We narrowed to 218 results for: Ung;
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TypeBlog Post...retired! Conversely, cells younger than the 3rd – 4th passage are too young and should not be used for...
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PITChing MMEJ as an Alternative Route for Gene Editing
TypeBlog Post...insert FLAG tags into the genome of the pathogenic fungus Aspergillus fumigatus, which has been difficult...microhomology-mediated end joining in Aspergillus fumigatus.” Fungal Genet Biol. 86: 47-57. PMID: 26701308. Additional... -
Tips for arabidopsis transformation
TypeBlog Post... as anthocyanin production, or disease, such as fungal growth. Healthy plants produce lots of seeds. More...mean more chances to recover a transformant. Use young plants: If you transform early, your plants will... -
Tips for Screening with Yeast Two Hybrid Systems
TypeBlog Post...model organisms, including bacteria, alternative fungi (C. albicans, pC2HB) (7) and mammalian cells. If...codon adapted two-hybrid system for the pathogenic fungus Candida albicans. Nucleic Acids Res. 2010;38(19... -
Save Time with Transient Plant Leaf Transformations
TypeBlog Post...overcome this problem in C. roseus by working with young seedlings and optimizing several other aspects of...re-invent the wheel. Test different leaf ages Usually younger leaves work better. Not every leaf is equally susceptible... -
Overcoming the AAV Size Limitation for CRISPR Delivery
TypeBlog Post...Gootenberg JS, Abudayyeh OO, Franklin B, Kellner MJ, Joung J, Zhang F (2017) RNA editing with CRISPR-Cas13....Park SW, Kim D, Kim K, Cho H-Y, Song DW, Lee KJ, Jung MH, Kim S, Kim JH, Kim JH, Kim J-S (2017) In vivo... -
CRISPR 101: Targeting RNA with Cas13a (C2c2)
TypeBlog Post...within cell-free genomic DNA, and detect COVID-19 (Joung, Ladha et al., 2020). Cas13a in mammalian cells ... 26593719. PubMed Central PMCID: PMC4660269. 7. Joung J, Ladha A, et al., ‘Point-of-care testing for COVID... -
Adeno-associated Viruses (AAVs) for Genome Editing
TypeBlog Post...14704360. PubMed Central PMCID: PMC373311. 3. Kim, Jung-Sik, et al. "Epitope tagging of endogenous genes...18784188. PubMed Central PMCID: PMC2577350. 4. Kim, Jung-Sik, et al. "Intact cohesion, anaphase, and chromosome... -
PiggyBac-ing Through the Genome Editing Field
TypeBlog Post..., J. D. Sander, J. Staber, S. J. Wheelan, J. K. Joung, P. B. McCray, F. D. Bushman, P. L. Sinn, and N....Functional Gene Correction for Cystic Fibrosis in Lung Epithelial Cells Generated from Patient iPSCs.,”... -
Finding Your Perfect Job After University
TypeBlog Post...the procedures in the lab are done with robots. Young scientists going into this field don’t really get...position in academia. It is therefore important for young scientists and PhD students to also explore a range... -
Building Global Connections with the International Mentorship Program USA-EUROPE
TypeBlog Post...Corsino. When someone says “internationalization” young hearts often shake with the fear of uncertainty....entrepreneurial visions thereby opening new frontiers for young talent around the world. The wealth of knowledge... -
FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution
TypeBlog Post...this represents a major downside in optogenetics (Belzung et al., 2014). This could be overcome by fusing...28.28 (2008): 7025-7030. PubMed PMID: 18614669. Belzung, Catherine, Marc Turiault, and Guy Griebel. "Optogenetics... -
A History of Genome Engineering in Popular Culture
TypeBlog Post... a nucleotide triplet and when several ZFNs are strung together, it can read many base pairs simultaneously...Pickle CS, Ralston EJ, Lee AH, Amora R, Miller JC, Leung E, Meng X, Zhang L, Rebar EJ, Gregory PD, Urnov ... -
Typing CRISPR Systems
TypeBlog Post...Gootenberg, J. S., Essletzbichler, P., Han, S., Joung, J., Belanto, J. J., Verdine, V., Cox, D. B. T.,...Abudayyeh, O. O., Franklin, B., Kellner, M. J., Joung, J., & Zhang, F. (2017). RNA editing with CRISPR-Cas13... -
Cancer and the Immune System: Deciphering the Relationship
TypeBlog Post...the first ever CRISPR-based clinical trial in 18 lung cancer patients. This study used CRISPR gene editing...for acute lymphoblastic leukaemia in children and young adults: a phase 1 dose-escalation trial." The Lancet... -
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation
TypeBlog Post...Pancreatic β-cells [16] Intestinal tissue [17] Lung epithelial cells [18] Red blood cells [19, 20] ...18. Huang, S.X., et al., Efficient generation of lung and airway epithelial cells from human pluripotent... -
Optogenetics + CRISPR, Using Light to Control Genome Editing
TypeBlog Post...Magnet photoswitchable proteins derived from the fungal photoreceptor, Vivid (VVD, N. crassa) (Kawano et.../doi.org/10.1002/anie.201914575 Zhou XX, Zou X, Chung HK, Gao Y, Liu Y, Qi LS, Lin MZ (2017) A Single-... -
Feng Zhang Multiplexed Overexpression of Regulatory Factors (MORF) Collection
TypeCollection...transcription factor atlas of directed differentiation. Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer... -
Cancer Research Plasmids and Resources
TypeCollection...publications, focused on somatic variants found in lung adenocarcinoma as well as variants found across ... -
CRISPR Plasmids - Zebrafish
TypeCollection... In vitro transcription none, need Cas9 plasmid Joung Do you have suggestions for other plasmids that ...