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We narrowed to 987 results for: des

Showing: 61 - 80 of 987 results

  2. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...Green, Alexander A., et al. "Toehold switches: de-novo-designed regulators of gene expression." Cell 159.4...Specifically this includes primer directed isothermal RNA amplification, new toehold switch designs, CRISPR/Cas9...is an Assistant professor at the Biodesign Center for Molecular Design and Biomimetics and the School of...development of diagnostics. Mostly transmitted by Aedes mosquitoes (aegypti and albopictus) and, aided by... toehold switches, which allow for the rational design of RNA sensors that can detect virtually any RNA...areas conducive to the spread of the virus by the Aedes mosquito (Messina et al, 2016). We quickly assembled...spread out the risk for the project by initially designing and ordering several dozen versions of the toehold...

  3. Rett Syndrome

    Type
    Collection
    ...in transcriptional repression, activity dependent de-repression, chromatin remodeling, gene activation...species (mouse or human) Mutation ID Plasmid Description Gene/Insert Mutations PI Return to top External...BrainSpan Atlas of the Developing Human Brain - includes developmental transcriptome information, prenatal...

  4. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    ...https://doi.org/10.1101/2020.04.16.045419.  Wrapp D, De Vlieger D, Corbett KS, Torres GM, Wang N, Van Breedam...Zimmermann et al., 2020). This selection platform was designed so that any standard lab can quickly select for...strongly to your target protein. The Seeger lab designed 3 different mRNA sybody libraries for use in the... randomized with a defined set of different trinucleotides (corresponding to different amino acids) in...for binding to the target protein. The protocol describes going through two rounds of phage display to increase...interest. The Seeger lab used the ELISA technique described in their protocol because it is less susceptible...

  5. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    ... JA, Han Y-C, Ogrodowski P, Crippa A, Rekhtman N, de Stanchina E, Lowe SW, Ventura A (2014) In vivo engineering...targeted organs or tissues with Cas9 and a gRNA designed to target a mutated gene. In a Scientific Reports...Center are now available at Addgene along with a description of their experimental protocol. The three plasmids...plasmid tools, find more plasmids, CRISPR technology guides, FAQs, and CRISPR resources on Addgene's CRISPR...Additional resources on the Addgene blog How to Design Your gRNA for CRISPR Genome Editing by John Doench...

  6. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    .... When contamination is suspected, we proceed to de novo assembly of the unmapped reads, employing our...transduced cells whenever possible. This post will describe our workflow for viral genome sequencing (VGS)...

  8. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ... Special thanks for Dr. Dan Stone and Harshana de Silva Feelixge at Fred Hutchinson Cancer Center for...plasmids to produce AAV: 1) the RepCap plasmid which provides the AAV replication (rep) and capsid (cap) genes...replication; and 3) the transfer plasmid which encodes a transgene of interest that’s packaged into the...

  9. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...392–400. https://doi.org/10.1038/s41434-018-0028-5 de Backer, M. W. A., Brans, M. A. D., Luijendijk, M....of negative data.  Negative AAV data is highly desirable  In a survey we conducted to improve our Addgene...every researcher experiences and that it often provides incredibly valuable insight into one’s work. As... Hub is here to help!  The Addgene Data Hub is designed to help scientists understand how our vectors ...standards (clear and detailed methods and image descriptions, a minimum n = 2, and well labeled high- and...the Data Hub. These minimum data standards are designed specifically to allow scientists to evaluate how...

  10. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...to sickle cell disease. Hoban MD, Orkin SH, Bauer DE. Blood. 2016 Feb 18;127(7):839-48. PubMed PMID: 26758916... but without sustained transgene expression. To design a viral vector system that could do both, Addgene...infect. Finally, the transfer plasmid encoded the desired transgene flanked by HIV-1 long terminal repeats... knockout cell lines. Many CRISPR plasmids are designed for lentiviral use, including Addgene’s top requested... check out our lentiviral plasmid and biosafety guides before beginning your experiment. Let us know how...

  11. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Pietro De Camilli 194005 VPS13D1-1576-(Y1253S) VPS13D GFP CMV Spinocerebellar Ataxia Pietro De Camilli...145 SYNJ1 Flag CMV Parkinsonism, early-onset Pietro De Camilli 22292 pcDNA3-FLAG-Synaptojanin 1-170 SYNJ1...SYNJ1 Flag CMV Parkinsonism, early-onset Pietro De Camilli 22293 pEGFPC1-FLAG-Synaptojanin 1-145 SYNJ1 GFP... GFP, Flag CMV Parkinsonism, early-onset Pietro De Camilli 22294 pEGFPC1-FLAG-Synaptojanin 1-170 SYNJ1...SYNJ1 GFP, Flag CMV Parkinsonism, early-onset Pietro De Camilli 22563 Flag-HA-UCHL1 UCHL1 HA, FLAG Parkinson's...inserted at position aa 1914 CMV Parkinsonism Pietro De Camilli 118791 pHBS1383 TDP43 RRM-GFP FYW-S TARDBP...delta1235-1748 VPS13C Halo CMV Parkinsonism Pietro De Camilli 187767 MAC_C_CSF1R CSF1R MAC Hereditary diffuse...

  12. Luciferase Plasmid Collection

    Type
    Collection
    ...LuxSit-i and neoLux1.2 : Artificial luciferases desined de novo using deep-learning approaches. Split Luciferase...Structurally destabilized firefly luciferases : A set of three increasingly destabilized mutants which...mammalian systems. ID Plasmid Luciferase Type(s) Description PI 64784 pGL3-Basic-IRES Firefly Insertion of...plasmids. ID Plasmid Luciferase Type Promoter Description PI 60226 AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc...your regulatory element of interest. ID Plasmid Description Gene/Insert PI Return to Top Other Highlighted...

  13. An Introduction to Adenovirus

    Type
    Blog Post
    ...adenoviral vector infected cells does not require de novo viral gene expression: Implications for gene...a physiological effect of SARS-CoV-2 toward destabilizing one’s natural immune response to Adenovirus ...viruses infecting humans (from seven species, designated as letters A–G) exist. The life cycle of AdVs...short-lived. It is worth noting that it is possible to design a vector that achieves persistent transgene expression...

  14. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...science.aaq0180  Fine EJ, Appleton CM, White DE, Brown MT, Deshmukh H, Kemp ML, Bao G (2015) Trans-spliced ...as potent and specific? The two AAV strategies described above showed successful target modification, indicating...from Streptococcus thermophilus and a rationally-designed truncated Cas9. Unfortunately, certain drawbacks... approximately 1350 amino acids in size, which includes SpCas9, and shortern orthologs approximately 1000...Split inteins are a pair of naturally occuring polypeptides that, when at the ends of two proteins, mediates... proof-of-concept split intein SpCas9 which had modest editing rates in HEK-293T cells when compared to...capabilities of full-length SpCas9. This set of plasmids includes AAV-Cas9C-VPR for targeted gene activation. Split...

  15. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    ... out to me were: Decrease the Number of Postdocs De-incentivize going into PhD programs as the standard...environments. Slide from Paula Stephan’s Talk (All slides can be found here). Importantly, the panel offered... joining long programs if it is obvious that a desired career outcome is not easily achieved. Of note...clearly not equitable to that of other trainees despite the fact that the underrepresented individual is...

  16. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...Brugman, M. H., Pike-Overzet, K., Chatters, S. J., de Ridder, D., Gilmour, K. C., Adams, S., Thornhill,...been a staple in molecular biology for over three decades. Widely used across various research applications...and human papillomavirus (HPV), to name a few (Desfarges & Ciuffi, 2012). One family of viruses, retroviridae... an example.  The lentiviral transfer plasmid encodes your insert of interest, flanked by long terminal...Specifically for our knowledge here, the Pol gene encodes three separate enzymes that are proteolytically...lentiviral particles.  The envelope plasmid typically encodes the vesicular stomatitis virus G protein (VSV-G...the retroviral life cycle for a more detailed description). Since the integrated portion does not contain...

  17. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ... 26 recombinant antibodies (rAbs) either produced de novo using yeast display technology or produced from...response, tissue repair, and kidney development. Despite their ubiquity and significance, these receptors...available through Addgene’s repository. The collection includes 26 unique antibodies that bind discrete subunits...and migration, initiating intracellular signal cascades, and allowing agile responses to mechanical forces...

  18. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...26224555. PubMed Central PMCID: PMC4884002. 10. Boer, E. de, Rodriguez, P., Bonte, E., Krijgsveld, J., Katsantoni...Northern blotting Cell surface labelling Attaching polypeptides to a substrate Flow cytometry/fluorescence-activated...your downstream application. Biotin labelled oligonucleotides can be used in a multitude of applications...commonly used this method to attach proteins or polypeptides to a solid substrate (Holden and Cremer, 2003...

  19. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ...University of Oviedo, the European University Miguel de Cervantes, the University of Navarra, and the University...together with the universities, a bright future. IMP provides matched one-on-one international mentorship, with...recommended, a mentor with international experience provides his/her mentees with a broader, first-hand vision...

  20. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...X, Scott DA, Mikkelson T, Heckl D, Ebert BL, Root DE, Doench JG, Zhang F. 2014. Genome-Scale CRISPR-Cas9...also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The CRISPR revolution shows... store a record of invading phages but also to destroy the phages upon re-exposure. More specifically,...complex binds to the foreign DNA and cleaves it to destroy the invader ( Figure 1 ). Figure 1: An overview...in vitro. In 2013, Cong et al. and Mali et al. described CRISPR-based genome editing in mammalian cell ... of new applications. The first CRISPR papers described two main categories of genome edits. First, the...very low off-target activity due to rationally designed mutations. Researchers have expanded the CRISPR...
Showing: 61 - 80 of 987 results