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We narrowed to 169 results for: TOX

Showing: 61 - 80 of 169 results
  1. Hot Plasmids February 2024

    Type
    Blog Post
    ...cells remain difficult to genetically edit due to toxicity from electroporation or poor cellular entry with...frequency (nearly 100% in some cases) and minimal toxicity, including in clinically-relevant lines such as...
  2. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...extremely useful if a desired protein product is toxic to cells at high concentrations. Despite their advantages...lower expression may be required if your protein is toxic to E. coli. Of all the vectors, pET, under the control...
  3. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...increased particle stability; however, the protein is cytotoxic precluding the long-term expression of lentiviral...vectors in producer cell lines. Identifying less toxic envelope proteins or envelope proteins that target...
  4. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...correct? Is the purified plasmid DNA viable? Or cytotoxic? How and where did things go wrong? The use of...reagents or the transfection process itself has any cytotoxic effects on the target cells. Another type of transfection...
  5. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...produce viral proteins and die, either through cytotoxicity or the immune response. The second strategy ...expression. In cell lines that can produce HIV-1 toxic proteins, CRISPR/Cas9 SAM caused apoptosis, indicating...
  6. Exploring Applications of the Bioluminescent HiBiT Tag

    Type
    Blog Post
    ...generating reactive oxygen species that can lead to phototoxicity, perturb biological processes, and compromise...Reynaud, E. G., & Shroff, H. (2017). Assessing phototoxicity in live fluorescence imaging. Nature Methods...
  7. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ... overexpression of your gene and could lead to toxicity or trouble interpreting your results, while a ...low expression.  Finally, the risks of potential toxicity and/or off-target effects (e.g. recombinase-dependent...
  8. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...problematic because excitation light can cause phototoxicity, autofluorescence, and perturb biological phenomena...the case of exciting cyan-excitable donors, phototoxicity. Another challenge to using FRET sensors comes...
  9. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    ...high 3D spatial resolution while also avoiding the toxic effects of high light doses to the cells. Thin static... its fast temporal resolution and decreased phototoxicity. Lightsheet designs that allow for multi-view...
  10. Bacterial Expression Systems

    Type
    Collection
    ...Cell-Imaging Plasmid Collection contains plasmids for non-toxic protein labeling and visualization inside living...
  11. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...penetrate 5 mm past the surface of skin and is less phototoxic. Using the FAST system, Ye’s lab could edit a...editing, reduce off-target activity, and prevent cytotoxic effects. For a more detailed review, check out...
  12. Plan Your Experiment

    Type
    Collection
    ... lowest immunogenicity and are one of the least toxic methods for in vivo viral delivery. Additionally...
  13. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...  acrIIA3   Listeria monocytogenes  pCSW65 N/A (Toxic)   None N/A   acrIIA4 Listeria monocytogenes  ...  acrIIA3 Streptococcus pyogenes  pCSW24  N/A (Toxic) pJH375  No    ...
  14. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...efficiency of viral infection. However, polybrene is toxic to some cell lines. In these cell lines, substitute... media 24 hours after infection. TIP: If viral toxicity is observed in your cell line, you may decrease...
Showing: 61 - 80 of 169 results