We narrowed to 7 results for: TOX

1. General Transfection

   Type

   Protocol

   ...µg of DNA into 500 µL of Opti-Pro SFM. Pro-Tip Endotoxins can inhibit transfection; therefore, plasmid ...plasmid DNA purification should include an endotoxin removal step. For high quality plasmid DNA, the plasmid...

2. Lentivirus Production

   Type

   Protocol

   ... optimized for each transfer plasmid. Pro-Tip Endotoxins can inhibit transfection; therefore, plasmid ...plasmid DNA purification should include an endotoxin removal step. For high quality plasmid DNA, the plasmids...

3. Protocol - pLKO.1 – TRC Cloning Vector

   Type

   Protocol

   ...efficiency of viral infection. However, polybrene is toxic to some cell lines. In these cell lines, substitute... media 24 hours after infection. TIP: If viral toxicity is observed in your cell line, you may decrease...

4. AAV Purification by Iodixanol Gradient Ultracentrifugation

   Type

   Protocol

   ...macromolecules. Importantly, iodixanol is inert and non-toxic to mammalian cells. Therefore, and unlike other ...

5. Virus Protocol - Generating Stable Cell Lines

   Type

   Protocol

   ...reduced growth rates, especially if that transgene is toxic. Eventually, the rapidly growing low-level transgene...

6. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

   Type

   Protocol

   ...reproducibility in science. Safety Warnings Sodium azide is toxic. When handling, use proper personal protective equipment...

7. AAV Production in HEK293 Cells

   Type

   Protocol

   ...post transfection. Changing the media can reduce toxicity associated with PEI and allow for the addition...