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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 376 results for: bre

Showing: 61 - 80 of 376 results

  2. COVID-19 Resources

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    Collection
    ...related to SARS-CoV-2 research during the current outbreak: Cell Press Coronavirus Resource Hub COVID-19 ...

  3. CRISPR 101: Cytosine Transversion Editors

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    ... (ABEs) chemically modify target bases without breaking the DNA backbone, making them efficient and precise...nickase (nCas9) to avoid introducing double-strand breaks. The only things base editors really need from ...also called apurinic or apyrimidinic sites and abbreviated as AP sites. To explore this further, the team...that is known for sometimes causing double-strand breaks (DSBs). The Fei-Long Meng Lab did in-depth profiling...strand is excluded as ssDNA. DSB Double-Stranded Break A cut to both strands of DNA. Indel Insertion/... (2024). C-to-G editing generates double-strand breaks causing deletion, transversion and translocation...

  4. 5 Tips for Troubleshooting Viral Transductions

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    Blog Post
    ...negatively-charged membranes (Davis et al., 2002). Polybrene is one such cationic reagent that is commonly ...efficiency by 10-fold (Davis et al., 2002). However, polybrene is highly sensitive to freeze-thaw cycles and ...be stored in single-use aliquots. Never use a polybrene solution that has been thawed more than once. ...in cells that are sensitive to the toxicity of polybrene (e.g., hematopoietic or primary cells), and has...., Jeffrey R. Morgan, and Martin L. Yarmush. "Polybrene increases retrovirus gene transfer efficiency ...

  5. Five Popular Model Organisms

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    Blog Post
    ...1970s when Sydney Brenner brought it into the lab to study neuronal development (Brenner, 1973). C. elegans...scientific research. Yes, the same kind we use in breads and other baked goods! Yeast is cheap, simple and...maintained as they are housed in large groups, easily bred, and produce 50-300 eggs at a time. Zebrafish embryos...developed for emerging model organisms! References Brenner, Sydney. "The genetics of Caenorhabditis elegans...

  6. Caltech Systemic Capsids

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    ...strains. **AAV1-X1 has not been tested in rats. Abbreviations: AAV, adeno-associated virus; GRE, gene regulatory...

  7. Embracing Serendipity: A Crucial Element in the PhD Journey

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    ...In the pursuit of knowledge and groundbreaking discoveries, the world of academia often emphasizes structured... journey — serendipity. The best scientific breakthroughs often occur when the unexpected infiltrates ...details of a specific research question. However, breakthroughs often happen when we allow ourselves moments...that is adaptable, creative, and poised for groundbreaking discoveries. So, on your academic journey, ...

  8. Intro to Our Summer SciComm Series!

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    Blog Post
    ...using dog breeds as an example adults 45-55 yrs old who breed or are interested in breeding dogs who ...enjoy listening to podcasts A series of Tweets breaking down a new publication that could advance treatments...

  9. Plasmids for Endogenous Gene Tagging in Human Cells

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    Blog Post
    ...repair (HDR) process following the double strand break CRISPR/Cas9 makes at the target site. The whole ...Cas9 during transfection. Making the double strand break and introducing the tag through homology directed...work. Figure 3: Inducing the double strand break and introducing the tag. Created with BioRender....provides an alternative to HDR as the double-strand break repair pathway, relying instead on microhomology-mediated...

  10. Adapting Toehold Switches to Detect Zika Virus

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    Blog Post
    ... This changed in January with the report of an outbreak of the virus in Brazil that was correlated with... a more field-ready format when the Zika virus outbreak was reported, and we felt that, given the critical...underscored by the potential scale of the emerging outbreak, as many as 2.2 billion people globally live in...discrimination is especially important in the current outbreak because of the neurological symptoms associated...

  12. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

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    Blog Post
    ... reaching their targets. The AAV9 serotype can breach the BBB following injection into the bloodstream...mice. They took a classic genetics approach and interbred C57BL/6J and BALB/cJ mice to map the inheritance... PHP.B permissiveness. The first generation of outbred mice (F1) had half the level of CNS PHP.B transduction...C57BL/6J parent. The second generation (F2) of outbred mice also showed a Mendelian inheritance pattern...

  13. Bacterial Expression Systems

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    ...Cas9 from S. pyogenes for inducing double stranded breaks. For a more comprehensive list of our bacterial...

  14. CRISPR Pooled gRNA Libraries

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    ...Doench and Root 3rd 4 2,852 Broad GPP activation Calabrese p65-HSF 92379 (Set A) 92380 (Set B) Activation...

  15. Viral Vectors 101: Optogenetic Tools

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    Blog Post
    ... and downstream signaling cascades (reviewed in Abreu & Levitz, 2020), including light-sensitive chimeric...delivering your light stimulus. This can make or break your experiments!  First, closely read the publication...! Resources and references Recommended reading Abreu, N., & Levitz, J. (2020). Optogenetic Techniques.... https://doi.org/10.1038/nmeth.1808 References Abreu, N., & Levitz, J. (2020). Optogenetic Techniques...

  16. CRISPR 101: Off-Target Effects

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    Blog Post
    ...nuclease that either introduces a ssDNA nick, a dsDNA break, or is engineered not to cleave DNA and instead ... other, this will result in a DNA double strand break (DSB), the event required for frameshift and knock-in...on an off-target site, it will generate a ssDNA break which can easily be repaired by cells with a near...DNA by the NHEJ pathway during DNA repair of the break to allow for site-specific detection downstream....

  17. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

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    Blog Post
    ...-directed repair (HDR) to correct double-strand breaks (DSBs). DSB repair by HDR facilitates in-frame ...epitope tags at target genes in mammalian cells (Bressan et al. 2017; Liang et al. 2017). Current methods...daydreaming about sunny days in the UK.   References 1. Bressan RB, Dewari PS, Kalantzaki M, Gangoso E, Matjusaitis...polymerase-mediated dislodging of Cas9 from double strand DNA breaks." bioRxiv(2018): 300962. PubMed PMID: 29979968....

  18. With an Eye Towards the Future, We Look Back at the March for Science

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    ...about possible broad changes to policies seems to break everyday. Many researchers and supporters of science...Vanessa Sung is currently a PhD student studying breast cancer at the Goodman Cancer Research Centre at...’s used to fight the myths that abortions cause breast cancer and infertility or to argue against unnecessary...scientists, we work tirelessly in the lab where the groundbreaking research happens, sometimes forgetting how ...

  19. A Primer on Optogenetics: Introduction and Opsin Delivery

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    ...stereotaxic surgery relies on identification of the bregma and lambda sutures and subsequent determination...coordinates based on your own method of identifying bregma but it is best to spend the time practicing your...advantage is you can “zero” your coordinates at bregma which allows you to quickly and reliably locate... limited by the mouse line itself. Generation, breeding, and maintenance of a mouse line is also an expensive...

  20. Plasmids 101: How to Name Your Plasmid in 3 Easy Steps

    Type
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    ...case letter to the beginning of their insert abbreviation to specify what species it is. Example: ‘h’ ...amino acid mutation is to list the one letter abbreviation of the wild type amino acid immediately followed... Methionine (Met) followed by the one letter abbreviation of the mutated amino acid currently at that ...
Showing: 61 - 80 of 376 results