... where specific tissues, cell types, or even subcellular regions of a neuron need to be targeted. Targeting...

...been developed into retroviral vectors and 2.) a subclass of retroviruses called the lentiviruses (like ...

...and Share AAV Data with Addgene's New AAV Data HubCelebrate Open Data Day with Addgene’s AAV Data Hub!Data...

...antibodies can be isotype-specific or even isotype subclass-specific. This specificity can be used to ensure...Isotype Description Configuration and Valency Subclasses IgA Prevalent antibody in secretions (e.g. tears...should never be stored below 4 °C. Certain isotype subclasses, such as IgG3, aggregate after thawing and therefore...different species of origin, isotypes, and isotype subclasses of a primary antibody, which becomes the limiting...? You may need to buy isotype-specific or even subclass-specific secondary antibodies to allow for differentiation...

...Addgene Blog: Biosensors Luciferase Plasmids Subcellular Localization Optogenetics Plasmids Viral Service...blue/green) Imaging intraorganellar Ca(2+) at subcellular resolution using CEPIA. Nat Commun. 2014 Jun ...genetically encoded sensors for population and subcellular imaging of cAMP in vivo. Nat Methods. 2022 Nov...FRET-based cGMP biosensor SponGee: A Genetic Tool for Subcellular and Cell-Specific cGMP Manipulation. Cell Rep...for mitochondria or cytosol Hypoxia triggers subcellular compartmental redox signaling in vascular smooth... Ultrafast fluorescent voltage sensor ASAP2f Subcellular Imaging of Voltage and Calcium Signals Reveals...

...structures GFP is limited in its ability to label subcellular structures in the CNS. GFP and other FPs have...

...transductions. Cell lines should be routinely subcultured at densities that are low enough to prevent confluency...

...fused to a protein of interest to elucidate the subcellular localization of your protein of interest. Find...

... in vitro without antibiotic selection. In a subcutaneous cancer model, the researchers showed that after...

...Andrews, N.P., et al. (2019). A toolbox of IgG subclass-switched recombinant monoclonal antibodies for...

... Constructs to target mTurquoise2 to various subcellular compartments pCEP4CyPet-MAMM Cyan Mammalian...

...bait protein can even target BirA* to specific subcellular locations, such as the nuclear envelope, allowing...

...temperature variations and heat transfer at the subcellular level requires a probe with fast kinetics and...

...only includes general lab strains designed for subcloning or protein expression. If you were to include...

...multiple cellular events such as the dynamics of subcellular structures and gene expression. You must see ...

... as seen with other epigenome-editing tools. pLV hUbC-dCas9-MQ1(Q147L)-EGFP is also available for lentiviral...

...immunoprecipitation and are a common readout for subcellular fractionations, a procedure that isolates proteins...

... J., & Trimmer, J. S. (2019). A toolbox of IgG subclass-switched recombinant monoclonal antibodies for...

...antibody that you want to use to look at the subcellular localization of a protein of interest. Conveniently...

...Fluorescent Protein Guide Pages Find Plasmids for Subcellular Localization Find Fluorescent Protein Empty Vectors...