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We narrowed to 166 results for: control GFP

Showing: 61 - 80 of 166 results

  1. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...pSNAPf-Cox8A Control Plasmid SNAP-tag Control for mitochondrial localization 101124 pSNAPf-H2B Control Plasmid...SNAP-tag Control for nuclear localization 101123 pSNAPf-ADRβ2 Control Plasmid SNAP-tag Control for cell...pCLIPf-H2B Control Plasmid CLIP-tag Control for nuclear localization 101130 pCLIPf-Cox8A Control Plasmid ...CLIP-tag Control for mitochondrial localization 101125 pCLIPf-NK1R Control Plasmid CLIP-tag Control for cell...pMCP-GPI Control Plasmid ACP/MCP-tag Control for cell surface localization 101131 pACP-GPI Control Plasmid...-tag Control for cell surface localization 101128 pACP-ADRβ2 Control Plasmid ACP/MCP-tag Control for cell...comprehensive comparison to GFP, please refer to NEB's comparison of SNAP-tag, CLIP-tag, and GFP . Technology SNAP-tag...

  2. Hot Plasmids: Fall 2025

    Type
    Blog Post
    ...fused to GFP forms fluorescent biomolecular condensates. A GFP-nanobody fused to the killswitch (GFP-nb–KS...protein of interest or targeted to a GFP fusion protein using an anti-GFP nanobody (Figure 4). Once incorporated...deposited from this paper, a large menu of tool and control constructs is available to choose from! Find plasmids... within a lox-STOP-lox sequence, followed by the EGFP, and the TEV protease. Once the recipient cells ... excises the DsRed-STOP to trigger expression of EGFP. Figure 2: Schematic of the MitoTRACER system...

  3. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ...or smaller. Some examples include a reporter like GFP, some smaller CRISPR-Cas proteins such as SaCas9,... promoters are upstream or 5’ of the gene they control. Promoters can be organism or tissue type-specific...recombinase, are used to spatially and temporally control transgene expression. Lox sites are directional...availability of Cre, lox sites lets researchers control the spatial and temporal activation of a transgene...

  4. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    ...plasmids contain an origin of replication (which controls the host range and copy number of the plasmid)... that scientists can purify and study it in a controlled setting. Read more: Plasmids 101: Protein Tags...cell Plasmids 101: Green Fluorescent Protein (GFP) Plasmids 101: Luciferase Monitor the level of...environment Produce enzymes that will make specific, controlled changes to an organism’s genome (genome engineering...

  5. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ...Fluorescent Proteins Control Protein Expression with Cre-lox Control Protein Activity with GFP Resources at ...transmitted by intermediate messengers. Optical control of kinase activity can provide greater spatiotemporal...There is no common method for creating optically controlled kinases. The Solution: In Zhou et al, the Lin... the delayed effects because you can precisely control the timing of kinase activity. 2. Studying negative...photoswitchable kinases in vivo not only allows for precise control of a signaling pathway in the context of a whole...addition, it could be possible to use pdDronpa to control other kinases and proteins. Zhou et al suggest ...Pengpeng Li, Kang Shen, and Michael Z. Lin. "Optical control of cell signaling by single-chain photoswitchable...

  6. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ...check for GUS-like activity of your tissue. Run a control with Agrobacterium lacking the GUS gene since plants...promoter activity, for example. Useful positive controls could include: pSB96 - pL2_pSB90_2x35S::fLUC-I...tMAS::rLUC-I::pMAS. Fluorescent proteins (FP, e.g. GFP) can be used in many ways. Often a FP is fused to.... "Evaluation and comparison of the GUS, LUC and GFP reporter system for gene expression studies in plants...

  7. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    ...Using an epifluorescence microscope and GFP fusion proteins controlled by promoters specific to the immune... to track where GFP labelled B cells were in mounted slices of these organs. These GFP labelled cells ...enabled by fluorescent proteins The optimization of GFP and its derivatives by the late Roger Tsien changed...immune lineage, scientists could easily track GFP expressing cells. For example, by harvesting lymph nodes... the infiltration of neutrophils expressing LysM-GFP (green) into a draining lymph node after infection...Laser include: Blue-green fluorescent proteins like EGFP Yellow-orange fluorescent proteins like TagRFP, ...

  9. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    ... from the default GFP (green) to RFP, CFP, or YFP fluorescence. In Brainbow 2.1, GFP is tagged with a ...and activate fluorescence of the downstream OFP, GFP, or RFP. Cells that express the construct but do ...use in Drosophila melanogaster and is under the control of Flp recombinase. The XFPs in Flybow are membrane-tethered...generates cells that randomly express either nuclear GFP, membrane CFP, cytoplasmic YFP, or cytoplasmic RFP...incorporates the highly photostable fluorophores mOrange2, EGFP, and mKate2 (red) to solve the problematically low...

  10. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

    Type
    Blog Post
    ...fluorescent protein reporter under the control of promoters that control cell cycle gene expression.  Additional...-up approach will allow me to create a fully controllable and reproducible set up,” Bernach explains. ...containing unstable fluorescent proteins under the control of promoters responsive to water or nutrient levels...fluorescent protein topics page Get the basics on GFP Find microbiology blog posts Resources on Addgene.org...variant library is monitored by fusing the variants to eGFP followed by an internal ribosomal entry site and...a variant has wild-type levels of abundance, the eGFP will be stable and fluoresce. But if the variant...unstable, it will be degraded, leading to reduced eGFP fluorescence. Cells expressing the library are then...

  11. Serotype Testing AAV

    Type
    Collection
    ... AAV available from Addgene's viral service. Control EGFP vectors in various serotypes for serotype testing...list of all 100 µL sized sample, please see our Control AAV Inventory . The catalog ID for the 20 µL sized...AAV1). AAV Vectors for Serotype Testing pAAV-CAG-GFP (Plasmid #37825) Description : Ready-to-use AAV in...plasmid 37825 (deposited by Edward Boyden ) and direct GFP expression from the CAG promoter. For information... catalog item, including the titer and quality control, visit the material page (linked for each catalog... 37825-AAVrg.T 20 µL $ 150 Add to Cart pAAV-hSyn-EGFP (Plasmid #50465) Description : Ready-to-use AAV ...

  12. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...selectable marker and a desired genetic payload (ex: GFP). Thus, cells can be both sorted using FACS and isolated...allows translation of the reporter gene resulting in GFP signal. With the addition of standards, the assay...protein. The expression of this fusion is under control of the arabinose-inducible promoter. Cells are ...

  13. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type
    Blog Post
    ...right negative control is critical to the success of BiFC experiments. The ideal control is a mutated variant... work. You will also need to generate negative control constructs — more on this later. For more on constructing...check out our article: Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection. Cellular... measure the fluorescence of a single FP. Your control construct may generate a modest level of fluorescence...intensity of your BiFC constructs to your negative control to determine whether your PIPs actually do interact...has been compromised (Kodama and Hu, 2012). Such controls are necessary because split FPs can associate,...fragments themselves cannot be used as negative controls because their expression level, stability, and...

  14. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    ...toxicity or burden relative to a fluorescent protein control. We also added the toxin-antitoxin stability cassette...P2A-linked accessory genes gvpNJKFGWV marked with Emerald GFP. These plasmids can be used directly in transient...gating for the top quartile (or higher) of BFP and GFP producing cells. After FACS, return the cells to ...Biomolecular Tools for Acoustic and Magnetic Imaging and Control of Cellular Function. Biochemistry 56, 5202–5209...

  15. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type
    Blog Post
    ... No spatiotemporal control. Many PPI detection methods lack spatiotemporal control or an “on/off switch...partners if they are enriched relative to controls using Split-BioID-GFP and BioID run on six unrelated proteins... by creating Split-BioID, a spatiotemporally controllable version of the proximity-dependent biotinylation...method Enter Split-BioID, a spatiotemporally controllable version of the BioID protocol. The critical ...methods, including BioID, is its spatiotemporal controlled labeling of interacting proteins; nearby proteins...

  16. Tetracycline Inducible Expression

    Type
    Collection
    ...Iwasato 63704 pRetroX GFP T2A Cre Retroviral vector for dox-inducible expression of GFP T2A Cre recombinase...Eric Kowarz 96930 XLone-GFP Tet-On PiggyBac vector for inducble expression of EGFP Tet-On 3G rtTA TRE3GS...171123 pLVX-TetOne-Puro-GFP Lentiviral Tet-On vector for inducible expression of EGFP Tet-On 3G rtTA TRE3GS... for Tet-controlled expression (On or Off) None Tight TRE Markus Ralser 64238 pTet-IRES-EGFP Lentiviral...Lentiviral plasmid for Tet-controlled expression of transgene of interest with EGFP (On or Off) None TRE, miniCMV...and repressible promoters are powerful tools to control gene expression and study biological targets. The...popular choices for rapid, efficient, and reversible control of gene expression in eukaryotes. To avoid secondary...

  17. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    ...comes either with or without mCherry and GFP fluorescent controls. Alternatively, you can order individual...streamline identification. While the Zhang lab controlled their multiplicity of infection so that a single...disease depends on both genetic and expression control systems to mimic illness on a molecular level. ...

  18. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    ...function of Your Gene Of Interest (YGOI) in a controlled environment under various conditions. The first...great for overexpressing YGOI in an isolated, controlled environment. In most cases bacterial expression...using a reporter vector to tag YGOI with lacZ and/or GFP (e.g. pPD80_08) and following the expression pattern... allow you to visualize YGOI in vivo (try pcDNA3-EGFP). If you can get away with generating transiently...

  19. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ... should contain a reporter DNA sequence (such as GFP) in the sense orientation, followed by coding sequence...transduced reporter gene. Generating a FLEx switch to control expression of an optogenetic reporter would ensure...et al. Nat Biotech 2003): In the presence of Cre, GFP expression is lost, allowing for rescued expression...

  20. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ...protein. With this control set, you should see your target protein IP’d in the positive control sample but not...permanently altering the structure of the protein.  Controls As with any experiment, it is critical to include...include proper positive and negative controls for an IP. When possible, perform the IP in parallel with ...interest and a sample that does not. Common negative controls include knockout cell lines or tissues that do... not in the negative control, confirming that the capture antibody is functioning as expected. In order...perform parallel IPs with beads only and an isotype control antibody. Alternative applications Oftentimes, ...In cases such as this, an epitope such as c-Myc, GFP or V5 can be used to tag the protein of interest ...
Showing: 61 - 80 of 166 results