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Showing: 61 - 80 of 215 results
  1. Tips for arabidopsis transformation

    Type
    Blog Post
    ... as anthocyanin production, or disease, such as fungal growth. Healthy plants produce lots of seeds. More...mean more chances to recover a transformant. Use young plants: If you transform early, your plants will...
  2. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...model organisms, including bacteria, alternative fungi (C. albicans, pC2HB) (7) and mammalian cells. If...codon adapted two-hybrid system for the pathogenic fungus Candida albicans. Nucleic Acids Res. 2010;38(19...
  3. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...within cell-free genomic DNA, and detect COVID-19 (Joung, Ladha et al., 2020). Cas13a in mammalian cells ... 26593719. PubMed Central PMCID: PMC4660269. 7. Joung J, Ladha A, et al., ‘Point-of-care testing for COVID...
  4. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ...overcome this problem in C. roseus by working with young seedlings and optimizing several other aspects of...re-invent the wheel. Test different leaf ages Usually younger leaves work better. Not every leaf is equally susceptible...
  5. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...Gootenberg JS, Abudayyeh OO, Franklin B, Kellner MJ, Joung J, Zhang F (2017) RNA editing with CRISPR-Cas13....Park SW, Kim D, Kim K, Cho H-Y, Song DW, Lee KJ, Jung MH, Kim S, Kim JH, Kim JH, Kim J-S (2017) In vivo...
  6. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...14704360. PubMed Central PMCID: PMC373311. 3. Kim, Jung-Sik, et al. "Epitope tagging of endogenous genes...18784188. PubMed Central PMCID: PMC2577350. 4. Kim, Jung-Sik, et al. "Intact cohesion, anaphase, and chromosome...
  7. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ..., J. D. Sander, J. Staber, S. J. Wheelan, J. K. Joung, P. B. McCray, F. D. Bushman, P. L. Sinn, and N....Functional Gene Correction for Cystic Fibrosis in Lung Epithelial Cells Generated from Patient iPSCs.,”...
  8. Finding Your Perfect Job After University

    Type
    Blog Post
    ...the procedures in the lab are done with robots. Young scientists going into this field don’t really get...position in academia. It is therefore important for young scientists and PhD students to also explore a range...
  9. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ... a nucleotide triplet and when several ZFNs are strung together, it can read many base pairs simultaneously...Pickle CS, Ralston EJ, Lee AH, Amora R, Miller JC, Leung E, Meng X, Zhang L, Rebar EJ, Gregory PD, Urnov ...
  10. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...the first ever CRISPR-based clinical trial in 18 lung cancer patients. This study used CRISPR gene editing...for acute lymphoblastic leukaemia in children and young adults: a phase 1 dose-escalation trial." The Lancet...
  11. Typing CRISPR Systems

    Type
    Blog Post
    ...Gootenberg, J. S., Essletzbichler, P., Han, S., Joung, J., Belanto, J. J., Verdine, V., Cox, D. B. T.,...Abudayyeh, O. O., Franklin, B., Kellner, M. J., Joung, J., & Zhang, F. (2017). RNA editing with CRISPR-Cas13...
  12. E11 Bio PRISM Collection

    Type
    Collection
    ... J.Y., Chan, S.W., Damstra, H.G.J., Leible, D., Leung, K.S., Magno, C.A., Meeran, A., Michalska, J.M.,...
  13. Synthetic Biology - Overview

    Type
    Collection
    ...Model Organism Bacterial Plant Yeast Mammalian Worm Fungal Algal Featured SynBio Deposits Alper Lab Y. Lipolytica...
  14. AAV for Neuronal Tracing

    Type
    Collection
    ...DC, Barnard RJ, Mori T, Finke S, Conzelmann KK, Young JA, Callaway EM. 2007b. Monosynaptic restriction...
Showing: 61 - 80 of 215 results