ways to optimize experiments to minimize it. Overview of DIO/FLEx...created with BioRender.com   Strategies for optimizing expression So now that we know spontaneous recombination...

...level of intimacy that creates the magic of a safe space. No matter why, now is a great time to find a...work-from-home and just now realizing that we can’t bide our time and wait for it to be over to work on career skills...speaking For almost any science career, there will be a time when you need to speak publicly to communicate your... by now that people get jobs at least 85% of the time because of relationships. It’s much harder to build...

...which takes time because the solution is viscous, or by autoclaving, which also takes time. Rather than... virus for my first experiment. It was a bit intimidating, but I had my lab’s protocols and some great...total, this process takes 4-7 days, not counting the time needed to expand the HEK293 cells. Check out the...screening for ITR deletion is still necessary. To save time and money, I like to miniprep 5 mL of my AAV transfer... my AAV packaging PEI transfections. It saves me time and helps me figure out if I have enough plasmid...the HEK293 cell culture supernatant because it’s time consuming to prepare the 40% PEG solution. PEG takes...AAV lysis buffer and freeze/thaw the lystate four times in a 95% ethanol and dry ice bath to release the...

...selected sgRNAs in this optimal window and ranked them based on their optimized sgRNA rules and potential...genetic screen. However, there are benefits to optimized libraries that utilize only a few sgRNAs per gene...Sanson et al., 2018). These libraries utilize optimized sgRNAs that were designed based on the rules identified...library performance. The Root and Doench lab’s optimized genome-wide CRISPRko, CRISPRi, and CRISPRa libraries... a look at the features and advantages of the optimized human CRISPR libraries. Brunello: human CRISPRko... Thus, the Brunello library proves that a few optimized sgRNAs is enough for efficient library performance...Overall the Root and Doench lab’s have proven their optimized, human genome wide CRISPRko, CRISPRi, and CRISPRa...

...this adds up to a significant time savings. You can also save yourself time if you're transforming a plasmid... each week at Addgene, so we need to be cost and time efficient. We do this by making our own competent...antibiotics to give the antibiotic resistance gene time to be expressed.  However, you can skip this incubation...

...target-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and Cas...